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The map label for this gene is kefF

Identifier: 218693518

GI number: 218693518

Start: 49173

End: 49703

Strand: Direct

Name: kefF

Synonym: EC55989_0046

Alternate gene names: 218693518

Gene position: 49173-49703 (Clockwise)

Preceding gene: 218693517

Following gene: 218693519

Centisome position: 0.95

GC content: 54.99

Gene sequence:

>531_bases
ATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCT
GGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGT
CTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCACCGCTCCTCAAACTTTGGATCGATAAA
GTTTTCTCGCACGGCTGGGCTTACGGTCACGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGG
CGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCT
ACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCG
CGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAG

Upstream 100 bases:

>100_bases
AGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGG
CGTAATCACGCGGGCTACCT

Downstream 100 bases:

>100_bases
CCATACGCTGGTTCAGGCGCTGATCTACCTCGGATCGGCGGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAT
CGGTACTTGGCTACCTGATT

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDK
VFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAHHG

Sequences:

>Translated_176_residues
MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDK
VFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAHHG
>Mature_176_residues
MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDK
VFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAHHG

Specific function: Required for full activity of kefC

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily

Homologues:

Organism=Homo sapiens, GI156564357, Length=151, Percent_Identity=33.112582781457, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI4505415, Length=104, Percent_Identity=35.5769230769231, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1786231, Length=176, Percent_Identity=100, Blast_Score=365, Evalue=1e-103,
Organism=Escherichia coli, GI1789750, Length=165, Percent_Identity=40.6060606060606, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KEFF_ECO24 (A7ZHD9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461218.1
- ProteinModelPortal:   A7ZHD9
- SMR:   A7ZHD9
- STRING:   A7ZHD9
- EnsemblBacteria:   EBESCT00000021069
- GeneID:   5589117
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0050
- eggNOG:   COG2249
- GeneTree:   EBGT00050000010021
- HOGENOM:   HBG532247
- OMA:   QAQARHY
- ProtClustDB:   PRK00871
- BioCyc:   ECOL331111:ECE24377A_0050-MONOMER
- HAMAP:   MF_01414
- InterPro:   IPR003680

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 1.6.99.- [C]

Molecular weight: Translated: 20170; Mature: 20170

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIV
CEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECHHHHHHHHCEEE
WQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPG
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEEECCCCCCEEEECCCCC
FDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAHHG
HHHHHCCHHHHEEEECCCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIV
CEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECHHHHHHHHCEEE
WQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPG
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEEECCCCCCEEEECCCCC
FDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAHHG
HHHHHCCHHHHEEEECCCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA