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The map label for this gene is spr

Identifier: 218700647

GI number: 218700647

Start: 2384134

End: 2384700

Strand: Direct

Name: spr

Synonym: ECIAI39_2316

Alternate gene names: 218700647

Gene position: 2384134-2384700 (Clockwise)

Preceding gene: 218700646

Following gene: 218700648

Centisome position: 46.46

GC content: 47.27

Gene sequence:

>567_bases
ATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATCCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATG
TAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTC
AGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGGATTATGGATCAGTATGCTGACTGGAAAGGCGTA
CGCTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGTACATTCCGTGAGCAATTTGG
CTTAGAACTTCCGCGTTCGACTTATGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATT
TAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCGGTATTTATATCGGCAACAACCAGTTTGTCCATGCTTCCACC
AGCAGTGGCGTTATTATTTCCAGCATGAATGAACCATACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCG
CAGCTAA

Upstream 100 bases:

>100_bases
AAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGAC
TTCAAGGGAAAACAAACAAC

Downstream 100 bases:

>100_bases
TAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCA
TTCATCAGAGAAAATGATGT

Product: putative outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVGSETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGV
RYRLGGSTKKGIDCSGFVQRTFREQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
SSGVIISSMNEPYWKKRYNEARRVLSRS

Sequences:

>Translated_188_residues
MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVGSETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGV
RYRLGGSTKKGIDCSGFVQRTFREQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
SSGVIISSMNEPYWKKRYNEARRVLSRS
>Mature_188_residues
MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVGSETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGV
RYRLGGSTKKGIDCSGFVQRTFREQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
SSGVIISSMNEPYWKKRYNEARRVLSRS

Specific function: Unknown

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family

Homologues:

Organism=Escherichia coli, GI1788501, Length=188, Percent_Identity=100, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1788001, Length=127, Percent_Identity=48.0314960629921, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787944, Length=122, Percent_Identity=36.0655737704918, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786421, Length=114, Percent_Identity=34.2105263157895, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPR_ECO57 (P0AFV6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91012
- PIR:   E85856
- RefSeq:   NP_288758.1
- RefSeq:   NP_311094.1
- ProteinModelPortal:   P0AFV6
- SMR:   P0AFV6
- EnsemblBacteria:   EBESCT00000026997
- EnsemblBacteria:   EBESCT00000059080
- GeneID:   916771
- GeneID:   957128
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3434
- KEGG:   ecs:ECs3067
- GeneTree:   EBGT00050000009578
- HOGENOM:   HBG362075
- OMA:   GRHIGIY
- ProtClustDB:   PRK10838
- BioCyc:   ECOL83334:ECS3067-MONOMER
- InterPro:   IPR000064

Pfam domain/function: PF00877 NLPC_P60

EC number: NA

Molecular weight: Translated: 21040; Mature: 21040

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVGSETSSLQASQDEFENL
CCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHCCCCHHCCCCHHHHHHH
VRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTFREQFGLELPRSTYEQQEM
HHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
GKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHASTSSGVIISSMNEPYWKKRYNE
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCHHHHHHHH
ARRVLSRS
HHHHHCCC
>Mature Secondary Structure
MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVGSETSSLQASQDEFENL
CCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHCCCCHHCCCCHHHHHHH
VRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTFREQFGLELPRSTYEQQEM
HHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
GKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHASTSSGVIISSMNEPYWKKRYNE
HHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCHHHHHHHH
ARRVLSRS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796