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The map label for this gene is agaR

Identifier: 218696836

GI number: 218696836

Start: 3632823

End: 3633632

Strand: Reverse

Name: agaR

Synonym: EC55989_3549

Alternate gene names: 218696836

Gene position: 3633632-3632823 (Counterclockwise)

Preceding gene: 218696853

Following gene: 218696832

Centisome position: 70.49

GC content: 52.22

Gene sequence:

>810_bases
ATGAGTAATACCGACGCTTCAGGTGAGAAGCGAGTGACTGGCACCAGCGAGCGACGAGAACAGATCATTCAGCGTCTGCG
ACAGCAAGGGAGTGTGCAGGTTAACGATCTGTCGGCATTGTATGGCGTATCTACTGTGACGATCCGCAACGATCTGGCGT
TTCTGGAAAAGCAGGGGATCGCTGTGCGTGCCTATGGTGGCGCGTTGATCTGCGATAGCACGACGCCGTCAGTCGAGCCA
TCAGTGGAAGATAAAAGCGCACTGAACACCGCGATGAAACGCAGCGTTGCGAAAGCTGCCGTTGAGTTGATTCAGCCAGG
TCATCGGGTGATCCTCGATTCCGGGACCACCACTTTTGAGATTGCTCGTCTGATGCGCAAGCACACTGACGTAATTGCGA
TGACCAACGGTATGAACGTGGCTAATGCGTTGCTGGAAGCCGAAGGCGTTGAGCTGCTGATGACCGGCGGGCATTTGCGC
CGTCAGTCGCAATCTTTTTACGGCGATCAGGCTGAGCAATCGCTGCAAAATTACCACTTCGATATGCTGTTTCTTGGTGT
AGATGCGATCGATCTGGAGCGCGGCGTCAGCACACATAATGAAGATGAAGCCCGTTTAAACCGTCGGATGTGCGAAGTTG
CGGAACGGATCATCGTGGTCACCGATTCCAGTAAATTCAATCGTTCCAGTTTACATAAGATCATTGATACTCAACGTATC
GACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGTCTGGAAGGACTGCGAAAGGCTGGGGTTGAAGTGATTCTGGT
CGGGGAGTGA

Upstream 100 bases:

>100_bases
GTAGCTTGCGTCAATGGGCAAGGTGGGCTTGCATTTGCTTAATAGAAAGGCGTTAATAGGCAAAACGAAATGAAACGAAA
GTTTTACGAAAGGACTTACT

Downstream 100 bases:

>100_bases
GAAAGGGTGCCCGCTGGTCAACACGAATACGTTCGGCGGGCATTGTGAACACCATCAATGGTTTTCTTCTGTCTCTTGGG
TGAGGCTTTCCCAGTCGGCA

Product: DNA-binding transcriptional regulator AgaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVSTVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEP
SVEDKSALNTAMKRSVAKAAVELIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTDSSKFNRSSLHKIIDTQRI
DMIIVDEGIPADSLEGLRKAGVEVILVGE

Sequences:

>Translated_269_residues
MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVSTVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEP
SVEDKSALNTAMKRSVAKAAVELIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTDSSKFNRSSLHKIIDTQRI
DMIIVDEGIPADSLEGLRKAGVEVILVGE
>Mature_268_residues
SNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVSTVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPS
VEDKSALNTAMKRSVAKAAVELIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLRR
QSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTDSSKFNRSSLHKIIDTQRID
MIIVDEGIPADSLEGLRKAGVEVILVGE

Specific function: Probable repressor for the aga operon for N-acetyl galactosamine transport and metabolism

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789519, Length=269, Percent_Identity=100, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1789059, Length=254, Percent_Identity=35.4330708661417, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI226510968, Length=246, Percent_Identity=30.8943089430894, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789829, Length=251, Percent_Identity=28.2868525896414, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787540, Length=251, Percent_Identity=31.8725099601594, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082344, Length=254, Percent_Identity=27.1653543307087, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788069, Length=250, Percent_Identity=24.8, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787063, Length=240, Percent_Identity=27.5, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790753, Length=250, Percent_Identity=26, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790635, Length=254, Percent_Identity=27.1653543307087, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789170, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AGAR_ECO57 (P0ACK4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85975
- PIR:   A91130
- RefSeq:   NP_289702.1
- RefSeq:   NP_312036.1
- ProteinModelPortal:   P0ACK4
- SMR:   P0ACK4
- EnsemblBacteria:   EBESCT00000027986
- EnsemblBacteria:   EBESCT00000056407
- GeneID:   916155
- GeneID:   958202
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4483
- KEGG:   ecs:ECs4009
- GeneTree:   EBGT00070000031704
- HOGENOM:   HBG694151
- OMA:   TTTYEIA
- ProtClustDB:   PRK09802
- BioCyc:   ECOL83334:ECS4009-MONOMER
- GO:   GO:0005622
- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00420

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR

EC number: NA

Molecular weight: Translated: 29573; Mature: 29442

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00894 HTH_DEOR_1; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVSTVTIRNDLAFLEKQGI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHCCEEEEEEHHHHHHHHCCE
AVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVELIQPGHRVILDSGTTTFE
EEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
IARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLRRQSQSFYGDQAEQSLQNYHF
HHHHHHHCCCEEEEECCCHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHCCC
DMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTDSSKFNRSSLHKIIDTQRI
EEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCC
DMIIVDEGIPADSLEGLRKAGVEVILVGE
CEEEEECCCCHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVSTVTIRNDLAFLEKQGI
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHCCEEEEEEHHHHHHHHCCE
AVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVELIQPGHRVILDSGTTTFE
EEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
IARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLRRQSQSFYGDQAEQSLQNYHF
HHHHHHHCCCEEEEECCCHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHCCC
DMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTDSSKFNRSSLHKIIDTQRI
EEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCC
DMIIVDEGIPADSLEGLRKAGVEVILVGE
CEEEEECCCCHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796