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The map label for this gene is b0257 [C]

Identifier: 218696584

GI number: 218696584

Start: 3368231

End: 3368803

Strand: Reverse

Name: b0257 [C]

Synonym: EC55989_3282

Alternate gene names: 218696584

Gene position: 3368803-3368231 (Counterclockwise)

Preceding gene: 218696585

Following gene: 218696583

Centisome position: 65.35

GC content: 54.97

Gene sequence:

>573_bases
GTGAAAATCGTGAACTGCGCCGCAGTAGCGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAGTTCGACCGC
CTCTGGAAAAAATAATGCCCTGCTGGATAAGCTGCGTGAGCAGTACGGGGTCGGACCGGTATGCAGTGAACTGCATATCG
CCCCGTCAACGAATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTTCCCGTGCGCAGCACGATGACTGG
CTGAAGAGAGAGATACAGCGCGTATACGATGAAAATCATCAGGTGTACGGTGTGCGTAAAGTCTGGCGCCAGTTGTTACG
GGAAGGAATCAGGGTGGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAA
AGGTCCGTACGACCATCAGCCGGAAAGCCTTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGAC
CAGCTGTGGGTGGCTGATTTTACTTACGGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTG
TCTGGAATATTGA

Upstream 100 bases:

>100_bases
GAGACCCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCAGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCA
GCGTCTGAAAGAGCTGGAGC

Downstream 100 bases:

>100_bases
ACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCAT
GCCCGCAGGGTGATGTAACC

Product: transposase ORF B (fragment), IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKIVNCAAVAISFARLPLILRRRSSTASGKNNALLDKLREQYGVGPVCSELHIAPSTNYHCQQQRHHPDKRSSRAQHDDW
LKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAFAAGDRVNRQFVAERPD
QLWVADFTYGVPGVQGGHGCLNEPRVCLEY

Sequences:

>Translated_190_residues
MKIVNCAAVAISFARLPLILRRRSSTASGKNNALLDKLREQYGVGPVCSELHIAPSTNYHCQQQRHHPDKRSSRAQHDDW
LKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAFAAGDRVNRQFVAERPD
QLWVADFTYGVPGVQGGHGCLNEPRVCLEY
>Mature_190_residues
MKIVNCAAVAISFARLPLILRRRSSTASGKNNALLDKLREQYGVGPVCSELHIAPSTNYHCQQQRHHPDKRSSRAQHDDW
LKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAFAAGDRVNRQFVAERPD
QLWVADFTYGVPGVQGGHGCLNEPRVCLEY

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=132, Percent_Identity=47.7272727272727, Blast_Score=122, Evalue=2e-28,
Organism=Escherichia coli, GI1789981, Length=141, Percent_Identity=30.4964539007092, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 21588; Mature: 21588

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVNCAAVAISFARLPLILRRRSSTASGKNNALLDKLREQYGVGPVCSELHIAPSTNYH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCC
CQQQRHHPDKRSSRAQHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARL
HHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
MAVMGLAGVLRGKKVRTTISRKAFAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGC
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC
LNEPRVCLEY
CCCCCEECCC
>Mature Secondary Structure
MKIVNCAAVAISFARLPLILRRRSSTASGKNNALLDKLREQYGVGPVCSELHIAPSTNYH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCC
CQQQRHHPDKRSSRAQHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARL
HHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
MAVMGLAGVLRGKKVRTTISRKAFAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGC
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC
LNEPRVCLEY
CCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]