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The map label for this gene is rstA

Identifier: 218699826

GI number: 218699826

Start: 1525626

End: 1526354

Strand: Reverse

Name: rstA

Synonym: ECIAI39_1450

Alternate gene names: 218699826

Gene position: 1526354-1525626 (Counterclockwise)

Preceding gene: 218699828

Following gene: 218699825

Centisome position: 29.74

GC content: 48.97

Gene sequence:

>729_bases
GTGAATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTTCACTGATTGCTGCGTACCTGGCAAAACA
TGATATGCAGGTTACCGTGGAACCGCGCGGCGACCGGGCCGAAGAAACTATTCTGCGAGAAAATCCGGATTTGGTGTTAC
TCGATATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGATTGTTCTT
CTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGCGCCTGCGACTATATTCTCAAAACGACGCC
CCCTGCTGTTTTGCTAGCACGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCTACAGTGACCAAAGGTATTCAGGAAA
CGTCTCTGACTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCATCGATCCCATCAACCGCGTAGTCACCCTGGCTAAC
ACTGAAATCTCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGA
TGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGTCTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAA
AAAAACTGCTCGATAACGCCGCAGAACCTTATCGTATTAAAACCGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCG
TGGGAATAA

Upstream 100 bases:

>100_bases
CATAACAAGTCATCAGTAGAATACCTGATGAAACCTTGTTTAGAAACGATTGATAGTAAGTAAAAACAGCGCGGTGTATT
GTGACGTTTTTATATCTACC

Downstream 100 bases:

>100_bases
GCGATGAAAAAACTGTTTATCCAGTTTTACCTGTTATTGTTTGTCTGCTTCCTTGTGATGTCTCTGCTGGTCGGGCTGGT
GTACAAATTTACCGCCGAAC

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNVMNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVL
LTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLAN
TEISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHA
WE

Sequences:

>Translated_242_residues
MNVMNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVL
LTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLAN
TEISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHA
WE
>Mature_242_residues
MNVMNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVL
LTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLAN
TEISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHA
WE

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=98.744769874477, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=33.9055793991416, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87082012, Length=230, Percent_Identity=32.6086956521739, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=32.4675324675325, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=32.8947368421053, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=32.618025751073, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1786599, Length=234, Percent_Identity=29.9145299145299, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786911, Length=234, Percent_Identity=29.0598290598291, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=30.0429184549356, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=27.7056277056277, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=28.8793103448276, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787375, Length=241, Percent_Identity=28.2157676348548, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790552, Length=233, Percent_Identity=28.755364806867, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27063; Mature: 27063

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVMNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPG
CCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECC
KDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLR
CCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHC
QNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHA
CCCCHHHHCCCHHCCCCCCHHEEEEEEEECCCCEEEEEECCEEEEECHHHHHHHHHHHHH
GQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCC
WE
CC
>Mature Secondary Structure
MNVMNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPG
CCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECC
KDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLR
CCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHC
QNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHA
CCCCHHHHCCCHHCCCCCCHHEEEEEEEECCCCEEEEEECCEEEEECHHHHHHHHHHHHH
GQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCC
WE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]