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The map label for this gene is ddlA [H]

Identifier: 218549957

GI number: 218549957

Start: 2712312

End: 2713406

Strand: Direct

Name: ddlA [H]

Synonym: EFER_2642

Alternate gene names: 218549957

Gene position: 2712312-2713406 (Clockwise)

Preceding gene: 218549952

Following gene: 218549959

Centisome position: 59.11

GC content: 48.13

Gene sequence:

>1095_bases
ATGGAAAAATTGCGAGTTGGAATCGTATTTGGCGGAAAATCAGCGGAACACGAAGTGTCATTGCAATCGGCAAAAAATAT
TGTCGATGCCATCGACAAAACCCGCTTTGACGTCGTGCTGTTAGGTATTGATAAGCAAGGGCAATGGCACGTCAGTGATG
CGGCAAACTATTTATTGAACGCAGACGATCCGGCACGGATTGCGCTGCGTCCTTCAGCCACCAGCCTGGCACAGGTTCCG
GGCAAACATGAGCAGCAATTGATCGATGCGCAAACTGCGCAGCCGCTGCCGACGGTCGACGTGATCTTCCCTATCGTTCA
TGGCACGCTTGGTGAAGACGGTTCATTGCAGGGTATGTTGCGTGTGGCAAATCTGCCATTTGTCGGTTCTGATGTGCTTG
GTTCCGCTGCCTGCATGGATAAAGATGTCACTAAACGTTTACTACGCGATGCCGGACTTAACATCGCGCCGTTCGTCACG
CTGACTCGTGCTAACCGCAGTACCATCACCTTTGCTGAAATTGAAGCACAACTGGGGCTTCCGTTATTTGTTAAACCGGC
TAACCAGGGGTCTTCTGTCGGTGTCAGTAAAGTTACCAACGAAGCCGAATATAAAAAAGCCGTCGATTTGGCCTTTGAAT
ACGATCATAAAGTGGTTGTTGAGAAAGGTATTAAAGGACGTGAAATCGAATGTGCCGTGTTAGGTAATGATCATCCACAA
GCCAGTACCTGTGGCGAAATTGTTCTTAACAGCGATTTCTATGCCTACGACACCAAATACATTGACGATGATGGCGCGAA
AGTTGTTGTTCCGGCGGCTATCCCCGCTGAAATTAACGATAAGATCCGTGAAATTGCAGTAAAAGCCTATCAGACTCTTG
GTTGTGCAGGTATGGCACGCGTTGATGTCTTTTTAACGCTGGAAAACGACGTGGTGATTAATGAGATCAATACGTTGCCT
GGCTTTACCAACATCAGCATGTATCCGAAACTGTGGCAGGCAAGTGGTTTGAGTTATACCGACCTGATTACTCGCCTGAT
TGAGTTAGCGCTGGAGCGCCACGCAGCAAATAACGCGCTGAAAACGACAATGTAA

Upstream 100 bases:

>100_bases
CAATACAAAAATCCTCCGCAAAAACGGCTTACGGCTGGGGAATCCCGCTTTTCAACGTGGTAATGTATACGCTTTGCGTT
CCTTGGAATGGGTTGAGAAA

Downstream 100 bases:

>100_bases
TTTTTTTCGCCCGGTAACACCTGTTACCGGGCTTTTTCCTCTTCATCCACACGGCGGCGGATAATCATTCCTGCCACCCA
GAAACTGATAACCCAGGTTA

Product: D-alanine-D-alanine ligase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKQGQWHVSDAANYLLNADDPARIALRPSATSLAQVP
GKHEQQLIDAQTAQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLGSAACMDKDVTKRLLRDAGLNIAPFVT
LTRANRSTITFAEIEAQLGLPLFVKPANQGSSVGVSKVTNEAEYKKAVDLAFEYDHKVVVEKGIKGREIECAVLGNDHPQ
ASTCGEIVLNSDFYAYDTKYIDDDGAKVVVPAAIPAEINDKIREIAVKAYQTLGCAGMARVDVFLTLENDVVINEINTLP
GFTNISMYPKLWQASGLSYTDLITRLIELALERHAANNALKTTM

Sequences:

>Translated_364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKQGQWHVSDAANYLLNADDPARIALRPSATSLAQVP
GKHEQQLIDAQTAQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLGSAACMDKDVTKRLLRDAGLNIAPFVT
LTRANRSTITFAEIEAQLGLPLFVKPANQGSSVGVSKVTNEAEYKKAVDLAFEYDHKVVVEKGIKGREIECAVLGNDHPQ
ASTCGEIVLNSDFYAYDTKYIDDDGAKVVVPAAIPAEINDKIREIAVKAYQTLGCAGMARVDVFLTLENDVVINEINTLP
GFTNISMYPKLWQASGLSYTDLITRLIELALERHAANNALKTTM
>Mature_364_residues
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKQGQWHVSDAANYLLNADDPARIALRPSATSLAQVP
GKHEQQLIDAQTAQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLGSAACMDKDVTKRLLRDAGLNIAPFVT
LTRANRSTITFAEIEAQLGLPLFVKPANQGSSVGVSKVTNEAEYKKAVDLAFEYDHKVVVEKGIKGREIECAVLGNDHPQ
ASTCGEIVLNSDFYAYDTKYIDDDGAKVVVPAAIPAEINDKIREIAVKAYQTLGCAGMARVDVFLTLENDVVINEINTLP
GFTNISMYPKLWQASGLSYTDLITRLIELALERHAANNALKTTM

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=90.9340659340659, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1786280, Length=351, Percent_Identity=34.4729344729345, Blast_Score=188, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39401; Mature: 39401

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKQGQWHVSDAANYLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCEECCCHHHEEEC
ADDPARIALRPSATSLAQVPGKHEQQLIDAQTAQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLGSAACMDKDVTKRLLRDAGLNIAPFVTLTRANRSTITFAEIEAQLGL
EEECCCEECHHHHCHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEEEHHHCCC
PLFVKPANQGSSVGVSKVTNEAEYKKAVDLAFEYDHKVVVEKGIKGREIECAVLGNDHPQ
EEEEEECCCCCCCCHHHHCCHHHHHHHHHEEEECCCEEEEECCCCCCEEEEEEECCCCCC
ASTCGEIVLNSDFYAYDTKYIDDDGAKVVVPAAIPAEINDKIREIAVKAYQTLGCAGMAR
CCCCCCEEECCCEEEEECEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEE
VDVFLTLENDVVINEINTLPGFTNISMYPKLWQASGLSYTDLITRLIELALERHAANNAL
EEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
KTTM
CCCC
>Mature Secondary Structure
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKQGQWHVSDAANYLLN
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCEECCCHHHEEEC
ADDPARIALRPSATSLAQVPGKHEQQLIDAQTAQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLGSAACMDKDVTKRLLRDAGLNIAPFVTLTRANRSTITFAEIEAQLGL
EEECCCEECHHHHCHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEEEHHHCCC
PLFVKPANQGSSVGVSKVTNEAEYKKAVDLAFEYDHKVVVEKGIKGREIECAVLGNDHPQ
EEEEEECCCCCCCCHHHHCCHHHHHHHHHEEEECCCEEEEECCCCCCEEEEEEECCCCCC
ASTCGEIVLNSDFYAYDTKYIDDDGAKVVVPAAIPAEINDKIREIAVKAYQTLGCAGMAR
CCCCCCEEECCCEEEEECEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEE
VDVFLTLENDVVINEINTLPGFTNISMYPKLWQASGLSYTDLITRLIELALERHAANNAL
EEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
KTTM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]