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The map label for this gene is mnmC

Identifier: 218700800

GI number: 218700800

Start: 2562104

End: 2564110

Strand: Direct

Name: mnmC

Synonym: ECIAI39_2473

Alternate gene names: 218700800

Gene position: 2562104-2564110 (Clockwise)

Preceding gene: 218700797

Following gene: 218700807

Centisome position: 49.92

GC content: 51.52

Gene sequence:

>2007_bases
GTGAAACACTACGCCATACAACCTGCCAACCTTGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGT
CTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTCGGGGGTAACCATTTAGAGGCACGTTTTCCTG
AACATCCACATCCTCTGTTTGTGGTAGCAGAGAGTGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTT
GATCAGTTTCGCGAAGCACATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCGCCCG
TGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCGGAACAACTTCAGGCACAATGGCCTTTGC
CCCTGTCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTAACGCTGGATTTATGGTTTGGCGATATAAACGAACTG
ACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGA
TATGTGGACGCAAAATCTGTTTAAGGCCATGGCAAGGTTAGCGCGTCCGGGCGGCACGCTGGCGACCTTTACGTCTGCCG
GTTTTGTCCGCCGCGGTTTGCAGGAAGCCGGATTCACCATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTT
TGCGGGGTGATGGAACAGACATTACCCCTCCCCTGCTCCACGCCGTGGTTTAACCGTACGGGCAGCAACAAACAGGAAGC
GGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAAGTAACGCTTTATT
GCGCGGATGAGGCTCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGCTTTTAAGTAAACACGATGAA
GCGCTAAACCGTTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGATTTATTGCCCGTTAAATTTGATCATGA
CTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCGATGAATTTACCCG
CAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGTATTACTTAT
CCGCAAGGTGGCTGGCTGTGTCCGGCAGAACTAACCCGTAATGTGCTGGAACTGGCACAACAGCAAGGTTTGCAAATTCG
TTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGAATTTTGCAGGTGATCAGCAAGCAACAC
ACAGTGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGTTTCAGCCAAACGTCGTCTCTGCCAGTATATTCGGTGGCC
GGTCAGGTCAGCCATATTCCGACAACGCCGGAACTGGCAAAACTGAAACAGGTGCTGTGCTATGACGGTTATCTCACGCC
ACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACG
ATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAATGGGCGAAAGCGGTTGATGTCAGTGATAAA
GAGGCGCGTTGCGGTGTACGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAACGTGCCTGATTATGACGCTACACT
CGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATGTTTGCTG
CTTTAGGTTCGCGCGGTTTGTGTTCTGCTCCGCTGTGTGCCGAGATTCTGGCGGCACAGATGAGTGAAGAACCGATTCCG
ATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAGTTGTTGAAGGGTAAAGCGGTTAAGGC
AGGGTAA

Upstream 100 bases:

>100_bases
GTACGCCGAACAAGTCCGATCAGCCAATTAATAGAGAAATTTGCGCAGCCTGGCACACATCGCTAAGATCGAGCCACCGC
CTGTAAGACGAGTAACTTAC

Downstream 100 bases:

>100_bases
TCTGCTCTGGCATTGCTTGCCGGATATGGCGTGAACGCCGCATCCGGCACATTACTTACTGCTGTGACGCCTGCTGAAAC
AGGTGTTCCCACATATCGGT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQAQLQRLHFISFEKFPLARADLALAHQHWPELAPWAEQLQAQWPLPLSGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMNLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIRYQHQLQDLSRKDDGWLLNFAGDQQATHSVVVLANGHQISRFSQTSSLPVYSVA
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKAVDVSDK
EARCGVRCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAPLCAEILAAQMSEEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG

Sequences:

>Translated_668_residues
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQAQLQRLHFISFEKFPLARADLALAHQHWPELAPWAEQLQAQWPLPLSGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMNLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIRYQHQLQDLSRKDDGWLLNFAGDQQATHSVVVLANGHQISRFSQTSSLPVYSVA
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKAVDVSDK
EARCGVRCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAPLCAEILAAQMSEEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG
>Mature_668_residues
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQAQLQRLHFISFEKFPLARADLALAHQHWPELAPWAEQLQAQWPLPLSGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMNLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIRYQHQLQDLSRKDDGWLLNFAGDQQATHSVVVLANGHQISRFSQTSSLPVYSVA
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKAVDVSDK
EARCGVRCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAPLCAEILAAQMSEEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=96.4071856287425, Blast_Score=1323, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 74248; Mature: 74248

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNHLEARFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEECCCCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEKFPLARADLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
APWAEQLQAQWPLPLSGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEECCC
APAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
CGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDLLPVKFDHDWCGVTQLGWDEKS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCHH
QHKIAQMLSMNLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCEECHHHHHHHHHHHHH
QQGLQIRYQHQLQDLSRKDDGWLLNFAGDQQATHSVVVLANGHQISRFSQTSSLPVYSVA
HCCCEEEEHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCEEHHHHCCCCCCEEECC
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
RLIDCFPQAQWAKAVDVSDKEARCGVRCATRDHLPMVGNVPDYDATLVEYASLAEKKDEA
HHHHHCCCHHHHHHCCCCCCHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VSAPVYDDLFMFAALGSRGLCSAPLCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC
>Mature Secondary Structure
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNHLEARFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEECCCCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEKFPLARADLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
APWAEQLQAQWPLPLSGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEECCC
APAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
CGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDLLPVKFDHDWCGVTQLGWDEKS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCHH
QHKIAQMLSMNLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCEECHHHHHHHHHHHHH
QQGLQIRYQHQLQDLSRKDDGWLLNFAGDQQATHSVVVLANGHQISRFSQTSSLPVYSVA
HCCCEEEEHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCEEHHHHCCCCCCEEECC
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
RLIDCFPQAQWAKAVDVSDKEARCGVRCATRDHLPMVGNVPDYDATLVEYASLAEKKDEA
HHHHHCCCHHHHHHCCCCCCHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VSAPVYDDLFMFAALGSRGLCSAPLCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA