Click here to switch to the map view.

The map label for this gene is yeiT

Identifier: 218700618

GI number: 218700618

Start: 2348433

End: 2349671

Strand: Direct

Name: yeiT

Synonym: ECIAI39_2285

Alternate gene names: 218700618

Gene position: 2348433-2349671 (Clockwise)

Preceding gene: 218700617

Following gene: 218700619

Centisome position: 45.76

GC content: 50.36

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGCCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTT
ATTATGTCATGACGCTCCCTGTAGTCAGGCTTGCCCGGCGCAAACTGACCCAGGGAAATTTATCCGCTCAATTTATTTTC
GTAATTTTAAAGGTGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAG
AAATTATGCCAAAGCGGTTGTACACGTGCCGGTGTTGATACTCCTATTGATATCGGTCGCTTACAGCGTTTTGTTACTGA
TTTTGAACAACAAACCGGGATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCGGGTC
CTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATCTATGAGAAAGAAGCACAACCCGGT
GGCTGGTTGCGTAACGGCATTCCGCAATTCCGGTTACCGCAATCAGTACTGGATGCAGAAATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAATAACGAAATCGGTAAAACACTCACCCTGGAGCAGCTGAAAGCGGAAAACCGCGCGGTAC
TGGTCACTGTGGGGTTATCGAGCGGTTCTGGGTTATCGCTGTTTGAGCATAGCGACGTTGAGATTGCCGTCGACTTCCTG
CAACGTGCACGACAGGCGCAAGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTCGCGATGGA
CGTAGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCAGTAACTTGCGTAGCGCGTGAAGAGTTAGATGAGTTTCCGGCAA
GCGAAAAAGAGTTTGCCAGCGCCCGGGAGTTGGGCGTTTCGATCATCGATGGATTTACACCTGTAGCCGTGGAAGGCAAT
AAAGTCACCTTTAAGCATGTTCGTTTACCAGGTGAACTAACGATAGCGGCGGACAAAATTATTCTCGCTGTAGGTCAACA
TGCCGAACTTGCTGCTTTTTCCGCACTGGAACCGCAGCGTAACACCATCGAAACGCAACATTACCAGACCCGCGACCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
CTTTCACAGTTTAAATTTCCCCACGCGCATAGCCTTAATATCAGCACGCAATATTTATTTAGCGTTCGCCTTAATTACTC
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGC
TATGAGATGTGTGCCAAAGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLPGELTIAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLPGELTIAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGG
WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFLQ
RARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNK
VTFKHVRLPGELTIAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=31.8077803203661, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=48.8888888888889, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=96.3592233009709, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=449, Percent_Identity=32.0712694877506, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=31.6742081447964, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=29.8440979955457, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789243, Length=398, Percent_Identity=29.6482412060301, Blast_Score=141, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=33.7868480725624, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=28.0623608017817, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=25.3796095444685, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=31.3364055299539, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=31.3364055299539, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24665547, Length=443, Percent_Identity=27.0880361173815, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24665543, Length=443, Percent_Identity=27.0880361173815, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28574881, Length=447, Percent_Identity=27.7404921700224, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24665539, Length=447, Percent_Identity=27.7404921700224, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44252; Mature: 44121

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELTIAADKIILAVGQHAELAAFSALEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEECCCCEEEEECEEEEEECCCCHHHHHHCCCCCC
NTIETQHYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELTIAADKIILAVGQHAELAAFSALEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEECCCCEEEEECEEEEEECCCCHHHHHHCCCCCC
NTIETQHYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]