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The map label for this gene is kstD [H]

Identifier: 218549908

GI number: 218549908

Start: 2662487

End: 2664259

Strand: Direct

Name: kstD [H]

Synonym: EFER_2593

Alternate gene names: 218549908

Gene position: 2662487-2664259 (Clockwise)

Preceding gene: 218549907

Following gene: 218549909

Centisome position: 58.02

GC content: 52.62

Gene sequence:

>1773_bases
TTGTGTTATCCAGACCGGGCCAGAAATGGCACTGGCACAAATGAAACTGATGGGCGAATTTATCGGAGCGATGCCTCAAC
AGGAAGAGGCCGCAGCATGAAAAGCTGGGATGTCGTTGTCATTGGTAGCGGCGCAGCAGGCTTTGCCGCTGCCGTTTCCG
CCTGCTGTAAAGGGCTTTCAGTACTGATGCTGGAAAAAGCAGAGCAGTTTGGTGGAACGTCAGCAATTTCAGGCGGCGCG
GTATGGCTTCACGATACCGACCAGGCGAGAGAGCTGGGGAAAAATGATTCTGCCGAGGCAATAAAAACCTATCTGCAAGC
CATTGTTGGTCCTGAAAACTACCGGGCAGAAATGATAGACGCTTTTGTGGCTCAGGGCCGTGAAGCACTGGCATTTCTTG
AAAGCGAAGGAGCTGTTAAATACAGTTTGCGTCCTTTATCCCCCGATTACTATCCCGATGAAGCTGGCGCGGTAGATGTT
GGCCGGGCGCTGGAAGTCATCGAATTTGACGGGCGCGCCCTGGGTACTCGCTTTGCTGACTTACGTTCGCCGCCACCGGG
AATGTTGCTGTTCGGTGGAATGATGGTTAACCGTGTCGATATCCAGCATTTTCTCGATATGCGCCGCTCGCTTCACTCCT
TGCTGCACTGTAGCAAATTATTACTTCGCTATGGTCGCGACCGCCTGCGTTATCACCGGGGAACACGCCTTGCAATGGGT
AATGCACTTATTGCTCGTATGGCTCATGTGGCTTTTCGTAAGGGACTAACACTGGAGCTTAACGTCAATGTTTTATCTTT
AATCGCGGAAAACGGCACCGTTACCGGCGTGCAAATTGAACAACATGGCCAGCAACACATTGTTCATGCCCGCTATGGTG
TAGTGCTCGCCGCTGGAGGGTTTGCTGCTGGTGAACTGGCTGCTCAGTTTCGTCCTCAAACACGTGAACATTTCACTATG
TCTCCGGGTACAAATGACGGAGCTGCGTTACGTTTAGGCGCTGCTATTAATGCCCGGCAAGGTGCGGATCTCCCTTCTAA
TTTCTTCTGGGCGCCAGTTTCTGTACTTCGTCATCCTGATGGTCGTGAGGAACGATTCCCCCATCTTGTGACTGATCGCG
CCAAGCCGGGTGTCATCGCGGTTAACCAGCAAGCAGAGCGCTTTGTTAATGAATCTAACTCTTACCATCACTTTGCCAGT
GCCATGCAAAGCCAGGCGGAAAATGCCCCTTGTTATTTGCTCTGCGATGCCCTGGCGATGAAACGCTATGGCCTGGGGTT
GGCACGTCCGTCGCCTGTCAATAATGACGCACTGGTACGGGCAGGATATTTGTACAAAGCACAGAGTCTGAACGAATTGG
CGCAGCTTCTGGGGCTTGATGCTGCAAAACTGGAAGCCACCGTAGCGCGATACAACCATGACGCCAGCAATGGTAGAGAT
GAGCAATTTCGGAAAGGCGCTAACAGTTACAACCAGGCAATGGGTGATCCTGCCCACCAGCCAAATGCCTGTAATGCGCC
TCTGCTTGAGGCTCCGTTTTACGCTATCACCCTTTATACCGGCGATTTGGGTACATCCAGAGGACTGGTCACCACACCTG
ATGCTCAGGTGCTCAACCAAAGTGGTTGCCCGATAAAGGGCCTCTACGCGGTGGGAAACGATATGGATTCCATTATGGCG
GGAACGTACCCCGGCCCAGGTATCACACTCGGTCCTGCACTGACCTTTGGTTATCTCGCCGCCAGCCACATGGCACAACA
ACAGACACCATAA

Upstream 100 bases:

>100_bases
CGCCAGCGTTACTGGAAACATTAACTTCGGCTACGCATGTTGCTGACGTAGTCACTGCCCCTGTGATGACACCACTGCTA
ACTTTTGCTCAGCAGCGTGG

Downstream 100 bases:

>100_bases
ACAGGAGATTGTCATGATTTATGAAAAACGTACCTACACCATTAACCCACTGAAAATGGCCGACTGGCTGGCCCTCTATA
AAAGCGATGCTCTGGCAGTA

Product: 3-oxosteroid 1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MCYPDRARNGTGTNETDGRIYRSDASTGRGRSMKSWDVVVIGSGAAGFAAAVSACCKGLSVLMLEKAEQFGGTSAISGGA
VWLHDTDQARELGKNDSAEAIKTYLQAIVGPENYRAEMIDAFVAQGREALAFLESEGAVKYSLRPLSPDYYPDEAGAVDV
GRALEVIEFDGRALGTRFADLRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLHSLLHCSKLLLRYGRDRLRYHRGTRLAMG
NALIARMAHVAFRKGLTLELNVNVLSLIAENGTVTGVQIEQHGQQHIVHARYGVVLAAGGFAAGELAAQFRPQTREHFTM
SPGTNDGAALRLGAAINARQGADLPSNFFWAPVSVLRHPDGREERFPHLVTDRAKPGVIAVNQQAERFVNESNSYHHFAS
AMQSQAENAPCYLLCDALAMKRYGLGLARPSPVNNDALVRAGYLYKAQSLNELAQLLGLDAAKLEATVARYNHDASNGRD
EQFRKGANSYNQAMGDPAHQPNACNAPLLEAPFYAITLYTGDLGTSRGLVTTPDAQVLNQSGCPIKGLYAVGNDMDSIMA
GTYPGPGITLGPALTFGYLAASHMAQQQTP

Sequences:

>Translated_590_residues
MCYPDRARNGTGTNETDGRIYRSDASTGRGRSMKSWDVVVIGSGAAGFAAAVSACCKGLSVLMLEKAEQFGGTSAISGGA
VWLHDTDQARELGKNDSAEAIKTYLQAIVGPENYRAEMIDAFVAQGREALAFLESEGAVKYSLRPLSPDYYPDEAGAVDV
GRALEVIEFDGRALGTRFADLRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLHSLLHCSKLLLRYGRDRLRYHRGTRLAMG
NALIARMAHVAFRKGLTLELNVNVLSLIAENGTVTGVQIEQHGQQHIVHARYGVVLAAGGFAAGELAAQFRPQTREHFTM
SPGTNDGAALRLGAAINARQGADLPSNFFWAPVSVLRHPDGREERFPHLVTDRAKPGVIAVNQQAERFVNESNSYHHFAS
AMQSQAENAPCYLLCDALAMKRYGLGLARPSPVNNDALVRAGYLYKAQSLNELAQLLGLDAAKLEATVARYNHDASNGRD
EQFRKGANSYNQAMGDPAHQPNACNAPLLEAPFYAITLYTGDLGTSRGLVTTPDAQVLNQSGCPIKGLYAVGNDMDSIMA
GTYPGPGITLGPALTFGYLAASHMAQQQTP
>Mature_590_residues
MCYPDRARNGTGTNETDGRIYRSDASTGRGRSMKSWDVVVIGSGAAGFAAAVSACCKGLSVLMLEKAEQFGGTSAISGGA
VWLHDTDQARELGKNDSAEAIKTYLQAIVGPENYRAEMIDAFVAQGREALAFLESEGAVKYSLRPLSPDYYPDEAGAVDV
GRALEVIEFDGRALGTRFADLRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLHSLLHCSKLLLRYGRDRLRYHRGTRLAMG
NALIARMAHVAFRKGLTLELNVNVLSLIAENGTVTGVQIEQHGQQHIVHARYGVVLAAGGFAAGELAAQFRPQTREHFTM
SPGTNDGAALRLGAAINARQGADLPSNFFWAPVSVLRHPDGREERFPHLVTDRAKPGVIAVNQQAERFVNESNSYHHFAS
AMQSQAENAPCYLLCDALAMKRYGLGLARPSPVNNDALVRAGYLYKAQSLNELAQLLGLDAAKLEATVARYNHDASNGRD
EQFRKGANSYNQAMGDPAHQPNACNAPLLEAPFYAITLYTGDLGTSRGLVTTPDAQVLNQSGCPIKGLYAVGNDMDSIMA
GTYPGPGITLGPALTFGYLAASHMAQQQTP

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 63603; Mature: 63603

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCYPDRARNGTGTNETDGRIYRSDASTGRGRSMKSWDVVVIGSGAAGFAAAVSACCKGLS
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHH
VLMLEKAEQFGGTSAISGGAVWLHDTDQARELGKNDSAEAIKTYLQAIVGPENYRAEMID
HHHHHHHHHHCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
AFVAQGREALAFLESEGAVKYSLRPLSPDYYPDEAGAVDVGRALEVIEFDGRALGTRFAD
HHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
LRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLHSLLHCSKLLLRYGRDRLRYHRGTRLAMG
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEHH
NALIARMAHVAFRKGLTLELNVNVLSLIAENGTVTGVQIEQHGQQHIVHARYGVVLAAGG
HHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEEEHHHCCCHHEEEEECCEEEEECC
FAAGELAAQFRPQTREHFTMSPGTNDGAALRLGAAINARQGADLPSNFFWAPVSVLRHPD
CHHHHHHHHCCCCCCCCEEECCCCCCCCEEEECCEECCCCCCCCCCCCCHHHHHHHCCCC
GREERFPHLVTDRAKPGVIAVNQQAERFVNESNSYHHFASAMQSQAENAPCYLLCDALAM
CCHHHCCCHHCCCCCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHH
KRYGLGLARPSPVNNDALVRAGYLYKAQSLNELAQLLGLDAAKLEATVARYNHDASNGRD
HHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
EQFRKGANSYNQAMGDPAHQPNACNAPLLEAPFYAITLYTGDLGTSRGLVTTPDAQVLNQ
HHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEECCCHHHHCC
SGCPIKGLYAVGNDMDSIMAGTYPGPGITLGPALTFGYLAASHMAQQQTP
CCCCEEEEEEECCCHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCYPDRARNGTGTNETDGRIYRSDASTGRGRSMKSWDVVVIGSGAAGFAAAVSACCKGLS
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHH
VLMLEKAEQFGGTSAISGGAVWLHDTDQARELGKNDSAEAIKTYLQAIVGPENYRAEMID
HHHHHHHHHHCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
AFVAQGREALAFLESEGAVKYSLRPLSPDYYPDEAGAVDVGRALEVIEFDGRALGTRFAD
HHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
LRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLHSLLHCSKLLLRYGRDRLRYHRGTRLAMG
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEHH
NALIARMAHVAFRKGLTLELNVNVLSLIAENGTVTGVQIEQHGQQHIVHARYGVVLAAGG
HHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEEEHHHCCCHHEEEEECCEEEEECC
FAAGELAAQFRPQTREHFTMSPGTNDGAALRLGAAINARQGADLPSNFFWAPVSVLRHPD
CHHHHHHHHCCCCCCCCEEECCCCCCCCEEEECCEECCCCCCCCCCCCCHHHHHHHCCCC
GREERFPHLVTDRAKPGVIAVNQQAERFVNESNSYHHFASAMQSQAENAPCYLLCDALAM
CCHHHCCCHHCCCCCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHH
KRYGLGLARPSPVNNDALVRAGYLYKAQSLNELAQLLGLDAAKLEATVARYNHDASNGRD
HHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
EQFRKGANSYNQAMGDPAHQPNACNAPLLEAPFYAITLYTGDLGTSRGLVTTPDAQVLNQ
HHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEECCCHHHHCC
SGCPIKGLYAVGNDMDSIMAGTYPGPGITLGPALTFGYLAASHMAQQQTP
CCCCEEEEEEECCCHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]