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The map label for this gene is rtcR [H]

Identifier: 218691697

GI number: 218691697

Start: 3990079

End: 3991677

Strand: Direct

Name: rtcR [H]

Synonym: ECED1_4082

Alternate gene names: 218691697

Gene position: 3990079-3991677 (Clockwise)

Preceding gene: 218691694

Following gene: 218691701

Centisome position: 76.59

GC content: 54.85

Gene sequence:

>1599_bases
ATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGAACGGTGCTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATG
GCGTCCGACACTCTGTCTATGCCAGCAAGAATCGTTAGTTATCGATCGGCTGGAATTATTGCACGACGCCCGCTCGCGCT
CGCTATTCGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGGGCGTTGAGATAGAACTGCAT
AACCCTTGGGATTTCGAAGAGGTTTACGCCTGCCTGCATGATTTCGCCCGTGGTTACGCGTTCCAACCTGATAAAGAAGA
GTATTTAATTCACATCACCACCGGCACCCACGTCGCGCAGATTTGCTGGTTTCTGCTGGCAGAAGCGCGTTACCTGCCCA
CCCGGTTGATACAATCTTCACCACCGCGCAAAAAAGAGCGGCCCGATAGCCCTGGCGCAGTGACGATTATCGATCTCGAT
TTAAGCCGTTATAACGCCATCGCCAGCCGCTTTGCCGAGGAACGCCAGCAAACGCTTGATTTTCTTAAGTCCGGTATTGC
CACGCGTAACTCCCACTTCAACCGCATGATTGAGCAGATTGAAAAAGTGGCGATAAAATCCCGCGCACCGATCCTGCTTA
ACGGCCCGACCGGTGCAGGTAAGTCATTTCTGGCACGACGTATCTTCGAGCTAAAACAGGCGCGGCATCAGTTTAGCGGC
GCGTTTGTGGAAGTGAACTGCGCCACCCTGCGCGGCGATACCGCCATGTCGACGCTGTTTGGTCATGTGAAAGGCGCGTT
TACCGGGGCGCGGGAATCGCGTGAAGGATTATTACGCAGCGCCAACGGCGGAATGTTGTTTCTTGATGAGATTGGCGAAC
TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTG
AGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGTTGGTTGCCGAAGGCAAATTTCGTGAAGACCT
GTACGCGCGAATAAATCTCTGGACCTTCACCTTGCCAGGATTACGCCAACGCCAGGAAGATATTGAACCGAACCTGGATT
ATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGTGCCTGGCTGGCCTTT
GCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCAC
TAGCGGACGCATCACACTGGATACGGTGGAAGATGAGATCAACCGTCTGCGCTATAACTGGCAGGAGAGTCGCCCCTCCA
CGCTTACGGCGTTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACACGTTATCGCTATC
TGCCGCCAGGCAAAGTCGCTTTCTGCTGCCGGACGTGAACTGTTTGACATTTCGCGCCAGGGTAAAGCCAGCGTCAATGA
CGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACATGGGAAGCAATGCAGGATCAGCACAGCTCCAGTTGA

Upstream 100 bases:

>100_bases
TTGTTTTTAAAAATATTATATTTTTACGTCCGTAAACGGAGATTTCCCGCAAAGCCGATTTACCGATAATGAAATCTCGT
CTTTTATAAGGATATCTAAG

Downstream 100 bases:

>100_bases
ATATGATGGTCCTTCAGCACCTGCATCACGCTCGCAGGCGGCATCGTGTCGGTGTAGACAGCATCGACCATGCTGATGCT
ACCCATATTGACCATTGCGT

Product: sigma 54-dependent transcriptional regulator of rtcBA expression

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLHDARSRSLFETLKRDIASVSPETEVVGVEIELH
NPWDFEEVYACLHDFARGYAFQPDKEEYLIHITTGTHVAQICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLD
LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAI
CRQAKSLSAAGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS

Sequences:

>Translated_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLHDARSRSLFETLKRDIASVSPETEVVGVEIELH
NPWDFEEVYACLHDFARGYAFQPDKEEYLIHITTGTHVAQICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLD
LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAI
CRQAKSLSAAGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS
>Mature_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLHDARSRSLFETLKRDIASVSPETEVVGVEIELH
NPWDFEEVYACLHDFARGYAFQPDKEEYLIHITTGTHVAQICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLD
LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAI
CRQAKSLSAAGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS

Specific function: Transcriptional repressor of the rtcAB genes. Interacts with sigma-54 [H]

COG id: COG4650

COG function: function code KT; Sigma54-dependent transcription regulator containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789828, Length=532, Percent_Identity=96.9924812030075, Blast_Score=1066, Evalue=0.0,
Organism=Escherichia coli, GI1788905, Length=275, Percent_Identity=35.6363636363636, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1789233, Length=246, Percent_Identity=37.3983739837398, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1788550, Length=232, Percent_Identity=40.0862068965517, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790437, Length=256, Percent_Identity=39.0625, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI87082117, Length=258, Percent_Identity=36.4341085271318, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789087, Length=254, Percent_Identity=36.2204724409449, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787583, Length=244, Percent_Identity=35.655737704918, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI87082152, Length=238, Percent_Identity=36.9747899159664, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790299, Length=241, Percent_Identity=35.6846473029046, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786524, Length=239, Percent_Identity=35.9832635983264, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87081872, Length=257, Percent_Identity=31.9066147859922, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081858, Length=249, Percent_Identity=28.9156626506024, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078
- InterPro:   IPR009715
- InterPro:   IPR017183 [H]

Pfam domain/function: PF06956 RtcR; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60304; Mature: 60304

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLHDARSRSLFETLK
CCCEEEHHHHHHHHHHCCCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQPDKEEYLIHITTGTHVAQ
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLDLSRYNAIASRFAEERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHCCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHH
RMATFATSGRITLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAI
HHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLHDARSRSLFETLK
CCCEEEHHHHHHHHHHCCCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQPDKEEYLIHITTGTHVAQ
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLDLSRYNAIASRFAEERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHCCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHH
RMATFATSGRITLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAI
HHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3045764; 7984428; 7920643; 9738023 [H]