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The map label for this gene is leuA [H]

Identifier: 218708418

GI number: 218708418

Start: 379614

End: 381161

Strand: Reverse

Name: leuA [H]

Synonym: VS_0380

Alternate gene names: 218708418

Gene position: 381161-379614 (Counterclockwise)

Preceding gene: 218708428

Following gene: 218708417

Centisome position: 11.55

GC content: 45.8

Gene sequence:

>1548_bases
ATGAACGATCAAGTAATAATTTTTGATACTACCTTACGTGACGGCGAGCAAGCATTGGCTGCAAGCCTTACGGTAAAAGA
GAAGTTACAGATCGCTTATGCTCTTGAGAGACTGGGTGTAGATGTTATCGAAGCTGGCTTCCCTATTTCTTCTCCGGGTG
ATTTTGAGTCAGTACAAACCATCGCTAAACACATCAAAGACAGCCGTATTTGTGCGCTTTCTCGTGCCGTAGCGAAAGAT
ATCGATGCCGCTGCCGAAGCGCTTAAAGTTGCTGACCAGTTCCGTATTCATACCTTCATCTCAACATCAACGGTTCACGT
ACAAGACAAATTACGCCGCAGCTACGATGACGTAGTAGAGATGGCAGTGAAAGCAGTCAAGCACGCTCGTAACTATACCG
ATGATGTTGAATTCTCTTGTGAAGATGCCGGCCGTACCCCTATCGACAACCTATGTCGCATGGTTGAAGCCGCGATTAAC
GCAGGCGCGAAAACCATCAACATTCCAGACACAGTGGGCTACACAGTACCAAATGAGTTTGGTGGCATTATCAAGACGCT
ATTCGATCGCGTACCCAACATCGACCAAGCGATCATCTCTGTTCACTGTCACGATGACTTAGGTATGTCGGTTGCAAACT
CAATTGCTGCTGTGCAAGCGGGCGCTCGTCAAATTGAAGGCACAATCAATGGTATTGGCGAACGTGCTGGTAACTGTTCT
CTAGAAGAAATCGCAATGATCATCAAAACTCGTCAAGAGCTTTTGGGTGTTCATACTGGCTTGGATCACAAGGAGATCCA
CCGTACCAGTAAGTTGGTAAGTCAGCTTTGCCACATGCCAATTCAAGACAACAAAGCAATCGTCGGTGCTAACGCATTCA
GCCACTCTTCAGGTATTCACCAAGATGGCATGCTAAAGAACAAGAACACTTACGAGATCATGACGCCTGAGTCTATTGGC
TTGAAAAACAAAGCCTTAAACCTAACAAGCCGTAGTGGCCGAGCAGCGGTTAAGAGCCACATGGACGCGATGGGTTATAA
AGACAATGAGTACAACCTAGATTCATTGTACGAAGACTTCTTGAAGCTTGCTGATCGTAAAGGACAAGTGTTCGATTACG
ACCTAGAAGCATTGATGCACTTCGCGAATCTACGTGATGAAGATGACTTCTATAAACTTAACTACCTAAGCGTACAATCT
GGTAGTGTTATGTCTACCACCAGCATCAAACTGCAATGTGGTGATGAAGAGAAATGCGAAGCGGCTGTCGGCAATGGCCC
GGTTGATGCTTTATACCAATGTATCTACCGCTTAACAGGCTACGAAATCGCGTTGGATAAGTTCGACCTAACTGCAAAAG
GTGAAGGTGAAGATGGCTTAGGTCAAGCTGACATCATTGCTAACTATAAAGGCCGTAAGTACCACGGTACGGGCGTTTCA
ACCGATATCGTTGAAGCTTCTGGTCAAGCGTTGCTACACGTTATCAATAGCATTCAACGCGCAGACACCATTGCTGAAAT
GAAGCAGCAAAAATTCGCGACAGTTTAA

Upstream 100 bases:

>100_bases
CACTGAGCGGGTTTTTTTGTAACTGGCACTTTTAAAAGAACATCATTCTTAATAATAATTTGGATAGCAATCGATTCATT
ATCGATATAAGGAAGCACCC

Downstream 100 bases:

>100_bases
TACCCCAATAACAGAGCCCTAGAGCCGATGTTGTTGGCTTTAGGGACAAAATTTAAAACGAGCCAAACAGCTCAGTAACA
AGAATTAAAGGATTAACATG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNDQVIIFDTTLRDGEQALAASLTVKEKLQIAYALERLGVDVIEAGFPISSPGDFESVQTIAKHIKDSRICALSRAVAKD
IDAAAEALKVADQFRIHTFISTSTVHVQDKLRRSYDDVVEMAVKAVKHARNYTDDVEFSCEDAGRTPIDNLCRMVEAAIN
AGAKTINIPDTVGYTVPNEFGGIIKTLFDRVPNIDQAIISVHCHDDLGMSVANSIAAVQAGARQIEGTINGIGERAGNCS
LEEIAMIIKTRQELLGVHTGLDHKEIHRTSKLVSQLCHMPIQDNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNKALNLTSRSGRAAVKSHMDAMGYKDNEYNLDSLYEDFLKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMSTTSIKLQCGDEEKCEAAVGNGPVDALYQCIYRLTGYEIALDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS
TDIVEASGQALLHVINSIQRADTIAEMKQQKFATV

Sequences:

>Translated_515_residues
MNDQVIIFDTTLRDGEQALAASLTVKEKLQIAYALERLGVDVIEAGFPISSPGDFESVQTIAKHIKDSRICALSRAVAKD
IDAAAEALKVADQFRIHTFISTSTVHVQDKLRRSYDDVVEMAVKAVKHARNYTDDVEFSCEDAGRTPIDNLCRMVEAAIN
AGAKTINIPDTVGYTVPNEFGGIIKTLFDRVPNIDQAIISVHCHDDLGMSVANSIAAVQAGARQIEGTINGIGERAGNCS
LEEIAMIIKTRQELLGVHTGLDHKEIHRTSKLVSQLCHMPIQDNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNKALNLTSRSGRAAVKSHMDAMGYKDNEYNLDSLYEDFLKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMSTTSIKLQCGDEEKCEAAVGNGPVDALYQCIYRLTGYEIALDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS
TDIVEASGQALLHVINSIQRADTIAEMKQQKFATV
>Mature_515_residues
MNDQVIIFDTTLRDGEQALAASLTVKEKLQIAYALERLGVDVIEAGFPISSPGDFESVQTIAKHIKDSRICALSRAVAKD
IDAAAEALKVADQFRIHTFISTSTVHVQDKLRRSYDDVVEMAVKAVKHARNYTDDVEFSCEDAGRTPIDNLCRMVEAAIN
AGAKTINIPDTVGYTVPNEFGGIIKTLFDRVPNIDQAIISVHCHDDLGMSVANSIAAVQAGARQIEGTINGIGERAGNCS
LEEIAMIIKTRQELLGVHTGLDHKEIHRTSKLVSQLCHMPIQDNKAIVGANAFSHSSGIHQDGMLKNKNTYEIMTPESIG
LKNKALNLTSRSGRAAVKSHMDAMGYKDNEYNLDSLYEDFLKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQS
GSVMSTTSIKLQCGDEEKCEAAVGNGPVDALYQCIYRLTGYEIALDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS
TDIVEASGQALLHVINSIQRADTIAEMKQQKFATV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=512, Percent_Identity=68.359375, Blast_Score=743, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=555, Percent_Identity=25.045045045045, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=543, Percent_Identity=25.7826887661142, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=362, Percent_Identity=28.1767955801105, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=28.5318559556787, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56388; Mature: 56388

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQVIIFDTTLRDGEQALAASLTVKEKLQIAYALERLGVDVIEAGFPISSPGDFESVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAKHIKDSRICALSRAVAKDIDAAAEALKVADQFRIHTFISTSTVHVQDKLRRSYDDVVE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEHHHHHHHHHHHHHH
MAVKAVKHARNYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGAKTINIPDTVGYTVPNEF
HHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIIKTLFDRVPNIDQAIISVHCHDDLGMSVANSIAAVQAGARQIEGTINGIGERAGNCS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEIAMIIKTRQELLGVHTGLDHKEIHRTSKLVSQLCHMPIQDNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGMLKNKNTYEIMTPESIGLKNKALNLTSRSGRAAVKSHMDAMGYKDNEYNLDSLYEDF
CCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMSTTSIKLQCGDEEKCE
HHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHH
AAVGNGPVDALYQCIYRLTGYEIALDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS
HHCCCCCHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCEEECCCCCEEECCCCC
TDIVEASGQALLHVINSIQRADTIAEMKQQKFATV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDQVIIFDTTLRDGEQALAASLTVKEKLQIAYALERLGVDVIEAGFPISSPGDFESVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAKHIKDSRICALSRAVAKDIDAAAEALKVADQFRIHTFISTSTVHVQDKLRRSYDDVVE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEHHHHHHHHHHHHHH
MAVKAVKHARNYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGAKTINIPDTVGYTVPNEF
HHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIIKTLFDRVPNIDQAIISVHCHDDLGMSVANSIAAVQAGARQIEGTINGIGERAGNCS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEIAMIIKTRQELLGVHTGLDHKEIHRTSKLVSQLCHMPIQDNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGMLKNKNTYEIMTPESIGLKNKALNLTSRSGRAAVKSHMDAMGYKDNEYNLDSLYEDF
CCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADRKGQVFDYDLEALMHFANLRDEDDFYKLNYLSVQSGSVMSTTSIKLQCGDEEKCE
HHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHH
AAVGNGPVDALYQCIYRLTGYEIALDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS
HHCCCCCHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCEEECCCCCEEECCCCC
TDIVEASGQALLHVINSIQRADTIAEMKQQKFATV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]