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The map label for this gene is insK [C]

Identifier: 218700828

GI number: 218700828

Start: 2590749

End: 2591639

Strand: Direct

Name: insK [C]

Synonym: ECIAI39_2503

Alternate gene names: 218700828

Gene position: 2590749-2591639 (Clockwise)

Preceding gene: 218700827

Following gene: 218700831

Centisome position: 50.48

GC content: 54.21

Gene sequence:

>891_bases
ATGATGCCACTGCTGGATAAGCTGCGTGAGCAGTACGGGGTCGGACCGCTATGCAGCGAACTGCATATTGCCCCGTCAAC
GTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAG
AGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATC
AGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAC
GACCATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGG
TGGCTGATTTTACTTACGTCAGCACATGGCGGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATC
GTGGGGTGGCGGGTCTCATCGTCCATGGAAACGACATTCGTGCTGGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACC
GTCCGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGCCTACACACAGCGGCTTAAGGAAGCCGGAT
TACTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAG
GTAATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTCACGTGGGTGGACTGGTATAACAATCG
ACGATTGCTGGAAAGGCTGGGCCATACTCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATC
TGGCAGCCTGA

Upstream 100 bases:

>100_bases
AGCTGGAACGTGAAAATCGTGAACTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAG
TTCGACCGCCTCTGGAAAAA

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCAATCCCCTCCCGTGCTGGTAATCTGAGACTGTGCGCCTT
CAACAAATAAAGCAACACCA

Product: transposase ORF B, IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_296_residues
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA
>Mature_296_residues
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=260, Percent_Identity=50.3846153846154, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI1789981, Length=286, Percent_Identity=30.0699300699301, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788405, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787263, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786753, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786570, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786490, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33809; Mature: 33809

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYD
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
ALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
HHHHCCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA
HHHHHHCCCCCCEEEEEEEEEHHHCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYD
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
ALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
HHHHCCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA
HHHHHHCCCCCCEEEEEEEEEHHHCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]