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The map label for this gene is hyfJ

Identifier: 218700948

GI number: 218700948

Start: 2728229

End: 2728642

Strand: Direct

Name: hyfJ

Synonym: ECIAI39_2629

Alternate gene names: 218700948

Gene position: 2728229-2728642 (Clockwise)

Preceding gene: 218700947

Following gene: 218700949

Centisome position: 53.16

GC content: 53.14

Gene sequence:

>414_bases
ATGACTGAAGAGTGCGGGGAAATTGTTTTCTGGACGCTGCGAAAAAAGTTTGTCGCCAGTAGCGACGAGATGCCGGAACA
CAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCT
GCCCACAGACGGAATACGAAGATTGGCTTGCACAGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACC
TTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCAGATGACGCGACGTC
GGCGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGCCA
GAAAAATTGCGTGA

Upstream 100 bases:

>100_bases
GATCCTACAGGAAATCGGGTTAACACCTGGTTGCGCGATGCCGACGATCCACGTCTCAATAGTATCGTTCAGCAACTCTT
TCGCGTACTCCGGGGGTTAC

Downstream 100 bases:

>100_bases
GAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCGCAAGGAATAACAATT
GAAGCGGTAAACGGAATGCT

Product: putative processing element hydrogenase 4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGVIDCLNVAFRCPQTEYEDWLAQVEEEQARRKMLGVMT
FGEIVIDASHTALLTRAFAPLADDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA

Sequences:

>Translated_137_residues
MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGVIDCLNVAFRCPQTEYEDWLAQVEEEQARRKMLGVMT
FGEIVIDASHTALLTRAFAPLADDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA
>Mature_136_residues
TEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGVIDCLNVAFRCPQTEYEDWLAQVEEEQARRKMLGVMTF
GEIVIDASHTALLTRAFAPLADDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli hycH [H]

Homologues:

Organism=Escherichia coli, GI87082116, Length=137, Percent_Identity=97.8102189781022, Blast_Score=278, Evalue=7e-77,
Organism=Escherichia coli, GI1789073, Length=131, Percent_Identity=48.0916030534351, Blast_Score=122, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010005
- ProDom:   PD021295 [H]

Pfam domain/function: PF07450 HycH [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 15579; Mature: 15448

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGVIDCLNVAFRCPQTEYE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCCHHH
DWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLADDATSAWQARSIQFIHLL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DEIVQEPAIYLMARKIA
HHHHHCHHHHHHHHHCC
>Mature Secondary Structure 
TEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGVIDCLNVAFRCPQTEYE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCCHHH
DWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLADDATSAWQARSIQFIHLL
HHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DEIVQEPAIYLMARKIA
HHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]