Click here to switch to the map view.

The map label for this gene is hyfI

Identifier: 218700947

GI number: 218700947

Start: 2727478

End: 2728236

Strand: Direct

Name: hyfI

Synonym: ECIAI39_2628

Alternate gene names: 218700947

Gene position: 2727478-2728236 (Clockwise)

Preceding gene: 218700946

Following gene: 218700948

Centisome position: 53.15

GC content: 55.73

Gene sequence:

>759_bases
ATGAGTCCAGTGCTTACGCAACATGTCAGCCAGCCCATTACGCTGGACGAGCAAACGCAAAAGATGAAGCAGCATTTGCT
ACAGGATATCCGTCGCTCGGCTTACGTTTATCGCGTCGATTGCGGCGGCTGTAACGCCTGCGAAATCGAAATTTTTGCCG
CCATCACACCCGTATTCGACGCAGAACGTTTTGGCATTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTT
ACTGGCGCAGTCACCCGTGCGATGCGTATGCCTGCACTTCGGGCATATGAGTCTGCCCCCGATCATAAAATTTGTGTTTC
CTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTTTACAGCGTCTGGGGCGGCAGCGACACCATTGTCCCCA
TTGATGTTTGGATCCCCGGCTGCCCGCCAACACCGGCCGCCACCATTCACGGTTTCGCCGTGGCGCTCGGTTTGCTGCAA
CAGAAGATTCACGCGGTGGATTATCGCGATCCCACCGGGGTGACCATGCAACCGCTGTGGCCGCAGATCCCGCCATCACA
GCGTATCGCCATTGAGCGAGAAGCGAGGCGGCTGGCGGGCTATCGTCAAGGGCGAGAAATTTGCGATCGACTCCTGCGCC
ATTTAAGCGACGATCCTACAGGAAATCGGGTTAACACCTGGTTGCGCGATGCCGACGATCCACGTCTCAATAGTATCGTT
CAGCAACTCTTTCGCGTACTCCGGGGGTTACATGACTGA

Upstream 100 bases:

>100_bases
AAATGTTACGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGACACAAATGAACCG
CTGGTGGCTAAGGAGCAGCT

Downstream 100 bases:

>100_bases
AGAGTGCGGGGAAATTGTTTTCTGGACGCTGCGAAAAAAGTTTGTCGCCAGTAGCGACGAGATGCCGGAACACAGCTCTC
AGGTAATGTATTACTCGCTA

Product: hydrogenase 4, Fe-S subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERFGIKVVSSPRHADILLF
TGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
QQLFRVLRGLHD

Sequences:

>Translated_252_residues
MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERFGIKVVSSPRHADILLF
TGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
QQLFRVLRGLHD
>Mature_251_residues
SPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFT
GAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQQ
KIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIVQ
QLFRVLRGLHD

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=123, Percent_Identity=41.4634146341463, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788834, Length=252, Percent_Identity=99.6031746031746, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1789074, Length=251, Percent_Identity=62.1513944223108, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1788624, Length=161, Percent_Identity=33.5403726708075, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=122, Percent_Identity=37.7049180327869, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=40, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=40, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24651058, Length=123, Percent_Identity=39.0243902439024, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28074; Mature: 27942

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFD
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCHHC
AERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHD
HHHHCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQQKIHAVDYRDPTGVTMQPLW
HHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCC
PQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHH
QQLFRVLRGLHD
HHHHHHHHHCCC
>Mature Secondary Structure 
SPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGCNACEIEIFAAITPVFD
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCHHC
AERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDHKICVSYGACGVGGGIFHD
HHHHCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQQKIHAVDYRDPTGVTMQPLW
HHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCC
PQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDDPTGNRVNTWLRDADDPRLNSIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHH
QQLFRVLRGLHD
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]