Click here to switch to the map view.

The map label for this gene is glgX

Identifier: 218550697

GI number: 218550697

Start: 3474924

End: 3476900

Strand: Reverse

Name: glgX

Synonym: EFER_3408

Alternate gene names: 218550697

Gene position: 3476900-3474924 (Counterclockwise)

Preceding gene: 218550698

Following gene: 218550696

Centisome position: 75.77

GC content: 51.09

Gene sequence:

>1977_bases
ATGACGCAACTGACAACCGGCAAGGCGACGCCTCATGGTGCAACGTATGACGGGCATGGCGTTAACTTTACACTCTTTTC
TGCTCATGCCGAACGCGTAGAACTGTGTGTCTTTGATGCACAGGGAAATGAGGTACGTTTTGACCTGCCGGGGCGCAGCG
GAGATGTCTGGCATGGCTATTTAGCAGATGCCAGACCCGGTTTGCGCTATGGTTATCGTGTACATGGTCCGTGGCAACCT
GCCGCAGGGCATCGCTTTAACCCGGCGAAACTGTTGATTGATCCCTGCGCACGTCGTGTTGAAGGTGATCTTCATGATAA
CCCGCTATTCCACGCGGGCTATGAAACACCAGATAGCCGGGATAACGCCACCATCGCACCAAAATGTGTGGTGGTGTCTG
ATAGTTATAACTGGGAAGACGACGCCCCGCCGCGCACGCCGTGGGGCAATACGGTCATCTACGAAGCCCATGTAAAAGGC
TTAACGTACCAGCACCCGGAAATCCCGGCAGAGATCCGTGGGCGGTATAAAGCGTTGGGCCATCCGGTAATGATCAACTA
TTTCAAACGGTTGGGGATTACCGCGCTGGAGCTGATGCCTGTTATCCATTTTGCCAGCGAACCGCGTCTGCAACGACTCG
GATTGACAAACTATTGGGGCTATAACCCGATGGCGATGTTTGCGCTGCATCCGGTGTATGCCTGTTCGCCAGAAAGTGCG
CTGGATGAGTTCCGGGATGCCGTTAAAGAGCTGCATAAAGCAGGAATCGAAGTCATTCTCGATATCGTCATCAACCATAG
TGCAGAGCTGGATCTCGACGGGCCGATGTTCTCACTGCGCGGAATTGACAACCGTAGCTATTATTGGATCAAAGAGGATG
GCGATTATCACAACTGGACGGGTTGTGGTAATACGCTGAATCTGAGTAATCCTGGCGTGATGGAATATGCGCTCGAATGC
CTGCGTTACTGGGTTGAAACCTGCCATGTTGATGGTTTTCGTTTTGATTTGGCATCGGTAATGGGGCGCACTCCGGCGTT
TCGCCAGGACGCACCACTTTTTACTGCTATCCAGAACTGCCCTGTACTTTCGCAGGTGAAGCTTATCGCCGAACCGTGGG
ATATCGGCGAAGGTGGATATCAGGTGGGAAATTTCCCACCGTTGTTTGCGGAGTGGAATGATCACTTCCGCGATGCAACC
CGGCGTTTCTGGTTGCAACGTAATTTGTCACTGGGTGATTTTGCGGGGCGTTTTGCTGGCTCCAGCGACGTATTTAATCG
TAACGGTCGTTTGCCAGGCGCATCAATAAATTTGGTCACTGCGCATGACGGTTTCACGTTACGTGACTGTGTCTGCTTCA
CAAATAAACACAATGAAGCAAATGGAGAAGAAAATCGCGACGGCAGCAACAATAACCATAGTAACAATCATGGTAAAGAA
GGATTAGGCGGATCACTTGATCTGATTGAGCGTCGACGCGACAGCGTACACGCGCTGTTAACGACGCTTTTGCTTTCACA
GGGAACGCCAATGCTGCTGGCAGGTGATGAACATGGCCATAGCCAGCATGGAAACAATAACGCGTATTGTCAGGATAATG
CGCTGACCTGGCTGGACTGGGAGCGGGCAAACAATGGATTGACCGATTTTACAGCGGCATTGATTCATCTACGGCAACAA
ATTCCGGCACTGACACGAAATCAGTGGTGGCAAGAGAATGATGGCAATGTACGTTGGTTAAATAAAGATGGTCAGGTTTT
AAGTGTTGAAGAGTGGCAACATGGTATGACATGTATGCAAATCATGTTATCCGACCGCTGGCTTATCGCACTGAATGCTA
CACCACATTTGGTTGATATTGTTCTGCCCGAGGGGGAATGGCGCGCTATTCCTCCTTTCGCCGGAGAGGATAATCCGGTC
GTTTTGGAAGTCTGGCATGGGTCTGCGCACGGGCTGTGCGTGTTTCAAAAGTCATAG

Upstream 100 bases:

>100_bases
GCGTGGTACAGAGCGATGAGATCGCCAGCCACGGACGTCAGCACTCTCTGAGCATCACACTGCCACCGCTGGCTACCGTC
TGGCTGGTTCGGGAGGGGGA

Downstream 100 bases:

>100_bases
AAAAAGGAGTTAATTATGGTTGGGCTAGAAAAAAATGATCCGCTGATGTTGGCACGCCAGCTGCCCATCAAATCTGTTGC
GCTCATTCTCGCAGGGGGTC

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MTQLTTGKATPHGATYDGHGVNFTLFSAHAERVELCVFDAQGNEVRFDLPGRSGDVWHGYLADARPGLRYGYRVHGPWQP
AAGHRFNPAKLLIDPCARRVEGDLHDNPLFHAGYETPDSRDNATIAPKCVVVSDSYNWEDDAPPRTPWGNTVIYEAHVKG
LTYQHPEIPAEIRGRYKALGHPVMINYFKRLGITALELMPVIHFASEPRLQRLGLTNYWGYNPMAMFALHPVYACSPESA
LDEFRDAVKELHKAGIEVILDIVINHSAELDLDGPMFSLRGIDNRSYYWIKEDGDYHNWTGCGNTLNLSNPGVMEYALEC
LRYWVETCHVDGFRFDLASVMGRTPAFRQDAPLFTAIQNCPVLSQVKLIAEPWDIGEGGYQVGNFPPLFAEWNDHFRDAT
RRFWLQRNLSLGDFAGRFAGSSDVFNRNGRLPGASINLVTAHDGFTLRDCVCFTNKHNEANGEENRDGSNNNHSNNHGKE
GLGGSLDLIERRRDSVHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWERANNGLTDFTAALIHLRQQ
IPALTRNQWWQENDGNVRWLNKDGQVLSVEEWQHGMTCMQIMLSDRWLIALNATPHLVDIVLPEGEWRAIPPFAGEDNPV
VLEVWHGSAHGLCVFQKS

Sequences:

>Translated_658_residues
MTQLTTGKATPHGATYDGHGVNFTLFSAHAERVELCVFDAQGNEVRFDLPGRSGDVWHGYLADARPGLRYGYRVHGPWQP
AAGHRFNPAKLLIDPCARRVEGDLHDNPLFHAGYETPDSRDNATIAPKCVVVSDSYNWEDDAPPRTPWGNTVIYEAHVKG
LTYQHPEIPAEIRGRYKALGHPVMINYFKRLGITALELMPVIHFASEPRLQRLGLTNYWGYNPMAMFALHPVYACSPESA
LDEFRDAVKELHKAGIEVILDIVINHSAELDLDGPMFSLRGIDNRSYYWIKEDGDYHNWTGCGNTLNLSNPGVMEYALEC
LRYWVETCHVDGFRFDLASVMGRTPAFRQDAPLFTAIQNCPVLSQVKLIAEPWDIGEGGYQVGNFPPLFAEWNDHFRDAT
RRFWLQRNLSLGDFAGRFAGSSDVFNRNGRLPGASINLVTAHDGFTLRDCVCFTNKHNEANGEENRDGSNNNHSNNHGKE
GLGGSLDLIERRRDSVHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWERANNGLTDFTAALIHLRQQ
IPALTRNQWWQENDGNVRWLNKDGQVLSVEEWQHGMTCMQIMLSDRWLIALNATPHLVDIVLPEGEWRAIPPFAGEDNPV
VLEVWHGSAHGLCVFQKS
>Mature_657_residues
TQLTTGKATPHGATYDGHGVNFTLFSAHAERVELCVFDAQGNEVRFDLPGRSGDVWHGYLADARPGLRYGYRVHGPWQPA
AGHRFNPAKLLIDPCARRVEGDLHDNPLFHAGYETPDSRDNATIAPKCVVVSDSYNWEDDAPPRTPWGNTVIYEAHVKGL
TYQHPEIPAEIRGRYKALGHPVMINYFKRLGITALELMPVIHFASEPRLQRLGLTNYWGYNPMAMFALHPVYACSPESAL
DEFRDAVKELHKAGIEVILDIVINHSAELDLDGPMFSLRGIDNRSYYWIKEDGDYHNWTGCGNTLNLSNPGVMEYALECL
RYWVETCHVDGFRFDLASVMGRTPAFRQDAPLFTAIQNCPVLSQVKLIAEPWDIGEGGYQVGNFPPLFAEWNDHFRDATR
RFWLQRNLSLGDFAGRFAGSSDVFNRNGRLPGASINLVTAHDGFTLRDCVCFTNKHNEANGEENRDGSNNNHSNNHGKEG
LGGSLDLIERRRDSVHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWERANNGLTDFTAALIHLRQQI
PALTRNQWWQENDGNVRWLNKDGQVLSVEEWQHGMTCMQIMLSDRWLIALNATPHLVDIVLPEGEWRAIPPFAGEDNPVV
LEVWHGSAHGLCVFQKS

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=82.3439878234399, Blast_Score=1152, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGX_ESCF3 (B7LSE2)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002384488.1
- EnsemblBacteria:   EBESCT00000126204
- GeneID:   7123118
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_3408
- GeneTree:   EBGT00050000009924
- HOGENOM:   HBG350946
- ProtClustDB:   PRK03705
- BioCyc:   EFER585054:EFER_3408-MONOMER
- HAMAP:   MF_01248
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR02100

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73976; Mature: 73844

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: ACT_SITE 336-336 ACT_SITE 371-371

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLTTGKATPHGATYDGHGVNFTLFSAHAERVELCVFDAQGNEVRFDLPGRSGDVWHGY
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCEEEEE
LADARPGLRYGYRVHGPWQPAAGHRFNPAKLLIDPCARRVEGDLHDNPLFHAGYETPDSR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
DNATIAPKCVVVSDSYNWEDDAPPRTPWGNTVIYEAHVKGLTYQHPEIPAEIRGRYKALG
CCCEECCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCEECCCCCCHHHHHHHHHCC
HPVMINYFKRLGITALELMPVIHFASEPRLQRLGLTNYWGYNPMAMFALHPVYACSPESA
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEHHHCCCEEECCCHHH
LDEFRDAVKELHKAGIEVILDIVINHSAELDLDGPMFSLRGIDNRSYYWIKEDGDYHNWT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCC
GCGNTLNLSNPGVMEYALECLRYWVETCHVDGFRFDLASVMGRTPAFRQDAPLFTAIQNC
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCC
PVLSQVKLIAEPWDIGEGGYQVGNFPPLFAEWNDHFRDATRRFWLQRNLSLGDFAGRFAG
CCHHHHHHEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
SSDVFNRNGRLPGASINLVTAHDGFTLRDCVCFTNKHNEANGEENRDGSNNNHSNNHGKE
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGSLDLIERRRDSVHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEH
ERANNGLTDFTAALIHLRQQIPALTRNQWWQENDGNVRWLNKDGQVLSVEEWQHGMTCMQ
HHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEECCCCCEEEHHHHHCHHHHHH
IMLSDRWLIALNATPHLVDIVLPEGEWRAIPPFAGEDNPVVLEVWHGSAHGLCVFQKS
HHHCCCEEEEECCCCCEEEEEECCCCCEECCCCCCCCCCEEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
TQLTTGKATPHGATYDGHGVNFTLFSAHAERVELCVFDAQGNEVRFDLPGRSGDVWHGY
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCEEEEE
LADARPGLRYGYRVHGPWQPAAGHRFNPAKLLIDPCARRVEGDLHDNPLFHAGYETPDSR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
DNATIAPKCVVVSDSYNWEDDAPPRTPWGNTVIYEAHVKGLTYQHPEIPAEIRGRYKALG
CCCEECCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCEECCCCCCHHHHHHHHHCC
HPVMINYFKRLGITALELMPVIHFASEPRLQRLGLTNYWGYNPMAMFALHPVYACSPESA
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEHHHCCCEEECCCHHH
LDEFRDAVKELHKAGIEVILDIVINHSAELDLDGPMFSLRGIDNRSYYWIKEDGDYHNWT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCC
GCGNTLNLSNPGVMEYALECLRYWVETCHVDGFRFDLASVMGRTPAFRQDAPLFTAIQNC
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCC
PVLSQVKLIAEPWDIGEGGYQVGNFPPLFAEWNDHFRDATRRFWLQRNLSLGDFAGRFAG
CCHHHHHHEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
SSDVFNRNGRLPGASINLVTAHDGFTLRDCVCFTNKHNEANGEENRDGSNNNHSNNHGKE
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGSLDLIERRRDSVHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEH
ERANNGLTDFTAALIHLRQQIPALTRNQWWQENDGNVRWLNKDGQVLSVEEWQHGMTCMQ
HHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEECCCCCEEEHHHHHCHHHHHH
IMLSDRWLIALNATPHLVDIVLPEGEWRAIPPFAGEDNPVVLEVWHGSAHGLCVFQKS
HHHCCCEEEEECCCCCEEEEEECCCCCEECCCCCCCCCCEEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA