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The map label for this gene is rutA

Identifier: 218700477

GI number: 218700477

Start: 2189825

End: 2190820

Strand: Direct

Name: rutA

Synonym: ECIAI39_2143

Alternate gene names: 218700477

Gene position: 2189825-2190820 (Clockwise)

Preceding gene: 218700475

Following gene: 218700478

Centisome position: 42.67

GC content: 54.82

Gene sequence:

>996_bases
TTGCAAACAAGCCATTACGCGGCTGAAAAGGATATGCAGGATGCAGCGCCCCGTCTGACTTTCACTTTACGAGACGAAGA
GAGGTTGATGATGAAAATTGGCGTATTCGTACCTATTGGCAACAACGGCTGGCTCATTTCGACCCACGCGCCGCAGTACA
TGCCGACCTTTGAACTGAATAAAGCCATTGTGCAAAAAGCGGAGCACTACCATTTCGACTTCGCCCTGTCGATGATCAAA
CTGCGTGGCTTTGGCGGCAAAACTGAGTTCTGGGATCACAACCTTGAGTCGTTCACCTTGATGGCGGGGCTGGCAGCCGT
GACCTCGCGGATTCAGATTTACGCCACTGCCGCCACCTTAACGTTACCTCCGGCAATCGTCGCCCGTATGGCCGCAACCA
TTGACTCCATCTCTGGCGGGCGTTTTGGCGTCAACCTCGTGACTGGCTGGCAAAAGCCCGAGTATGAGCAGATGGGGATC
TGGCCTGGCGATGACTATTTCTCCCGTCGTTACGACTATCTCACCGAATATGTTCAGGTGCTGCGCGACCTGTGGGGCAC
GGGCAAAAGCGATTTTAAAGGCGATTTTTTCACCATGAATGATTGTCGCGTCAGTCCGCAACCGAGCGTACCCATGAAAG
TGATCTGCGCCGGGCAAAGCGACGCTGGCATGGCGTTCTCCGCCCAGTATGCCGATTTTAACTTCTGTTTCGGCAAAGGC
GTAAATACACCCACGGCTTTCGCCCCGACCGCTGCGCGGATGAAACAGGCCGCAGAGCAAACCGGGCGCGACGTTGGCTC
TTATGTGTTGTTTATGGTGATTGCCGACGAAACCGACGATGCCGCTCGCACCAAATGGGAACACTACAAAGCGGGCGCGG
ATGAAGAGGCGTTAAGCTGGCTAACCGAACAAAGTCAGAAAGATACCCGCTCCGGTACAGACACCAACGTCCGTCAGATG
GCCGATCCCACTTCGGCGAGCGCATTCAACCACTGA

Upstream 100 bases:

>100_bases
GCAACTGTTTTGACCGTTTAGTCCACTTTTTTCCAGATATTTCAGACAACCAAACATTAACACATTAATAACATTCGCTT
TTTAAAAACTGGCATCCGCT

Downstream 100 bases:

>100_bases
TGCAGTGTCGCGCCCATCTCCCTGCGCTGACTCAGGAGGTGGCATGATGACAACCTTAACCGCACGACCGGAAGCCATTA
CCTTCGATCCGCAGCAAACT

Product: alternative pyrimidine degradation pathway monooxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQTSHYAAEKDMQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARTKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSASAFNH

Sequences:

>Translated_331_residues
MQTSHYAAEKDMQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARTKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSASAFNH
>Mature_331_residues
MQTSHYAAEKDMQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARTKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSASAFNH

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787247, Length=319, Percent_Identity=98.4326018808777, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI1787166, Length=211, Percent_Identity=26.5402843601896, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: NA

Molecular weight: Translated: 36907; Mature: 36907

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSHYAAEKDMQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELN
CCCCCHHHHHHHHHCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHH
KAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEE
TLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQV
ECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
HHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCC
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARTKWEHYKAGADEEALSW
CCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
LTEQSQKDTRSGTDTNVRQMADPTSASAFNH
HHHHHHHHHCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQTSHYAAEKDMQDAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELN
CCCCCHHHHHHHHHCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHH
KAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEE
TLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQV
ECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
HHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCC
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARTKWEHYKAGADEEALSW
CCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
LTEQSQKDTRSGTDTNVRQMADPTSASAFNH
HHHHHHHHHCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA