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The map label for this gene is hupB

Identifier: 218688304

GI number: 218688304

Start: 482173

End: 482445

Strand: Direct

Name: hupB

Synonym: ECED1_0464

Alternate gene names: 218688304

Gene position: 482173-482445 (Clockwise)

Preceding gene: 218688303

Following gene: 218688305

Centisome position: 9.26

GC content: 49.45

Gene sequence:

>273_bases
GTGAATAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGC
TATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTCAAAG
AGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGT
GCAGGTAAAGCACTGAAAGACGCGGTAAACTAA

Upstream 100 bases:

>100_bases
TAAGGGGTGTTGTAAGGGGATGGCTGGCCTGATATAACTGCTGCGCGTTCGTACCTTGAAGGATTCAAGTGCGATATAAA
TTATAAAGAGGAAGAGAAGA

Downstream 100 bases:

>100_bases
GCGTTGTCCCCAGTGGGGATGTGACGAAGTTCAAGGGCGCATCTACTGATGTGCCTTTTTTATTTGTATTCGGTGACTTT
CTGCGTCTTGTGGGCTGACA

Product: transcriptional regulator HU subunit beta

Products: NA

Alternate protein names: HU-1; NS1

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFR
AGKALKDAVN

Sequences:

>Translated_90_residues
MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFR
AGKALKDAVN
>Mature_90_residues
MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFR
AGKALKDAVN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=100, Blast_Score=174, Evalue=9e-46,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=69.6629213483146, Blast_Score=123, Evalue=3e-30,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=35.1648351648352, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1788005, Length=84, Percent_Identity=36.9047619047619, Blast_Score=63, Evalue=3e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): DBHB_ECO57 (P0ACF6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85541
- PIR:   F90690
- RefSeq:   NP_286182.1
- RefSeq:   NP_308521.1
- ProteinModelPortal:   P0ACF6
- SMR:   P0ACF6
- MINT:   MINT-1236737
- PRIDE:   P0ACF6
- EnsemblBacteria:   EBESCT00000025838
- EnsemblBacteria:   EBESCT00000059665
- GeneID:   914596
- GeneID:   957410
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0547
- KEGG:   ecs:ECs0494
- GeneTree:   EBGT00050000010314
- HOGENOM:   HBG705085
- OMA:   NESKTHF
- ProtClustDB:   PRK10664
- BioCyc:   ECOL83334:ECS0494-MONOMER
- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992
- Gene3D:   G3DSA:4.10.520.10
- PRINTS:   PR01727
- SMART:   SM00411

Pfam domain/function: PF00216 Bac_DNA_binding; SSF47729 IHF_like_DNA_bnd

EC number: NA

Molecular weight: Translated: 9226; Mature: 9226

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCC
RNPQTGKEITIAAAKVPSFRAGKALKDAVN
CCCCCCCEEEEEECCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCC
RNPQTGKEITIAAAKVPSFRAGKALKDAVN
CCCCCCCEEEEEECCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796