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The map label for this gene is frwB

Identifier: 218692236

GI number: 218692236

Start: 4598861

End: 4599181

Strand: Direct

Name: frwB

Synonym: ECED1_4655

Alternate gene names: 218692236

Gene position: 4598861-4599181 (Clockwise)

Preceding gene: 218692235

Following gene: 218692237

Centisome position: 88.28

GC content: 49.84

Gene sequence:

>321_bases
ATGACGAAAATTATCGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACTTATATGGCGGCAGAGGCGCTGGAAAGCGC
CGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCGATTGGTCTGGAAAATGAACTGACTGCGGAAGACG
TGGCAAGCGCCGATATGGTGATTTTGACCAAAGATATCGGCATCAAATTTGAAGAGCGTTTTGCTGGCAAGACTATCGTG
CGTGTCAACATCAGCGACGCGGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTA
A

Upstream 100 bases:

>100_bases
ACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCAACTGAAGAGAAGGAAGACGACCTGGATCTGGATTTTGAAATT
AATTAATTGAGGAACGAGCC

Downstream 100 bases:

>100_bases
GTTCTTATCCCGCCCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTCCTCATGACGAATCGTATCTCTCGCCTCAAAACT
GCACTGTTTGCCAATACCCG

Product: putative PTS system fructose-like transporter subunit EIIB

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIV
RVNISDAVKRADAIMSKIEAHLAQTA

Sequences:

>Translated_106_residues
MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIV
RVNISDAVKRADAIMSKIEAHLAQTA
>Mature_105_residues
TKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIVR
VNISDAVKRADAIMSKIEAHLAQTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain

Homologues:

Organism=Escherichia coli, GI1790387, Length=106, Percent_Identity=100, Blast_Score=211, Evalue=6e-57,
Organism=Escherichia coli, GI1788492, Length=88, Percent_Identity=53.4090909090909, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI87082348, Length=98, Percent_Identity=50, Blast_Score=99, Evalue=8e-23,
Organism=Escherichia coli, GI1788730, Length=101, Percent_Identity=45.5445544554455, Blast_Score=97, Evalue=2e-22,
Organism=Escherichia coli, GI1790390, Length=98, Percent_Identity=43.8775510204082, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1786951, Length=94, Percent_Identity=43.6170212765958, Blast_Score=85, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFB2_ECO57 (P69818)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D86086
- PIR:   G91238
- RefSeq:   NP_290587.1
- RefSeq:   NP_312906.1
- ProteinModelPortal:   P69818
- SMR:   P69818
- EnsemblBacteria:   EBESCT00000026556
- EnsemblBacteria:   EBESCT00000057340
- GeneID:   915006
- GeneID:   960182
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5506
- KEGG:   ecs:ECs4879
- GeneTree:   EBGT00070000031719
- HOGENOM:   HBG564028
- OMA:   IENQLTP
- ProtClustDB:   PRK10474
- BioCyc:   ECOL83334:ECS4879-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353
- TIGRFAMs:   TIGR00829

Pfam domain/function: PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 11248; Mature: 11117

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: ACT_SITE 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMV
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCEE
ILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHLAQTA
EEEECCCCEEHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMV
CEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCEE
ILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHLAQTA
EEEECCCCEEHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796