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The map label for this gene is mrdA [H]

Identifier: 218549789

GI number: 218549789

Start: 2533341

End: 2535242

Strand: Direct

Name: mrdA [H]

Synonym: EFER_2470

Alternate gene names: 218549789

Gene position: 2533341-2535242 (Clockwise)

Preceding gene: 218549788

Following gene: 218549790

Centisome position: 55.21

GC content: 49.16

Gene sequence:

>1902_bases
ATGAAACTACAGAACTCTTTTCGCGACTATACGGCAGAGTCCGCCCTCTTTGTACGTCGGGCGCTGGTCGCTTTTTTAGG
CATATTGCTGCTGACTGGCGTGCTGATTGCCAACTTATATAATCTGCAAATCGTCCGTTTTACTGACTATCAAACTCGTT
CCAACGAAAACCGTATAAAGCTGGTGCCCATTGCCCCCAGTCGCGGCATTATCTACGACCGCAATGGCATTCCTCTCGCA
CTCAACCGCACCATTTACCAGATTGAAATGATGCCGGAGAAAGTCGAGAACGTGCAGCAAACGCTGGATGCCTTGCGTAG
TGTCGTTGATCTGACTGATGACGATATCGCCGCATTTAGAAAAGAGCGTGCTCGTTCCCACCGCTTTACATCAATTCCGG
TTAAAACCAACCTGACCGAAGTTCAGGTGGCGAGATTTGCGGTGAATCAGTACCGTTTTCCTGGGGTGGAAGTTAAAGGC
TATAAACGCCGCTACTATCCCTATGGTTCGGCGTTAACCCATGTCATCGGTTATGTGGCAAAAATTAACGATAAAGACGT
CGAACGGTTAAATAATGACAACAAACTGGCTAATTATGCCGCGACACACGATATCGGCAAGCTCGGCATTGAACGTTATT
ACGAAGACGTATTACATGGTCAGACAGGTTATGAAGAGGTCGAAGTTAATAACCGAGGCCGGGTAATTCGTCAGCTTAAA
GAAGTACCGCCACAGGCTGGTCATGATATTTACCTGACGCTTGATCTGAAACTGCAACAGTATATTGAGACGTTACTGGC
AGGAAGCCGTGCTGCGGTGGTCGTTACGGATCCCCGCACCGGTGGGGTTCTGGCTCTGGTCTCAACACCGAGCTACGACC
CTAACCTGTTTGTCGATGGTATCTCCAGCAAAGAGTATTCCGCTTTGTTGAACGATCCTAATACCCCGTTGGTTAACCGT
GCCACGCAAGGCGTTTATCCACCAGCGTCTACGGTTAAACCGTATGTTGCTGTTTCTGCCTTAAGTGCAGGGGTAATCAC
CCGCAACACCAGTTTGTTCGACCCAGGCTGGTGGCAGCTACCAGGTTCTGAAAAACGCTACCGCGACTGGAAAAAATGGG
GGCATGGTCATCTTAACGTCACCAAATCACTGGAAGAGTCAGCGGATACCTTCTTCTACCAGGTCGCCTACGACATGGGT
ATTGACCGCCTTTCCGAATGGATGACCAAATTTGGTTACGGTGAGTATACAGGTATCGACCTTGCAGAAGAGCGTTCCGG
TAATATGCCGACGCGCGAATGGAAACAAAAACGCTTTAAAAAACCGTGGTATCAGGGCGATACCATTCCTGTAGGCATCG
GTCAGGGTTACTGGACAGCAACACCTATTCAGATGAGTAAGGCGCTGATGATCCTGATTAATGATGGCATTGTGAAGGTT
CCACACCTGTTAATGAGCACTGCTGAAAATGGTAAAAAAGTGCCCTGGGTGCAACCACATGAACCACCAGTGGGTGATAT
TCACTCTGGTTACTGGGAACTGGCGAAAGACGGTATGTATGGTGTGGCTAACCGTGCCAATGGCACAGCACATAAATACT
TTGCCAGTGCGCCATATAAAATTGCCGCAAAATCCGGTACGGCCCAGGTCTTTGGCCTGAAAGCAAATGAAACCTATAAC
GCACATAAAATTGCTGAGCGACTGCGCGACCATAAATTGATGACCGCATTCGCACCGTACAATAACCCACAAGTCGCGGT
TGCCATGATTCTGGAAAACGGCGGTGCTGGTCCGGCGGTTGGTACACTAATGCGACAAATTCTTGATCACATTATGCTCG
GTGATAACAACACCGTTCTGCCTGCGGAGAATCCGGCAGTGGCAGCGGCGGAGGATCAATGA

Upstream 100 bases:

>100_bases
TGTGTTGGTTGCAGAGAGTCTGTATCGGGCGTGGAGCATCACCACTAATCACCCTTATCACCGAGAGTGATCATAGAGCC
TTGAGTAGAAAACGCTGCGG

Downstream 100 bases:

>100_bases
CCATGACGGATAATCCGAATAAAAAAACATTCTGGGATAAAGTCCATCTTGATCCCACAATGTTGCTCATTTTACTGGCC
TTATTGGTATACAGCGCACT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLA
LNRTIYQIEMMPEKVENVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
YKRRYYPYGSALTHVIGYVAKINDKDVERLNNDNKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKEYSALLNDPNTPLVNR
ATQGVYPPASTVKPYVAVSALSAGVITRNTSLFDPGWWQLPGSEKRYRDWKKWGHGHLNVTKSLEESADTFFYQVAYDMG
IDRLSEWMTKFGYGEYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
PHLLMSTAENGKKVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRANGTAHKYFASAPYKIAAKSGTAQVFGLKANETYN
AHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTVLPAENPAVAAAEDQ

Sequences:

>Translated_633_residues
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLA
LNRTIYQIEMMPEKVENVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
YKRRYYPYGSALTHVIGYVAKINDKDVERLNNDNKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKEYSALLNDPNTPLVNR
ATQGVYPPASTVKPYVAVSALSAGVITRNTSLFDPGWWQLPGSEKRYRDWKKWGHGHLNVTKSLEESADTFFYQVAYDMG
IDRLSEWMTKFGYGEYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
PHLLMSTAENGKKVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRANGTAHKYFASAPYKIAAKSGTAQVFGLKANETYN
AHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTVLPAENPAVAAAEDQ
>Mature_633_residues
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLA
LNRTIYQIEMMPEKVENVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
YKRRYYPYGSALTHVIGYVAKINDKDVERLNNDNKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKEYSALLNDPNTPLVNR
ATQGVYPPASTVKPYVAVSALSAGVITRNTSLFDPGWWQLPGSEKRYRDWKKWGHGHLNVTKSLEESADTFFYQVAYDMG
IDRLSEWMTKFGYGEYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
PHLLMSTAENGKKVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRANGTAHKYFASAPYKIAAKSGTAQVFGLKANETYN
AHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTVLPAENPAVAAAEDQ

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=633, Percent_Identity=97.7883096366509, Blast_Score=1278, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=636, Percent_Identity=25.1572327044025, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70850; Mature: 70850

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEE
LVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVENVQQTLDALRSVVDLTDDDIAAFR
EEEECCCCCEEECCCCCCEEECCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVA
HHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCCHHHHHHHHHHHH
KINDKDVERLNNDNKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
HCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDG
HCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEC
ISSKEYSALLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVITRNTSLFDPGWWQL
CCCCHHHHHHCCCCCCHHHHCCCCCCCCHHCCCHHHHHHHHHCCEEECCCCCCCCCCEEC
PGSEKRYRDWKKWGHGHLNVTKSLEESADTFFYQVAYDMGIDRLSEWMTKFGYGEYTGID
CCCCHHHHHHHHHCCCCEEEHHHHHHHHHHEEEEHHHHHCHHHHHHHHHHCCCCCCCCCC
LAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
CHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEECCHHHHCCEEEEEEECCCHHH
PHLLMSTAENGKKVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRANGTAHKYFASAPYK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCCCHHHEECCCCEE
IAAKSGTAQVFGLKANETYNAHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAV
EEECCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCHHH
GTLMRQILDHIMLGDNNTVLPAENPAVAAAEDQ
HHHHHHHHHHHEECCCCEECCCCCCCEECCCCC
>Mature Secondary Structure
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEE
LVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVENVQQTLDALRSVVDLTDDDIAAFR
EEEECCCCCEEECCCCCCEEECCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVA
HHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCCHHHHHHHHHHHH
KINDKDVERLNNDNKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
HCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDG
HCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEC
ISSKEYSALLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVITRNTSLFDPGWWQL
CCCCHHHHHHCCCCCCHHHHCCCCCCCCHHCCCHHHHHHHHHCCEEECCCCCCCCCCEEC
PGSEKRYRDWKKWGHGHLNVTKSLEESADTFFYQVAYDMGIDRLSEWMTKFGYGEYTGID
CCCCHHHHHHHHHCCCCEEEHHHHHHHHHHEEEEHHHHHCHHHHHHHHHHCCCCCCCCCC
LAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
CHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEECCHHHHCCEEEEEEECCCHHH
PHLLMSTAENGKKVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRANGTAHKYFASAPYK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCCCHHHEECCCCEE
IAAKSGTAQVFGLKANETYNAHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAV
EEECCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCHHH
GTLMRQILDHIMLGDNNTVLPAENPAVAAAEDQ
HHHHHHHHHHHEECCCCEECCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]