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The map label for this gene is tnsA [H]

Identifier: 218697197

GI number: 218697197

Start: 4000203

End: 4001045

Strand: Reverse

Name: tnsA [H]

Synonym: EC55989_3929

Alternate gene names: 218697197

Gene position: 4001045-4000203 (Counterclockwise)

Preceding gene: 218697198

Following gene: 218697196

Centisome position: 77.62

GC content: 40.09

Gene sequence:

>843_bases
ATGGCTAGGGGTAGGCGTCTCAAATCCTATTTGGATTATGAAAATGCGCTAGGTGACGGCATAGGAGTGGGCTATGGCCA
AAGTTATCAGCCCTGGCTTAGAGCTCAGGACGTTAAATCCCGTGGAAACCGTTCGATAGTCTTTGGCCTTAAGACGTTTC
GAAACCATCATCTCCTTTCTTCTGTCGAAAGTAACTTTTTCTATCTGGCTGAGTTTAATGACTCGGTGATTGATATCCGG
GAACAATTCCCACTCTTTCCTCTCCGGCTTACCCAACAAATAGCAAATCATCTACATTTTCAACATCCTATGGTGAGGGG
AGTAAGAGGAGTACCTGTCGAAGTTCTGAATGTTATGACAACCGATTTTTTACTGACCTTGAGAACTCCTGAAGGCGGAC
TTCGATACAAAGCTATAGCAGTAAAACATAACGAGAGCATACCTGAACGCGAAGCCCAAAAACTTGAAATAGAGAGGATG
TTTTGGCAGTTGATTGATGTTGAGTTTCAAATTTATGTTGGCTCGGAACTCAATAACGTCGTCGGTAAAAACATTTGCTG
GGCTACTTCTGTATTAAGAGATGGTTCTGAATTTTATGATAAATATCCTCTTGATAAAATCCTATGGAAGCTTAAACCAG
ATGTTTATCCCATAGTAGGACTACGTGCAATGATTTCATCAATCTTTGGGGTAGATGCACAAGAAGCGATGATGTTATTG
CAGGCAATGATTGGATTAAAAATGATAAATGTTGATTTATCATATCCAATACTCGAAACCGGTCTGATAAAGATAATTTC
CAATGACCACTATATAGGACTGAACGCAAATGGATATTATTAG

Upstream 100 bases:

>100_bases
CGACGCTAATGTTGTATACTTACTGAATGTGTGGTTATAGGGTTGGAGACTATAAAATGCACATATAGGTACGTATCAAT
GTCGTTTTTAGGGTACGATC

Downstream 100 bases:

>100_bases
GAATTCAGTCTGGCTCTCTCAAGGCACTGATTTGCTTGCAGAGGGACTTTACCGTGTTTTGGATTTTGACAGAAAGGTCG
ATTTGTTAATTTTGTTTAAA

Product: Tn7-like transposase TnsA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNRSIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIR
EQFPLFPLRLTQQIANHLHFQHPMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERM
FWQLIDVEFQIYVGSELNNVVGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPIVGLRAMISSIFGVDAQEAMMLL
QAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNANGYY

Sequences:

>Translated_280_residues
MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNRSIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIR
EQFPLFPLRLTQQIANHLHFQHPMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERM
FWQLIDVEFQIYVGSELNNVVGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPIVGLRAMISSIFGVDAQEAMMLL
QAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNANGYY
>Mature_279_residues
ARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNRSIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIRE
QFPLFPLRLTQQIANHLHFQHPMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERMF
WQLIDVEFQIYVGSELNNVVGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPIVGLRAMISSIFGVDAQEAMMLLQ
AMIGLKMINVDLSYPILETGLIKIISNDHYIGLNANGYY

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 32109; Mature: 31977

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNRSIVFGLKTFRNHHLLS
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHCCHHHHH
SVESNFFYLAEFNDSVIDIREQFPLFPLRLTQQIANHLHFQHPMVRGVRGVPVEVLNVMT
HHCCCEEEEEECCCCEEHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
TDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERMFWQLIDVEFQIYVGSELNNV
HEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCHHHH
VGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPIVGLRAMISSIFGVDAQEAMMLL
HCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
QAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNANGYY
HHHHCEEEEEEECCCCHHHHCEEEEECCCEEEEECCCCCC
>Mature Secondary Structure 
ARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNRSIVFGLKTFRNHHLLS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHCCHHHHH
SVESNFFYLAEFNDSVIDIREQFPLFPLRLTQQIANHLHFQHPMVRGVRGVPVEVLNVMT
HHCCCEEEEEECCCCEEHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
TDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERMFWQLIDVEFQIYVGSELNNV
HEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCHHHH
VGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPIVGLRAMISSIFGVDAQEAMMLL
HCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
QAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNANGYY
HHHHCEEEEEEECCCCHHHHCEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]