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The map label for this gene is yfhD

Identifier: 218701071

GI number: 218701071

Start: 2870458

End: 2871876

Strand: Direct

Name: yfhD

Synonym: ECIAI39_2762

Alternate gene names: 218701071

Gene position: 2870458-2871876 (Clockwise)

Preceding gene: 218701065

Following gene: 218701074

Centisome position: 55.93

GC content: 50.25

Gene sequence:

>1419_bases
GTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACA
GTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTCGATAATG
GTAACGCCGACCTGCTGGCGGCAGGCCTGGTCTATAACAGTGAGCGTGTAAAAAATTATCAGCCTGGCCCTACCTATTAT
TCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACACTGGGCAACCTGACGGCGGAGCAACTCAC
CGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTTG
ACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTTGCC
ATTAGCCTGTTTCAGCGCGTTCATCCGGAACTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACCTGGTTTAG
CCCGTTAGATGGCGATAATACCCTTTCCGCCGCTCTGCTCGATTTCTTCAATGAGATGAATGAAGACGGTACGCTGGCAC
GCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCG
GTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCA
GGAATCGCACTGGGATGCGCAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGCTAACCAAAAATACCGCGCAAA
GCCTCGGCATTACGGATCGTACCGATGCCGAACAGAGCATCAGCGGCGGCGTGCGTTATTTGCAGGATATGATGAGTAAA
GTGCCGGAAAGTGTGCCGGAGAACGAACGGATTTGGTTTGCCCTCGCCGCGTACAATATGGGCTATGCGCATATGCTGGA
TGCCCGCGCTCTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCC
AGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGGCATGAAGCCTACGCTTATGTCGAAAATATTCGTAAG
TATCAGATTAGCCTGGTGGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGCTTA
TCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATT
TGACCCACTCTCCCTCTCTGCTATTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAA

Upstream 100 bases:

>100_bases
TCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTC
AAGCGCGGGGAGAGTTGCGT

Downstream 100 bases:

>100_bases
TCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCA
CTCATCCGCCAGTATTCCTT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYY
SVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVA
ISLFQRVHPELAVALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDA
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSK
VPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRK
YQISLVGYLQEKEKQATEAAMQLAQAYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Sequences:

>Translated_472_residues
MSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYY
SVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVA
ISLFQRVHPELAVALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDA
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSK
VPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRK
YQISLVGYLQEKEKQATEAAMQLAQAYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
>Mature_471_residues
STIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYS
VSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAI
SLFQRVHPELAVALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAV
LPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKV
PESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKY
QISLVGYLQEKEKQATEAAMQLAQAYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=472, Percent_Identity=99.5762711864407, Blast_Score=969, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 53114; Mature: 52983

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLA
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEE
AGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQT
EHHEECHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHH
LKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDE
HHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCC
QPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDA
CCCEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHCCCCCC
TDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHEECCCCCCC
SWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAA
HHHHHHHHCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQLAQAYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
HHHHHHCCCCCHHHCCCHHCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC
>Mature Secondary Structure 
STIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLA
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEE
AGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQT
EHHEECHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHH
LKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDE
HHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCC
QPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDA
CCCEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHCCCCCC
TDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHEECCCCCCC
SWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAA
HHHHHHHHCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQLAQAYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
HHHHHHCCCCCHHHCCCHHCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA