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The map label for this gene is nanA2 [H]

Identifier: 218692463

GI number: 218692463

Start: 4868995

End: 4869912

Strand: Direct

Name: nanA2 [H]

Synonym: ECED1_4911

Alternate gene names: 218692463

Gene position: 4868995-4869912 (Clockwise)

Preceding gene: 218692462

Following gene: 218692464

Centisome position: 93.46

GC content: 38.24

Gene sequence:

>918_bases
ATGCAATGTGAATTTAAAGGGGTCATATCAGCATTACCCACTCCTTATGACCAATCCCAGCAAATCGATATGGAGAGTCT
TCGAAAACTGATCCGTTTTAATATCGAACAAAATATTAAGGGATTGTATGTTGGAGGCTCTACTGGAGAAGCATTTCTTC
AGAATGTTGCAGAGCGAGAAAAAATTCTTGAAACAGTAGCCGATGAATCAGATGGAAGACTGACACTTATAGCGCATGTG
GGCGGAATCAGTACGGCAGAAAGCGAGGTACTTGCAAAAGCTGCAAAAAAATACGGATATCATGCTATATCTGCTGTTAC
TCCATTTTATTATCCTTTTTCATTTGAAGAGCATTGTATCCATTACAGGAAGATTATTGATTCTGCAGATGGACTTCCGA
TGGTAGTTTATAATATACCGGCTCTTAGCGGAGTGCGTTTTTCTTTAGATCAGATAAATGAGCTTGTCACAATACCCAGG
GTTTGTGCATTAAAACAGACATCCGGTGATTTATTCCAGATGGAACAAATCAAAAGGAATCATCCAGAGCTTGTGTTATA
TAATGGTTACGATGAAATATTTGCATCAGGGTTAATTGCAGGTGCAGATGGGGGAATCGGTAGTACATATAATATAATGG
GGTGGCGTTATCTTGAAATATTTGAAGCAGTCAAAAACAATGATGTGATTAAGGCGAAGGAAATACAGGTAGCCTGTAAC
CAGGTTATTGACACTCTTATTCAGAGTGGTGTCCTGGCAGGTATAAAAACTCTTCTTTACTATATGGGGATTATAAATAC
TCCTGTATGCAGAAGTCCTTTTTCTCCGGTAAAGGAAAAGAATCTGGATGTATTATCAAAACTTGCAGAGCGACTGCTCG
AAGAACATGACAGAAACAAAAAAATGAAAATAATCTGA

Upstream 100 bases:

>100_bases
TTTGATAATCTTCACATTGTGCTTCTTTTCTGTTGGATAATCTTTGTACTTGGTGGTGTACTAGGTATCCCATTATGCAG
TTAAGTGAAGGTGATTAAAA

Downstream 100 bases:

>100_bases
ATAATTGCAAGATTGTTATATAGGTGAAAATGAATGATAACACTGGCTGTTGATATAGGAGGAACGAAAATTTCAGCGGC
GTTGATATCTGATGACGGTT

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase 2; N-acetylneuraminic acid aldolase 2; Sialate lyase 2; Sialic acid aldolase 2; Sialic acid lyase 2 [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYVGGSTGEAFLQNVAEREKILETVADESDGRLTLIAHV
GGISTAESEVLAKAAKKYGYHAISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYLEIFEAVKNNDVIKAKEIQVACN
QVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSPVKEKNLDVLSKLAERLLEEHDRNKKMKII

Sequences:

>Translated_305_residues
MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYVGGSTGEAFLQNVAEREKILETVADESDGRLTLIAHV
GGISTAESEVLAKAAKKYGYHAISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYLEIFEAVKNNDVIKAKEIQVACN
QVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSPVKEKNLDVLSKLAERLLEEHDRNKKMKII
>Mature_305_residues
MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYVGGSTGEAFLQNVAEREKILETVADESDGRLTLIAHV
GGISTAESEVLAKAAKKYGYHAISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYLEIFEAVKNNDVIKAKEIQVACN
QVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSPVKEKNLDVLSKLAERLLEEHDRNKKMKII

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=254, Percent_Identity=28.3464566929134, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31543060, Length=290, Percent_Identity=23.448275862069, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1789620, Length=296, Percent_Identity=66.2162162162162, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1788823, Length=276, Percent_Identity=25.3623188405797, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082415, Length=238, Percent_Identity=26.4705882352941, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786463, Length=233, Percent_Identity=26.6094420600858, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 34047; Mature: 34047

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00665 DHDPS_1 ; PS00666 DHDPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYVGGSTGEAFLQNVAERE
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCHHCCCCEEEEECCCHHHHHHHHHHHH
KILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHAISAVTPFYYPFSFEEHCI
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
HYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPRVCALKQTSGDLFQMEQIKRN
HHHHHHHCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
HPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYLEIFEAVKNNDVIKAKEIQVACN
CCCEEEECCHHHHHHCCCEECCCCCCCCCCHHCCHHHHHHHHHHCCCCCEEHHHHHHHHH
QVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSPVKEKNLDVLSKLAERLLEEHDRNK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCC
KMKII
CCCCC
>Mature Secondary Structure
MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYVGGSTGEAFLQNVAERE
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCHHCCCCEEEEECCCHHHHHHHHHHHH
KILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHAISAVTPFYYPFSFEEHCI
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
HYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPRVCALKQTSGDLFQMEQIKRN
HHHHHHHCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
HPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYLEIFEAVKNNDVIKAKEIQVACN
CCCEEEECCHHHHHHCCCEECCCCCCCCCCHHCCHHHHHHHHHHCCCCCEEHHHHHHHHH
QVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSPVKEKNLDVLSKLAERLLEEHDRNK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCC
KMKII
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]