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The map label for this gene is thiE [H]

Identifier: 218692274

GI number: 218692274

Start: 4652879

End: 4653514

Strand: Reverse

Name: thiE [H]

Synonym: ECED1_4700

Alternate gene names: 218692274

Gene position: 4653514-4652879 (Counterclockwise)

Preceding gene: 218692275

Following gene: 218692273

Centisome position: 89.33

GC content: 58.18

Gene sequence:

>636_bases
ATGTATCAGCCTGAGTTTCCTCCTGTACCCTTTCGTTTAGGACTGTACCCGGTGGTTGACAGCGTACAGTGGATCGAACG
TCTGTTGGATGCAGGCGTACGTACTCTCCAGCTACGCATCAAAGATCGGCGCGATGAAGAGGTAGAAGCCGATGTGGTGG
CGGCAATTGCGCTGGGCCGCCGCTATAACGCGCGATTGTTTATCAACGATTACTGGCGGCTGGCGATTAAGCATCAGGCG
TATGGCGTCCATCTGGGGCAGGAAGATTTGCAAGCCACCGATCTCAGCGCCATCCGCGCGGCAGGCCTGCGGCTGGGCGT
TTCGACCCATGACGATATGGAGATCGACGTCGCGCTGGCGGAACGTCCTTCTTATATCGCGCTGGGACATGTGTTTCCGA
CACAAACCAAACAGATGCCTTCTGCACCGCAGGGACTGGAACAGCTGGCACGGCATGTTGAGCGCCTGGCGGATTATCCC
ACCGTGGCGATTGGCGGTATCAGTCTGGCGCGCGCGCCTGCGGTGATAGCAACGGGTGTCGGCAGTATCGCCGTCGTCAG
CGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGATGAATGA

Upstream 100 bases:

>100_bases
ATTACGCCGCCGCGCAAACCATTGAAGTGGGAATGGCGGATATGTCGGAGAACTTCCGCGCCAGAGGCGGAGAAATCTAC
CTGCGTAAGGAGGAAGCGTG

Downstream 100 bases:

>100_bases
CCGTGACTTTATGCGTTATAGCCGCCAAATCCTGCTCGACGATATCGCTCTGGACGGGCAGCAAAAACTGCTCGACAGCC
AGGTGCTGATTATTGGTCTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQA
YGVHLGQEDLQATDLSAIRAAGLRLGVSTHDDMEIDVALAERPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE

Sequences:

>Translated_211_residues
MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQA
YGVHLGQEDLQATDLSAIRAAGLRLGVSTHDDMEIDVALAERPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE
>Mature_211_residues
MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQA
YGVHLGQEDLQATDLSAIRAAGLRLGVSTHDDMEIDVALAERPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=211, Percent_Identity=98.1042654028436, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6325042, Length=214, Percent_Identity=28.9719626168224, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23087; Mature: 23087

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGR
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
RYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAGLRLGVSTHDDMEIDVALA
CCCCEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEC
ERPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYPTVAIGGISLARAPAVIATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHCC
GSIAVVSAITQAADWRLATAQLLEIAGVGDE
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGR
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
RYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAGLRLGVSTHDDMEIDVALA
CCCCEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEC
ERPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYPTVAIGGISLARAPAVIATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHCC
GSIAVVSAITQAADWRLATAQLLEIAGVGDE
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA