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The map label for this gene is folM

Identifier: 218699828

GI number: 218699828

Start: 1526806

End: 1527528

Strand: Reverse

Name: folM

Synonym: ECIAI39_1452

Alternate gene names: 218699828

Gene position: 1527528-1526806 (Counterclockwise)

Preceding gene: 218699829

Following gene: 218699826

Centisome position: 29.76

GC content: 51.31

Gene sequence:

>723_bases
ATGGGTAAAACCCAGTCCTTGCCAATATTAATTACTGGCGGAGGTCGCCGCATCGGCCTCGCCCTCGCATGGCATTTCAT
TAATCAAAAGCAACCGGTGATTGTCAGCTATCGGACGCACTATCCCACCATTGATGGACTGATTAAGGCAGGTGCGCAGT
GTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGT
GCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGCA
GATCCACGTTAATACCCCATACCTGCTCAACCATGCTCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATA
TCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCCTATGCTGCAAGCAAAGCGGCACTGGAT
AATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAA
TGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGA
TCGACCTGGTCGATTACTTACTTACCAGCTGCTTTGTCACCGGACGCAGTCTCCCACTTGATGGCGGTCGTCATCTGCGT
TAA

Upstream 100 bases:

>100_bases
AATGGTGCTGATTGGCATGCTGTTAATTGCCTCAGTTCCGGCGACCTGGATGCTGGTGGGATAACGTTCATCCCATCGTT
GGATAGCAAAGGAGATAACG

Downstream 100 bases:

>100_bases
TGCAGCTTAATCCAGCATATAATCAACACCCATGCGCTTATTCCCCAGCACGCAACAGAGCCAACAAAAGCAGCGGGCAA
GCGCATCATTCCGGTGAAAT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTIDGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLR
AILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR

Sequences:

>Translated_240_residues
MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTIDGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLR
AILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR
>Mature_239_residues
GKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTIDGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRA
ILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDN
MTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR

Specific function: Catalyzes the reduction of dihydrofolate to tetrahydrofolate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787891, Length=240, Percent_Identity=97.9166666666667, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=25.4098360655738, Blast_Score=61, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28571526, Length=248, Percent_Identity=26.6129032258064, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 26379; Mature: 26248

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTIDGLIKAGAQCIQADFS
CCCCCCCCEEEECCCCEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTIDGLIKAGAQCIQADFS
CCCCCCCEEEECCCCEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]