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The map label for this gene is thiC [H]

Identifier: 218692275

GI number: 218692275

Start: 4653514

End: 4655409

Strand: Reverse

Name: thiC [H]

Synonym: ECED1_4701

Alternate gene names: 218692275

Gene position: 4655409-4653514 (Counterclockwise)

Preceding gene: 218692276

Following gene: 218692274

Centisome position: 89.36

GC content: 55.06

Gene sequence:

>1896_bases
ATGTCTGCAACAAAACTGACCCGCCGCGAACAACGCGCCCAGGCCCAACATTTTATCGACACCCTGGAAGGCTCCGCCTT
TCCCAACTCAAAACGCATTTACATCACTGGCACACACCCCGGCGTACGCGTGCCGATGCGTGAGATTCAGCTTAGCCCGA
CGCTAATTGGCGGCAGCAAAGAACAGCCGCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTAT
GGCGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATAC
CGAAGAACTTACCGTGCGCAGTTCCGATTACACAAAAGCGCGGCTGGCAGATGATGGCCTCGACGAGCTGCGTTTTAGCG
GCGTATTAACCCCAAAACGCGCCAAAGCAGGCCACCGCGTCACACAACTGCACTACGCCCGCAAGGGTATCATCACGCCA
GAAATGGAATTCATCGCCATTCGCGAGAATATGGGTCGCGAGCGCATACGTAGCGAAGTTTTACGCCACCAGCATCCGGG
AATGAGCTTTGGCGCACGTCTGCCGGAAAATATCACTGCGGAATTTGTCCGTGATGAAGTTGCTGCCGGACGTGCGATTA
TCCCGGCCAACATTAATCATCCGGAATCGGAGCCGATGATTATTGGCCGTAACTTCCTGGTAAAAGTTAACGCCAATATC
GGCAACTCAGCGGTCACTTCTTCCATCGAAGAAGAAGTGGAAAAACTGGTATGGTCCACACGCTGGGGAGCGGATACCGT
GATGGATCTTTCCACTGGTCGCTATATTCACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAG
TGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTG
GAACAAGCCGAGCAAGGTGTGGATTACTTCACGATCCATGCGGGGGTGCTGCTGCGCTACGTGCCGATGACCGCGAAACG
CCTGACCGGTATCGTCTCTCGCGGCGGTTCGATTATGGCGAAATGGTGCCTCTCCCATCATCAGGAAAATTTCCTCTATC
AACACTTCCGCGAAATTTGTGAAATCTGTGCTGCTTATGACGTTTCGCTATCGCTGGGCGACGGCCTGCGCCCCGGTTCT
ATTCAGGACGCCAACGATGAAGCACAGTTTGCCGAGCTGCATACGCTGGGTGAACTGACTAAAATCGCCTGGGAATATGA
CGTACAGGTGATGATTGAAGGCCCAGGCCACGTGCCGATGCAGATGATTCGCCGCAATATGACCGAGGAGTTAGAGCACT
GCCACGAAGCGCCGTTTTACACTCTGGGGCCGCTAACTACCGATATCGCGCCGGGCTATGACCACTTCACGTCAGGGATT
GGCGCGGCGATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCAAATAA
AGAAGATGTTAAACAGGGGCTTATCACCTATAAGATTGCCGCTCACGCCGCCGACCTGGCGAAAGGGCATCCGGGCGCGC
AAATTCGCGATAACGCCATGTCGAAAGCCCGCTTCGAATTTCGCTGGGAAGACCAGTTTAATCTGGCCCTCGACCCGTTT
ACTGCCCGCGCTTATCACGATGAAACCCTGTCGCAAGAGTCCGGTAAAGTCGCCCATTTTTGCTCCATGTGTGGACCGAA
ATTCTGCTCGATGAAAATCAGCCAGGAAGTGCGTGATTACGCCGCCGCGCAAACCATTGAAGTGGGAATGGCGGATATGT
CGGAGAACTTCCGCGCCAGAGGCGGAGAAATCTACCTGCGTAAGGAGGAAGCGTGA

Upstream 100 bases:

>100_bases
GGAACAAGAGTTAATCTGCTATCGCATCGCCTTTGCGGCGATCGTCTCTTGCTTCATCCGTCGTCTGACAAGCCACGTCC
TTAACTTTTTGGAACGAGCT

Downstream 100 bases:

>100_bases
TGTATCAGCCTGAGTTTCCTCCTGTACCCTTTCGTTTAGGACTGTACCCGGTGGTTGACAGCGTACAGTGGATCGAACGT
CTGTTGGATGCAGGCGTACG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPY
GDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLSQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA

Sequences:

>Translated_631_residues
MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPY
GDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLSQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA
>Mature_630_residues
SATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYG
DPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITPE
MEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIG
NSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLE
QAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSI
QDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGIG
AAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFT
ARAYHDETLSQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=631, Percent_Identity=98.5736925515055, Blast_Score=1301, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 70765; Mature: 70634

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHEEECCEEECCCC
EQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKA
CCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCH
RLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITPEMEFIAIRENMGRERIRSEV
HHCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECEEEEEEECCC
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAYHDETLSQESGKVAHFCSMCGPKFCSMKISQEVRDY
HHCEEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AAAQTIEVGMADMSENFRARGGEIYLRKEEA
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
SATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHEEECCEEECCCC
EQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKA
CCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCH
RLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITPEMEFIAIRENMGRERIRSEV
HHCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECEEEEEEECCC
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAYHDETLSQESGKVAHFCSMCGPKFCSMKISQEVRDY
HHCEEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AAAQTIEVGMADMSENFRARGGEIYLRKEEA
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA