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The map label for this gene is metR [H]

Identifier: 218692104

GI number: 218692104

Start: 4449426

End: 4450379

Strand: Reverse

Name: metR [H]

Synonym: ECED1_4513

Alternate gene names: 218692104

Gene position: 4450379-4449426 (Counterclockwise)

Preceding gene: 218692109

Following gene: 218692100

Centisome position: 85.43

GC content: 55.77

Gene sequence:

>954_bases
ATGATCGAAGTAAAACACCTGAAAACGCTACAAGCGTTGCGGAACTGCGGCTCACTCGCAGCCGCTGCGGCGACGTTGCA
TCAAACGCAATCCGCCCTGTCTCACCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAAAGCC
AGCCGCTTCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAATCAGGTGCTGCCGCAAATTAGCCAGGCACTG
CAAGCCTGCAATGAACCGCAGCAGACGCGCCTGCGCATTGCCATAGAGTGCCATAGCTGTATTCAGTGGCTGACGCCCGC
ATTAGAAAGTTTCCATAAGAACTGGCCGCAGGTGGAGATGGATTTTAAATCGGGCGTAACGTTTGATCCGCAGCCTGCGT
TGCAACAGGGCGAGCTGGATCTGGTGATGACGTCCGATATTCTGCCGCGCAGTGGCCTGCATTATTCGCCGATGTTCGAC
TATGAAGTGCGTCTGGTGTTAGCACCTGACCATCCACTGGCGGCAAAAACGCGTATTACACCGGAAGATCTCGCCAGCGA
AACGCTGTTGATTTATCCGGTGCAGCGTAGCCGACTGGATGTCTGGAGGCATTTTCTTCAGCCAGCAGGCGTCAGCCCGT
CACTGAAAAGCGTCGATAACACCTTATTGTTGATTCAGATGGTTGCCGCACGGATGGGTATTGCCGCACTACCGCATTGG
GTGGTGGAGAGTTTTGAACGCCAGGGTCTGGTGGTCACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGC
CGTGCGCGATGGCGAGCAGCGTCAGCCGATTACAGAAGCGTTTATTCGTTCAGCGCGCAATCACGCCTGCGATCATCTGC
CGTTTGTGAAGAGCGCGGAGCGACCCACTTACGATGCACCCACAGTGAGGCCAGGATCACCAGCGCGCCTGTAA

Upstream 100 bases:

>100_bases
ATTTTTAGTATTTAGCCGTCCGGATGTTTACACATCCATAATTGGCGGTTACTGTATATTCCTCAAGCGCAAATTATTCA
TGCCGAAGTGAAGGACTTTC

Downstream 100 bases:

>100_bases
TAAACGTTGGCCAGTGCGGCTGTTGGTGCCAGATAGCCAGGTTTACCAGCAGCCCTGCCGGAACGTGCATATTATTCATA
ATGCCCAGCGTTCCGGCGTC

Product: DNA-binding transcriptional activator, homocysteine-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQAL
QACNEPQQTRLRIAIECHSCIQWLTPALESFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL

Sequences:

>Translated_317_residues
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQAL
QACNEPQQTRLRIAIECHSCIQWLTPALESFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL
>Mature_317_residues
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQAL
QACNEPQQTRLRIAIECHSCIQWLTPALESFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=317, Percent_Identity=99.3690851735016, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI157672245, Length=236, Percent_Identity=24.5762711864407, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082024, Length=274, Percent_Identity=28.4671532846715, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=23.448275862069, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787530, Length=207, Percent_Identity=27.536231884058, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787879, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=219, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.843137254902, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=299, Percent_Identity=25.0836120401338, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786401, Length=245, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789173, Length=176, Percent_Identity=28.9772727272727, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786448, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=234, Percent_Identity=26.4957264957265, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789506, Length=190, Percent_Identity=24.2105263157895, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35616; Mature: 35616

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQWLTPALESFHKNWPQVEM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCEEE
DFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDYEVRLVLAPDHPLAAKTRIT
HHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
HHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPITEAFIRSARNHACDHLPFVKSAE
HHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCC
RPTYDAPTVRPGSPARL
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQWLTPALESFHKNWPQVEM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCEEE
DFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDYEVRLVLAPDHPLAAKTRIT
HHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
HHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPITEAFIRSARNHACDHLPFVKSAE
HHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCC
RPTYDAPTVRPGSPARL
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]