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The map label for this gene is mexA [H]

Identifier: 218700004

GI number: 218700004

Start: 1703116

End: 1704267

Strand: Direct

Name: mexA [H]

Synonym: ECIAI39_1633

Alternate gene names: 218700004

Gene position: 1703116-1704267 (Clockwise)

Preceding gene: 218700003

Following gene: 218700005

Centisome position: 33.19

GC content: 50.87

Gene sequence:

>1152_bases
ATGAGGAGGGTATTTCTCACCCTGGAGAAAATAATGAAGTATATAGCAACATCTGTAGTGGCAATGCTGCTCTTATCGGG
TTGCGATAATACGCAAAGTAACAATTCATCCCCGTCAGAAACAGAAGTCGGCGTTGTTACACTCAAATCTCAACCGGTTT
CGGTAGTCAGTGAATTAACCGGACGCACCAGTGCTGCGCTCAGTGCTGAAGTACGTCCGCAGGTTGGGGGAATTATCCAG
AAACGCTTATTTAAGGAAGGTGATCTGGTCAAGGCTGGACAGCCTCTCTACCAGATTGATGCGGCCAGTTATCAGGCTGC
ATGGAATGAAGCCCGGGCAGCATTACAACAAGCACAGGCACTGGTAAAAGCCGATTGCCAGAAAGCGCAGCGTTATGCCC
GACTGGTGAAAGAGAATGGTGTTTCACAACAGGATGCTGATGATGCTCAGTCTACCTGTGCACAAGATAAAGCCAGTGTA
GAGGCGAAAAAAGCCGCACTGGAAACTGCGCGCATTAATCTTGACTGGACCACGGTAACCGCACCGATTTCAGGGCGCAT
TGGCATTTCGTCGGTAACCCCTGGCGCACTGGTGACCGCGTCGCAAGATACAGCGTTAACGACTATTCGTGGTCTGGATA
CAATGTATGTCGACCTCACTCGCTCCAGTGTCGATTTATTACGTCTGCGTAAACAGTCACTGGCGACCAACAGTGACACC
ATGAGCGTCTCACTTATTCTGGAAGATGGCACAACCTACAGCGAAAAAGGGCGTCTGGAACTCACCGAAGTCGCTGTAGA
TGAGTCTACCGGTTCGGTGACATTACGGGCGATTTTCCCCAATCCACAACAGCAGTTATTACCGGGAATGTTTGTTCGCG
CTCGTGTCGATGAAGGCGTGATGGAAGACGCTATTCTCGCGCCGCAACAGGGCGTCACGCGCGATGCTAAAGGCAATGCA
ACTGCGCTGGTGGTGAATAAAGACAATAAAGTAGAGCAGCGAGCGCTCGAAACGGGAGAAACGTATGGTGATAAATGGCT
GGTGCTGAACGGCCTGCACAACGGCGACCGACTGATTGTTGAAGGTTCTGCCAAAGTCACTTCAGGGCAGACCGTCAAGG
CTGTTGAAGTTCAGGCTAATGGAGGCAACTGA

Upstream 100 bases:

>100_bases
ATAGCGGGGGATATTAATCCCCCTTATTTCATTGCGAAATTCATTTCGCGATGCGGGTTCGTTATAACTGAATATCCCTG
ACATATCCTCATTAATTTCA

Downstream 100 bases:

>100_bases
ACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGA
AGTCCGTCAACGGGCAGTCC

Product: putative efflux protein, membrane fusion (MFP) family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQ
KRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASV
EAKKAALETARINLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNA
TALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGN

Sequences:

>Translated_383_residues
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQ
KRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASV
EAKKAALETARINLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNA
TALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGN
>Mature_383_residues
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQ
KRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASV
EAKKAALETARINLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNA
TALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=381, Percent_Identity=49.8687664041995, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=48.1382978723404, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=46.9101123595506, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082036, Length=325, Percent_Identity=28.3076923076923, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081796, Length=328, Percent_Identity=24.6951219512195, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40999; Mature: 40999

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHC
GRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQA
CCCHHHHEECCCCHHHHHHHHHHHCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
LVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETARINLDWTTVT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEE
APISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
ECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGV
EEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEECCCCCH
MEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIV
HHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEE
EGSAKVTSGQTVKAVEVQANGGN
ECCCEECCCCEEEEEEEEECCCC
>Mature Secondary Structure
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHC
GRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQA
CCCHHHHEECCCCHHHHHHHHHHHCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
LVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETARINLDWTTVT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEE
APISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
ECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGV
EEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEECCCCCH
MEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIV
HHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEE
EGSAKVTSGQTVKAVEVQANGGN
ECCCEECCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]