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The map label for this gene is insA [H]

Identifier: 218691097

GI number: 218691097

Start: 3383130

End: 3383402

Strand: Direct

Name: insA [H]

Synonym: ECED1_3437

Alternate gene names: 218691097

Gene position: 3383130-3383402 (Clockwise)

Preceding gene: 218691091

Following gene: 218691098

Centisome position: 64.94

GC content: 47.99

Gene sequence:

>273_bases
ATGGCCAGCGTTAATATTCATTGTCCCCGTTGTCAGTCAGCTCAGGTTTACCGCCATGGTCAGAACCCTAAAGGCCGTGA
CAGATTTCGCTGCCGTGACTGCCACCGTGTGTTTCAGCTCACTTATACTTATCAAGCACGTAAGCCGGGTATGAAAGAGA
TGATCACTGAAATGGCCTTTAATGGTGCCGGGGTTCGCGATACCGCCAGGACACTGAAAATTGGTATTAACACCATCATC
CGGACTTTAAAAAACTCGCACCAACGCAAATAA

Upstream 100 bases:

>100_bases
AACCCTTGCTATATGTAATCAAAGTCCTACGAATGTAGGAAAGGGTGATGCCTCTAATTGCTTGAATCTGATGTATAATA
CGGGCTTTTGAGGTTATCTC

Downstream 100 bases:

>100_bases
CTTCTTCGCCTGTTGCCCATGCTGATGTGGCGCTTATCTGCGAGCTTGATGAGCAATGGAGCTACGTTGGCAGTAAAGCC
CGGCTACACTGGCTCTGGTA

Product: transposase InsA (ORF1) IS1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MASVNIHCPRCQSAQVYRHGQNPKGRDRFRCRDCHRVFQLTYTYQARKPGMKEMITEMAFNGAGVRDTARTLKIGINTII
RTLKNSHQRK

Sequences:

>Translated_90_residues
MASVNIHCPRCQSAQVYRHGQNPKGRDRFRCRDCHRVFQLTYTYQARKPGMKEMITEMAFNGAGVRDTARTLKIGINTII
RTLKNSHQRK
>Mature_89_residues
ASVNIHCPRCQSAQVYRHGQNPKGRDRFRCRDCHRVFQLTYTYQARKPGMKEMITEMAFNGAGVRDTARTLKIGINTIIR
TLKNSHQRK

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=87, Percent_Identity=47.1264367816092, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI1788204, Length=87, Percent_Identity=47.1264367816092, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI1786204, Length=87, Percent_Identity=47.1264367816092, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI87081814, Length=87, Percent_Identity=47.1264367816092, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786471, Length=87, Percent_Identity=47.1264367816092, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786460, Length=87, Percent_Identity=47.1264367816092, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1790747, Length=87, Percent_Identity=43.6781609195402, Blast_Score=87, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10465; Mature: 10334

Theoretical pI: Translated: 11.28; Mature: 11.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVNIHCPRCQSAQVYRHGQNPKGRDRFRCRDCHRVFQLTYTYQARKPGMKEMITEMAF
CCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NGAGVRDTARTLKIGINTIIRTLKNSHQRK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASVNIHCPRCQSAQVYRHGQNPKGRDRFRCRDCHRVFQLTYTYQARKPGMKEMITEMAF
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NGAGVRDTARTLKIGINTIIRTLKNSHQRK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]