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The map label for this gene is yeiT

Identifier: 218695757

GI number: 218695757

Start: 2449659

End: 2450897

Strand: Direct

Name: yeiT

Synonym: EC55989_2396

Alternate gene names: 218695757

Gene position: 2449659-2450897 (Clockwise)

Preceding gene: 218695756

Following gene: 218695758

Centisome position: 47.52

GC content: 51.33

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGCCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTT
ATTATGTCATGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTC
GTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAG
AAATTATGTCAAAGCGGTTGTACCCGTGCCGGTGTTGATACTCCCATTGATATCGGTCGCTTACAGCGTTTTGTTACTGA
TTTTGAACAACAAACTGGGATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTC
CTGCCGGATTGCAGGCCAGTGTAACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGT
GGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAACAACGAAGTCGGTAACACACTCACCCTTGAGCAGCTGAAAGCAGAAAACCGCGCGGTAC
TGGTCACCGTGGGGTTATCGAGCGGTTCCGGGTTATCGCTGTTTGAGCATAGCGACGTTGAGATTGCCGTGGACTTCCTG
CAACGTGCACGACAGGCGCAAGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTTGCGATGGA
CGTAGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCGGTAACCTGCGTAGCGCGTGAAGAGTTAGATGAGTTTCCGGCAA
GCGAAAAAGAGTTTGCCAGCGCCCGGGAGCTGGGCGTTTCGATCATCGATGGATTCACGCCAGTAGCCGTGGAAGGCAAT
AAAGTCACGTTTAAGCATGTACGGTTACCGGGCGAACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCA
TGCCAGACTGGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTATCAGACCCGCGATCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
TGACTGGGGTGAGCGAACGCAGACGCAGCACATGCAACTTGAAGTATGACGAGTATATTTAGCGTTCGCCTTAATTACTC
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCCCTGTAGGCAACTGCT
ATGAGATGTGTGCCAAAGCC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGG
WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFLQ
RARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNK
VTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=32.0366132723112, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=48.8888888888889, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=98.7864077669903, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=449, Percent_Identity=31.8485523385301, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=31.6742081447964, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=29.5555555555556, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789243, Length=400, Percent_Identity=29.75, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=32.8798185941043, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=27.3942093541203, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=24.9457700650759, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=31.1059907834101, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=31.1059907834101, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=27.9279279279279, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=27.9279279279279, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=28.2511210762332, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=28.2511210762332, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44340; Mature: 44209

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEECCCCHHHHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLSLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELTMAADKIILAVGQHARLDAFAELEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEECCCCHHHHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]