Click here to switch to the map view.

The map label for this gene is hcaE

Identifier: 218696165

GI number: 218696165

Start: 2892385

End: 2893746

Strand: Direct

Name: hcaE

Synonym: EC55989_2824

Alternate gene names: 218696165

Gene position: 2892385-2893746 (Clockwise)

Preceding gene: 218696162

Following gene: 218696166

Centisome position: 56.11

GC content: 53.08

Gene sequence:

>1362_bases
ATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGA
CCCGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAA
AACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCG
TTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTA
TCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTA
AATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTGATTTTTGGCAACTGGGATACCAGCGCA
CCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAAT
TGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGACCAGTATCATG
CTCTGTTCAGCCATGCTTCTGCCGTTCAGGTATTAGGGGCGAAAGATGATGGCAGCGATAAGCGCCTCGGTGATGGACAA
ACCGCCCGCCCGGTGTGGGAAACCGCCAAAGATGCGCTGCAATTTGGTCAGGACGGTCACGGTAGCGGTTTCTTCTTTAC
TGAAAAACCGGATGCTAATGTCTGGGTCGATGGCGCAGTTTCAAGCTATTACCGCGAAACCTATGCCGAAGCAGAACAAC
GTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATATTCCCCACGCTTTCATGGCTAAACGGCACTGCC
ACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGA
TGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGA
ACTGGTGTGAAATCCAGAAATTGCTGAAAGGTCACCGCGCCCGCAACAGCAAACTGTGTCTGGAAATGGGGCTTGGTCAG
GAAAAGCGTCGCGACGACGGCATTCCAGGCATTACTAACTATATCTTCTCAGAAACGGCCGCTCGTGGAATGTACCAACG
CTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGTGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAAT
GA

Upstream 100 bases:

>100_bases
GGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTT
ATCTTTCAGGATTAAAAAAT

Downstream 100 bases:

>100_bases
GTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGTTTCTCTTTCACGAAGCCAGCTTACTGGACGACTGGAAA
TTTCGTGACTGGCTGGCGCA

Product: 3-phenylpropionate dioxygenase, large (alpha) subunit

Products: NA

Alternate protein names: Digoxigenin subunit alpha

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKA
FLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTA
TLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQ
EKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK

Sequences:

>Translated_453_residues
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKA
FLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTA
TLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQ
EKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK
>Mature_452_residues
TTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAF
LNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAP
GLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQT
ARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTAT
LRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQE
KRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain

Homologues:

Organism=Escherichia coli, GI1788888, Length=453, Percent_Identity=100, Blast_Score=948, Evalue=0.0,
Organism=Escherichia coli, GI1788103, Length=154, Percent_Identity=27.9220779220779, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HCAE_ECO57 (P0ABR6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91054
- PIR:   G85898
- RefSeq:   NP_289094.1
- RefSeq:   NP_311431.1
- ProteinModelPortal:   P0ABR6
- SMR:   P0ABR6
- EnsemblBacteria:   EBESCT00000026188
- EnsemblBacteria:   EBESCT00000056667
- GeneID:   913881
- GeneID:   957853
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3809
- KEGG:   ecs:ECs3404
- GeneTree:   EBGT00050000010714
- HOGENOM:   HBG705920
- OMA:   SENWVEI
- ProtClustDB:   CLSK880425
- BioCyc:   ECOL83334:ECS3404-MONOMER
- HAMAP:   MF_01648
- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663
- Gene3D:   G3DSA:2.102.10.10
- PANTHER:   PTHR21266:SF2
- PRINTS:   PR00090

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A; SSF50022 Rieske_dom

EC number: =1.14.12.19

Molecular weight: Translated: 51110; Mature: 50978

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS51296 RIESKE; PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFN
CCCCCCCEEEEEEECCCCEECCEEECCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHH
TYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELID
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCCCCCCCCCEEE
VPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAPGLRDYLGDIAWYLDGMLDR
CCCCCCCCHHHHHHHCCCCCCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHC
REGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
CCCCHHHHCCCEEEEEECCCCCCHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRA
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHE
LRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRA
EEECCCCCCCCEEEECCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCC
FGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQEKRRDDGIPGITNYIFSETA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHH
ARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWLFLAHESQIPKPGDFFN
CCCCCCEEEEEEECCCCEECCEEECCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHH
TYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRAFTCPYHGWSYGINGELID
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCEEECCCCCCCCCCCCCEEE
VPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSAPGLRDYLGDIAWYLDGMLDR
CCCCCCCCHHHHHHHCCCCCCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHC
REGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSHASAVQVLGAKDDGSDKRLGDGQ
CCCCHHHHCCCEEEEEECCCCCCHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
TARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWVDGAVSSYYRETYAEAEQRLGEVRA
CCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHE
LRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVEVWAFCITDKAASDEVKAAFENSATRA
EEECCCCCCCCEEEECCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCC
FGPAGFLEQDDSENWCEIQKLLKGHRARNSKLCLEMGLGQEKRRDDGIPGITNYIFSETA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHH
ARGMYQRWADLLSSESWQEVLDKTAAYQQEVMK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796