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The map label for this gene is insB [H]

Identifier: 218691098

GI number: 218691098

Start: 3383429

End: 3383788

Strand: Direct

Name: insB [H]

Synonym: ECED1_3438

Alternate gene names: 218691098

Gene position: 3383429-3383788 (Clockwise)

Preceding gene: 218691097

Following gene: 218691099

Centisome position: 64.95

GC content: 52.5

Gene sequence:

>360_bases
GTGGCGCTTATCTGCGAGCTTGATGAGCAATGGAGCTACGTTGGCAGTAAAGCCCGGCTACACTGGCTCTGGTACGCGTA
CAACACCAAAACAGGCGGTGTACTGGCCTACACTTTTGGTCCCCGAACCGATCAAACGTGCCGGGAGCTACTGGCACTGC
TTACACCCTTCAACATCGGCATACTCACCAGCGATGACTGGGACAGCTATGGCCGGGTGGTGCCGAAGAATAAGCATCTG
ACCGGCAAAATATTCATCCAACGCATTGAGCGTAATAATCTGACGCTACGCACCCACATTAAGCGGTTGGCTCGTAAACA
ATCTGCTTCTCACGCTCAGTTGAGATCCACGAAAAAGTGA

Upstream 100 bases:

>100_bases
ATACCGCCAGGACACTGAAAATTGGTATTAACACCATCATCCGGACTTTAAAAAACTCGCACCAACGCAAATAACTTCTT
CGCCTGTTGCCCATGCTGAT

Downstream 100 bases:

>100_bases
TCGGAGCATTTATCGAAAAACACATATTCTACTAATTGGATGCACTACCGAGATTTGAATCAAAGCGTTGGTATGCAAGG
GATGTGTCGAACTCTCTAGT

Product: transposase InsB (ORF2) IS1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MALICELDEQWSYVGSKARLHWLWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGILTSDDWDSYGRVVPKNKHL
TGKIFIQRIERNNLTLRTHIKRLARKQSASHAQLRSTKK

Sequences:

>Translated_119_residues
MALICELDEQWSYVGSKARLHWLWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGILTSDDWDSYGRVVPKNKHL
TGKIFIQRIERNNLTLRTHIKRLARKQSASHAQLRSTKK
>Mature_118_residues
ALICELDEQWSYVGSKARLHWLWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGILTSDDWDSYGRVVPKNKHLT
GKIFIQRIERNNLTLRTHIKRLARKQSASHAQLRSTKK

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=106, Percent_Identity=45.2830188679245, Blast_Score=100, Evalue=2e-23,
Organism=Escherichia coli, GI1788203, Length=106, Percent_Identity=45.2830188679245, Blast_Score=100, Evalue=2e-23,
Organism=Escherichia coli, GI1786203, Length=106, Percent_Identity=45.2830188679245, Blast_Score=100, Evalue=2e-23,
Organism=Escherichia coli, GI1786470, Length=106, Percent_Identity=45.2830188679245, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1786459, Length=106, Percent_Identity=45.2830188679245, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1787223, Length=106, Percent_Identity=45.2830188679245, Blast_Score=98, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 13792; Mature: 13661

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALICELDEQWSYVGSKARLHWLWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
CEEEEECCCHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCEE
ILTSDDWDSYGRVVPKNKHLTGKIFIQRIERNNLTLRTHIKRLARKQSASHAQLRSTKK
EEECCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure 
ALICELDEQWSYVGSKARLHWLWYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
EEEEECCCHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCEE
ILTSDDWDSYGRVVPKNKHLTGKIFIQRIERNNLTLRTHIKRLARKQSASHAQLRSTKK
EEECCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]