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The map label for this gene is pflD [H]

Identifier: 218692237

GI number: 218692237

Start: 4599232

End: 4601529

Strand: Direct

Name: pflD [H]

Synonym: ECED1_4656

Alternate gene names: 218692237

Gene position: 4599232-4601529 (Clockwise)

Preceding gene: 218692236

Following gene: 218692238

Centisome position: 88.28

GC content: 53.13

Gene sequence:

>2298_bases
ATGACGAATCGTATCTCTCGCCTCAAAACTGCACTGTTTGCCAATACCCGTGAAATCTCGCTGGAGCGGGCGTTGCTTTA
TACCGCCAGCCATCGGCAAACCGAAGGGGAGCCGGTGATAATGCGTCGGGCGAAAGCAACAGCGTATATCCTTGAACACG
TTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCACGCGCCGGGATTATGTCGCCGGAA
ATGGACCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGA
TAAACGTATCTACCGCGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACGG
ATGAAGTAAAAGCCGCGACCAGCACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGAT
TACCCGCGCCTGCTGAATCACGGGCTGGGGGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCA
CTTTTATCAGGCAGCGCTGTTACTGCTGGAAGCCTCGCAGAAACATATTTTGCGTTACGCCGAACTGGCGGAAACGATGG
CGGCAAGCTGCACTGATGGCCCGCGTCGCGAAGAACTGCTGACCATTGCGGAGATCTCCCGCCATAACGCCGAACATAAG
CCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCT
ATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGGCATCATTAACCCAGGGCGAAGATCCGGCATTCCTGA
AAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGATATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCA
GGTTTCCCTACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCT
TTGCCTTGACGCTTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGT
TCCTGATGAAAACCGCAGAAACCATTCGCCTCGGCACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCG
TTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG
AACCTACGGCTTGCATGACATCGCGATGTTTAACCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAACG
CTGCGCTGACTTATGAAGGTTTACTGGAACAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGC
AATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTACTCTCGTCTTTTATCAGCGATTGTCTGGAAAAAGG
CCGCGATATTACTGACGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTC
TCCATGCGTTGAAAGGGATGATTTTTGATCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTC
GCCACGCCAGAAGGCGAAAAAGTCCGCGCTCGCTTAATTAACCGCTTCGAGAAATACGGTAACGATATCGACGAGGTGGA
TAACATCAGCGCCGAACTGTTACGCCACTACTGCAAAGAAGTGGAAAAATACCAGAACCCGCGCGGCGGCTACTTCACGC
CAGGATCGTATACCGTTTCTGCTCACGTCCCGTTGGGATCGGTGGTTGGCGCGACGCCAGACGGTCGTTTTGCCGGGGAA
CAACTGGCAGACGGTGGCTTGTCACCCATGCTGGGTCAGGATGCACAAGGGCCAACGGCGGTGCTGAAATCAGTCAGTAA
GCTCGATAATACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCGGGAT
TACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTGCAGCATATTCAGTTTAACGTGGTGAACGCCGAC
ACGTTACGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGT
CGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAA

Upstream 100 bases:

>100_bases
CTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTATCCCGCCCGCAAGGGCGGGTTCG
CTTTCCCACAGGAGTTCCTC

Downstream 100 bases:

>100_bases
CGTTGTGGAAACGCGCCGCAATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTC
GTACGGTGGTCTTTTTTAAA

Product: putative formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDGPRREELLTIAEISRHNAEHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Sequences:

>Translated_765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDGPRREELLTIAEISRHNAEHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
>Mature_764_residues
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEM
DPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDY
PRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDGPRREELLTIAEISRHNAEHKP
QTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAG
FPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAF
LNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGSN
ICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFA
TPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQ
LADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADT
LREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=98.562091503268, Blast_Score=1571, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=805, Percent_Identity=37.1428571428571, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994926, Length=641, Percent_Identity=26.0530421216849, Blast_Score=149, Evalue=9e-37,
Organism=Escherichia coli, GI1787131, Length=547, Percent_Identity=25.4113345521024, Blast_Score=141, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85843; Mature: 85712

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRAKATAYILEHVEISIRD
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDGPRREELLTIAEISRHNAEHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
IFDQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRAKATAYILEHVEISIRD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDGPRREELLTIAEISRHNAEHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
IFDQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]