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The map label for this gene is aufB [H]

Identifier: 218691714

GI number: 218691714

Start: 4004345

End: 4005124

Strand: Reverse

Name: aufB [H]

Synonym: ECED1_4099

Alternate gene names: 218691714

Gene position: 4005124-4004345 (Counterclockwise)

Preceding gene: 218691717

Following gene: 218691713

Centisome position: 76.88

GC content: 38.21

Gene sequence:

>780_bases
GTGTTATTTCATCAACAAGGATGCTGTATGAAATGTATTATTGGAAATAAAAAATGGGTTTATGGATTCAGGGATGAATT
AAAAGTATCACAAAATATTATACGCTCAGGGATTCTTTTTTTACTATCGATATTATTTATTAATAATGCGTCCGCGGGCG
GGTTTGGCCTTGGCGTAACGCGATTGATTTATCCGGCAGAAAGTAAGCAAATAACACTCAGTGTGCAAAATAGCGGTGAC
AACACCAGTTATTTAATTCAATCATGGGTTGATGACAGTAAAACGGAAAAAAAGAGCCAGGATTTTGTTATCACTCCTCC
CCTCTTTATGTTACCAACGCGTAAAGAAGCTTCATTACGTATCATGTTTCTTGGTAAGACTAATTTGCCGACCGATCGTG
AAACTTTGTATTGGATGAATGTTAAAGCAATTCCGCCAACAGATGAGAAAAATACACAGAAGAATACCTTACAGTTGGCA
TTACAAAACAAAATCAAGCTATTTTATCGCCCGGAAAATCTACCTGTGCAACCAGGAAAAGCACGAAATATGTTGCGGTT
TAAGTATGAGGGCAAACAACTAAAAGTTATCAATCCTTCGCCTTATTACCTGACACTGACAGGAATTAAAGTACAAGGGA
GTAAATTGCCCAATATGTTTGTACCACCGAAAAGTGATATTACGGTCTCGTCGCCAGTGACGTTGGCTGGTGAAATTACT
TATCAAACAATTAATGACTTTGGCGCTACGACGGAGCGGCAGAAAGGAATAATGCAGTAA

Upstream 100 bases:

>100_bases
GCAAGTTTTGTGGTGCTGTACGAGTAAATATAAATACGATTTCAGGTTACAAAAAGTTGTAACCTGAGATTGAAATTATT
TAATAGAGTATGAATAACAG

Downstream 100 bases:

>100_bases
CAACGGAATAGTTCAGATGCAACACAGGTCAGGGATATGAACCTAAAGCTCAAAAGATGCGAATATTGGATGGCGGCACA
AAAGCAGATGAAACGGGTTG

Product: putative fimbrial chaperone AufB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLFHQQGCCMKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAGGFGLGVTRLIYPAESKQITLSVQNSGD
NTSYLIQSWVDDSKTEKKSQDFVITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLA
LQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDITVSSPVTLAGEIT
YQTINDFGATTERQKGIMQ

Sequences:

>Translated_259_residues
MLFHQQGCCMKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAGGFGLGVTRLIYPAESKQITLSVQNSGD
NTSYLIQSWVDDSKTEKKSQDFVITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLA
LQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDITVSSPVTLAGEIT
YQTINDFGATTERQKGIMQ
>Mature_259_residues
MLFHQQGCCMKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAGGFGLGVTRLIYPAESKQITLSVQNSGD
NTSYLIQSWVDDSKTEKKSQDFVITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLA
LQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDITVSSPVTLAGEIT
YQTINDFGATTERQKGIMQ

Specific function: Required for the biogenesis of type 1 fimbriae. Binds and interact with fimH [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1790771, Length=224, Percent_Identity=45.5357142857143, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1786743, Length=215, Percent_Identity=40.4651162790698, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787171, Length=213, Percent_Identity=43.1924882629108, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1789532, Length=217, Percent_Identity=35.9447004608295, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786333, Length=239, Percent_Identity=29.2887029288703, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=31.7972350230415, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1788677, Length=198, Percent_Identity=31.3131313131313, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788428, Length=219, Percent_Identity=32.4200913242009, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87081806, Length=227, Percent_Identity=29.0748898678414, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082203, Length=233, Percent_Identity=30.0429184549356, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 29294; Mature: 29294

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFHQQGCCMKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAGGFGLGVT
CCCCCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
RLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFMLPTRKEASLR
EEEECCCCCEEEEEEECCCCCHHHHHEECCCCCCCHHCCCCEEECCCEEEEECCCCCEEE
IMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFYRPENLPVQPGK
EEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCHHHEEEEEECCEEEEECCCCCCCCCCC
ARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDITVSSPVTLAGEIT
CCCEEEEEECCCEEEEECCCCCEEEEEEEEEECCCCCCEECCCCCCEEECCCEEEEEEEE
YQTINDFGATTERQKGIMQ
EEEHHHCCCCCHHHCCCCC
>Mature Secondary Structure
MLFHQQGCCMKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAGGFGLGVT
CCCCCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
RLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFMLPTRKEASLR
EEEECCCCCEEEEEEECCCCCHHHHHEECCCCCCCHHCCCCEEECCCEEEEECCCCCEEE
IMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFYRPENLPVQPGK
EEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCHHHEEEEEECCEEEEECCCCCCCCCCC
ARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDITVSSPVTLAGEIT
CCCEEEEEECCCEEEEECCCCCEEEEEEEEEECCCCCCEECCCCCCEEECCCEEEEEEEE
YQTINDFGATTERQKGIMQ
EEEHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]