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The map label for this gene is tnsB [H]

Identifier: 218697196

GI number: 218697196

Start: 3998093

End: 4000216

Strand: Reverse

Name: tnsB [H]

Synonym: EC55989_3928

Alternate gene names: 218697196

Gene position: 4000216-3998093 (Counterclockwise)

Preceding gene: 218697197

Following gene: 218697195

Centisome position: 77.6

GC content: 36.49

Gene sequence:

>2124_bases
ATGGATATTATTAGGAATTCAGTCTGGCTCTCTCAAGGCACTGATTTGCTTGCAGAGGGACTTTACCGTGTTTTGGATTT
TGACAGAAAGGTCGATTTGTTAATTTTGTTTAAAATAAAATCAGAAAGAACGGGTAAGCCAATCCCTTTCTCATTTTCAA
TGTTTAAATATTATATTGAATCAAATAGCATAACTTGTAAAGATTATATATATCCTTCATATATGTTAGTAGATGAGAAA
GAATTAACAGATAAAGACAGAGGAAGGCGAGATGAAAATTACAATATCATTAAAGATCTTGTAGATGACAGAATGTTTCT
GTTCGACTATGCATTACATAAAAAGTCGCACCTTTTAATGGATTACTCAAGAAATAAAAAAATATCACAATATACTATCA
GAACGCTTCTGGCGTTGTATTGGCGACATGGGCAGGATATTTATGCATTGCTACCCGCATTCTCGAACTGTGGCGCCGCT
GGGAAAAGTAGAATCAAACATGAAATTAAACTTGGGAATTCCAAAAAAAACAGAGCATTACCTAATGAACGATCACGTGT
TTTCATTCTTAATGAAAGAGATATAAATAATATTAGAAAATCTCTCATTACGTATCATTATAAAGTTAACGGAGATACGA
TAAAGAAAACATTAGAGAGACATATTGATTTGTATTTTAGGGATGAAATTAAAACAGCAAACCTAGAAAATAGAGCTCCA
TATGTTCCTTCTTTAAAACAATTTTCATACTGGAATAAAAAACTCTTCACTAAAGATTTCTCGATAAATAAGAAGAACAC
GAAAAAAGAAATAGACCTCAAAATGCGGGCGCTTTTAGGTAGTGTGGCTAATACAACTGTTTTGCCGGGTGATGTTTTCG
AAATAGACTCAACTGTCGCTGATGTACATCTTATTTCGAGTTTAAACAGACGAAAAGTCATTGGGCGTCCTACTATTTAT
ACAGTGGTGGATCGTGCAACAAGAATGATTGTTGGCCTTCATGTTTCTTTATACCATGCTTCATGGCGAGCCGCCCGACA
AGCGTTAGCAAATTGCTTTATGCCAAAAAAAGAATATTGTAGATTATTTGGAATCTCTATTACTGATGATGATTGGCCTT
GTTCTCACATTCCATTAACATTGATGTGTGACAACGGGGAGATGATTGGTCTTAAACCTCAGGAAGAGATGACCCCCCTG
ACAAAACTTGAGTTTGCGCCAGTCGGTAGAGGCGACAGAAAAAGCATTGTTGAACGCTGTTTCGGCATTCTGAATGATGA
GGTTATTCATAGGCTTATTGGAACAACACGAAGGGGAAAGATTGTTAAAGGAGAGCCAACACCGCAATCCAGGGCTTGTT
TAACGATTCAGGAGGTCACGTCTTTACTGATTCGGGAGATACTTGCACATAATCAGAGAACGTATGAGGAACTGGCTTAT
ATCAATCCCTTACTGATTGAAAATGATCTCGTAATATCACCAAAAAATAGTTGGATGATAAGCTTAAAGCACGGGAGATT
CAGCGCAAGAGCCGTTGGGGCCGACGAAGTGATCGCACGATTGTTAATCCCTGTGAACGCTAACATTACCGCCGGTGGGA
TTCAGTACAATAATCTTTTTTATGAATGTGATCCTGAAATTGCATCAGGTGTCAGAGTATTTGGAAGAACCACTTGTGAA
GCAAGAATAGATGACAATTGCGTAGATTATATATATGTAAGATTTGACAAAAATAGCATTTTCAAAAAACATTACCTACT
TAAGAAAAGAGATGTATTTAAAGGGAAAGCTCATCTTGATACAGATGTAATGGCTGATTGGGTAGATACTCAAAAAGAAA
TTAATTTATTTACTCTGGATTCGCTCAGCAATATCAATAATAAAGATGAATTTAACAGGAAAGGTAACGAAAGATTAAAT
GAAATATACGAATCTCGCCGGGTACATGGTAAAGATATTAAAAAGAACAGGAAAAATGAACTTGATTCATTAGGGCGAAC
TCATGTTGCTAATGGAAGATCTGAACCCCTACTTCCTTCATCCAGTAATGTTCTGCTTTTGCCTGGCCGGGAGGAGAAAC
AAAGATGGTTAAAAGGTAAGAAGAAGACTGAGGACGCAGAATAA

Upstream 100 bases:

>100_bases
ATTGGATTAAAAATGATAAATGTTGATTTATCATATCCAATACTCGAAACCGGTCTGATAAAGATAATTTCCAATGACCA
CTATATAGGACTGAACGCAA

Downstream 100 bases:

>100_bases
TGAATCTTATACGGCGCGTTTCAGCGATATATAAAGAACAGGAGTTACCTGAGTATCGTGGCAATCCCTTAATTGAAGCA
CTTCCTGAAGCGCTTACGGA

Product: Tn7-like transposition protein TnsB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERTGKPIPFSFSMFKYYIESNSITCKDYIYPSYMLVDEK
ELTDKDRGRRDENYNIIKDLVDDRMFLFDYALHKKSHLLMDYSRNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAA
GKSRIKHEIKLGNSKKNRALPNERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRAP
YVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDSTVADVHLISSLNRRKVIGRPTIY
TVVDRATRMIVGLHVSLYHASWRAARQALANCFMPKKEYCRLFGISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPL
TKLEFAPVGRGDRKSIVERCFGILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELAY
INPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYNNLFYECDPEIASGVRVFGRTTCE
ARIDDNCVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEINLFTLDSLSNINNKDEFNRKGNERLN
EIYESRRVHGKDIKKNRKNELDSLGRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE

Sequences:

>Translated_707_residues
MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERTGKPIPFSFSMFKYYIESNSITCKDYIYPSYMLVDEK
ELTDKDRGRRDENYNIIKDLVDDRMFLFDYALHKKSHLLMDYSRNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAA
GKSRIKHEIKLGNSKKNRALPNERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRAP
YVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDSTVADVHLISSLNRRKVIGRPTIY
TVVDRATRMIVGLHVSLYHASWRAARQALANCFMPKKEYCRLFGISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPL
TKLEFAPVGRGDRKSIVERCFGILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELAY
INPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYNNLFYECDPEIASGVRVFGRTTCE
ARIDDNCVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEINLFTLDSLSNINNKDEFNRKGNERLN
EIYESRRVHGKDIKKNRKNELDSLGRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE
>Mature_707_residues
MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERTGKPIPFSFSMFKYYIESNSITCKDYIYPSYMLVDEK
ELTDKDRGRRDENYNIIKDLVDDRMFLFDYALHKKSHLLMDYSRNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAA
GKSRIKHEIKLGNSKKNRALPNERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRAP
YVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDSTVADVHLISSLNRRKVIGRPTIY
TVVDRATRMIVGLHVSLYHASWRAARQALANCFMPKKEYCRLFGISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPL
TKLEFAPVGRGDRKSIVERCFGILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELAY
INPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYNNLFYECDPEIASGVRVFGRTTCE
ARIDDNCVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEINLFTLDSLSNINNKDEFNRKGNERLN
EIYESRRVHGKDIKKNRKNELDSLGRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 81770; Mature: 81770

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERTGKPIPFSFSMFKYYIE
CCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHEEEEE
SNSITCKDYIYPSYMLVDEKELTDKDRGRRDENYNIIKDLVDDRMFLFDYALHKKSHLLM
CCCEEEEHCCCCCEEEEECHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCEEE
DYSRNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAAGKSRIKHEIKLGNSKKNRAL
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCHHHHHHHHEECCCCCCCCCC
PNERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRAP
CCCCCEEEEEECCCHHHHHHHHEEEEEEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCC
YVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDSTVA
CCCCHHHHHHHCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEECCHHHH
DVHLISSLNRRKVIGRPTIYTVVDRATRMIVGLHVSLYHASWRAARQALANCFMPKKEYC
HHHHHHCCCCCEECCCCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHCCCHHHHH
RLFGISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPLTKLEFAPVGRGDRKSIVERC
EEEECEECCCCCCCCCCCEEEEECCCCEECCCCHHHCCCCHHEEECCCCCCHHHHHHHHH
FGILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELAY
HHHHCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHH
INPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYNNLF
CCCEEEECCEEECCCCCEEEEEECCCCEEECCCHHHHHHHHHEECCCCEEECCEEECCEE
YECDPEIASGVRVFGRTTCEARIDDNCVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLD
EEECHHHHCCCEECCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
TDVMADWVDTQKEINLFTLDSLSNINNKDEFNRKGNERLNEIYESRRVHGKDIKKNRKNE
HHHHHHHHCCHHHCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LDSLGRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE
HHHHCCHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERTGKPIPFSFSMFKYYIE
CCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHEEEEE
SNSITCKDYIYPSYMLVDEKELTDKDRGRRDENYNIIKDLVDDRMFLFDYALHKKSHLLM
CCCEEEEHCCCCCEEEEECHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCEEE
DYSRNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAAGKSRIKHEIKLGNSKKNRAL
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCHHHHHHHHEECCCCCCCCCC
PNERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRAP
CCCCCEEEEEECCCHHHHHHHHEEEEEEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCC
YVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDSTVA
CCCCHHHHHHHCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCEECCHHHH
DVHLISSLNRRKVIGRPTIYTVVDRATRMIVGLHVSLYHASWRAARQALANCFMPKKEYC
HHHHHHCCCCCEECCCCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHCCCHHHHH
RLFGISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPLTKLEFAPVGRGDRKSIVERC
EEEECEECCCCCCCCCCCEEEEECCCCEECCCCHHHCCCCHHEEECCCCCCHHHHHHHHH
FGILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHNQRTYEELAY
HHHHCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHH
INPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANITAGGIQYNNLF
CCCEEEECCEEECCCCCEEEEEECCCCEEECCCHHHHHHHHHEECCCCEEECCEEECCEE
YECDPEIASGVRVFGRTTCEARIDDNCVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLD
EEECHHHHCCCEECCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
TDVMADWVDTQKEINLFTLDSLSNINNKDEFNRKGNERLNEIYESRRVHGKDIKKNRKNE
HHHHHHHHCCHHHCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LDSLGRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKTEDAE
HHHHCCHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]