Click here to switch to the map view.

The map label for this gene is flk

Identifier: 218700797

GI number: 218700797

Start: 2558290

End: 2559285

Strand: Direct

Name: flk

Synonym: ECIAI39_2470

Alternate gene names: 218700797

Gene position: 2558290-2559285 (Clockwise)

Preceding gene: 218700778

Following gene: 218700800

Centisome position: 49.85

GC content: 51.81

Gene sequence:

>996_bases
ATGATACAACCTATTTCCGGCCCTCCTCCTGGGCAACCACCAGGTCAGGGAGATAACCTGCCGTCTGGCGCGGGCAATCA
GCCTTTATCCAGTCAGCAACGTACTTCGCTGGAAAGCTTAATGACGAAAGTGACCTCACTGACGCAACAGCAAAGAGCAG
AACTGTGGGCGGGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAG
CATAATCTGGCGCAACGTCTGTTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTT
ACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTATATCCGTCATAACTTTGGTCAGACGCCGCTGAATCAGCTCTCAC
CGGAGCAATTAAAAACCATTCTCACCCTGTTGCAGGAAGGGAAAATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACC
GACCGTCCTTTATTACCGGCGGAGCACAATGCGCTCAAACAGTTGGTGACCAAACTTGCGGCAGCAACGGGGGAACCCAG
CAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATC
TGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACACTGGAATCACTACAGATGGCGCTAAAA
CAACCTTTAGATGCCAGTGAACTGGCGGCGTTATCGACATATATCCAACAAAAATATGGCCTTTCTGCGCAATCATCGCT
TTCTTCTTCCCAGGCCGAGGATATTCTTAACCAGCTTTATCAACGGCGGGTTAAAGGGATTGAACCGCGTGATATGCAAC
CGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTC
GCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAA

Upstream 100 bases:

>100_bases
ACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGAT
TTCAGGTATAAGGATACGTA

Downstream 100 bases:

>100_bases
CCCCGACGAAACGACAGTATCGTGACAATAATTCCCAGCACCGCAGAGATCGCCCCGGCAAGAAATACCGAAGAGTAACC
AAACGTGGTCGCCAGCATTC

Product: flagella biosynthesis regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKVTSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAE
HNLAQRLLAAQKSHSARQLLAQLGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWLQARQTLSQQNTPTLESLQMALK
QPLDASELAALSTYIQQKYGLSAQSSLSSSQAEDILNQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLV
AIILMLVWLVR

Sequences:

>Translated_331_residues
MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKVTSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAE
HNLAQRLLAAQKSHSARQLLAQLGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWLQARQTLSQQNTPTLESLQMALK
QPLDASELAALSTYIQQKYGLSAQSSLSSSQAEDILNQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLV
AIILMLVWLVR
>Mature_331_residues
MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKVTSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAE
HNLAQRLLAAQKSHSARQLLAQLGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWLQARQTLSQQNTPTLESLQMALK
QPLDASELAALSTYIQQKYGLSAQSSLSSSQAEDILNQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLV
AIILMLVWLVR

Specific function: Acts as a regulator of flagellar gene expression by modulating the protein level of the anti sigma factor flgM upon sensing ring completion or hook elongation. Flk could inhibit flgM secretion by acting as a braking system for the flagellar- associated ty

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flk family [H]

Homologues:

Organism=Escherichia coli, GI1788661, Length=331, Percent_Identity=98.1873111782477, Blast_Score=648, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36685; Mature: 36685

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKVTSLTQQQRAELWAGIR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQLGEYLRLGNNRQAVTDYI
HHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREATDRPLLPAEHNALKQLVTKLA
HHCCCCCCHHHCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWLQARQTLSQQNTPTLESLQMALK
HHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QPLDASELAALSTYIQQKYGLSAQSSLSSSQAEDILNQLYQRRVKGIEPRDMQPLLNPFP
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCC
PMMDTLQNMATRPALWILLVAIILMLVWLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKVTSLTQQQRAELWAGIR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQLGEYLRLGNNRQAVTDYI
HHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREATDRPLLPAEHNALKQLVTKLA
HHCCCCCCHHHCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWLQARQTLSQQNTPTLESLQMALK
HHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QPLDASELAALSTYIQQKYGLSAQSSLSSSQAEDILNQLYQRRVKGIEPRDMQPLLNPFP
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCC
PMMDTLQNMATRPALWILLVAIILMLVWLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2681152 [H]