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The map label for this gene is mrdB [H]

Identifier: 218549790

GI number: 218549790

Start: 2535245

End: 2536357

Strand: Direct

Name: mrdB [H]

Synonym: EFER_2471

Alternate gene names: 218549790

Gene position: 2535245-2536357 (Clockwise)

Preceding gene: 218549789

Following gene: 218549791

Centisome position: 55.25

GC content: 48.07

Gene sequence:

>1113_bases
ATGACGGATAATCCGAATAAAAAAACATTCTGGGATAAAGTCCATCTTGATCCCACAATGTTGCTCATTTTACTGGCCTT
ATTGGTATACAGCGCACTGGTTATCTGGAGCGCCAGCGGCCAGGATATCGGCATGATGGAACGTAAAATCGGCCAGATTG
CGATCGGGCTGGTGATTATGGTCGTGATGGCACAAATTCCACCGCGCGTCTATGAAGGCTGGGCCCCCTATCTCTATATC
ATCTGTATTATTTTGCTGGTGGCAGTAGATGCCTTTGGCGCAATTTCTAAAGGTGCCCAGCGCTGGCTGGATCTTGGTAT
CGTTCGTTTTCAGCCATCGGAGATTGCTAAAATTGCTGTACCGTTGATGGTAGCGCGTTTTATCAACCGTGATGTCTGTC
CTCCCTCCTTGAAAAATACAGCTATCGCGCTGGTACTTATTTTTATGCCAACGTTGCTGGTAGCTGCGCAACCGGACCTG
GGAACGTCAATCCTCGTCGCCCTTTCTGGTCTGTTTGTGCTTTTCCTTTCCGGCCTGAGTTGGCGGTTGATTGGTGTTGC
TGTCGTGCTGGTAGCGGCATTTATTCCGATTTTGTGGTTCTTCCTGATGCATGATTATCAGCGACAGCGTGTAATGATGC
TGCTCGACCCGGAATCCGATCCATTGGGTGCTGGTTATCACATTATTCAGTCTAAAATTGCTATTGGCTCAGGCGGATTA
CGCGGGAAGGGCTGGCTGCACGGCACTCAGTCACAGCTTGAATTTTTACCCGAACGCCACACTGACTTTATCTTCGCGGT
ACTGGCTGAAGAGTTAGGATTAGTGGGGATTTTAATTCTGCTTGCGCTCTATATTCTACTGATTATGCGCGGATTATGGA
TTGCTGCCAGAGCACAGACTACTTTTGGTCGTGTAATGGCAGGTGGTTTAATGTTGATATTGTTCGTTTATGTCTTCGTA
AATATTGGTATGGTGAGCGGTATTCTGCCAGTTGTTGGCGTACCGCTACCGCTGGTCAGTTATGGAGGCTCAGCACTCAT
CGTGCTGATGGCCGGGTTCGGGATTGTTATGTCGATCCATACCCACCGAAAAATGTTGTCGAAAAGCGTATAA

Upstream 100 bases:

>100_bases
CACTAATGCGACAAATTCTTGATCACATTATGCTCGGTGATAACAACACCGTTCTGCCTGCGGAGAATCCGGCAGTGGCA
GCGGCGGAGGATCAATGACC

Downstream 100 bases:

>100_bases
GGGGTGCGCAATGCGTAAGGAGTGGCTCGGGATATGCATCGCGGCAGGAATGCTCGCGGCGTGTACAAACGATGATGGTC
AGCAACAGACGACAGTACCG

Product: cell wall shape-determining protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAIGLVIMVVMAQIPPRVYEGWAPYLYI
ICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV

Sequences:

>Translated_370_residues
MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAIGLVIMVVMAQIPPRVYEGWAPYLYI
ICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV
>Mature_369_residues
TDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAIGLVIMVVMAQIPPRVYEGWAPYLYII
CIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLG
TSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGLR
GKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVN
IGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV

Specific function: This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation [H]

COG id: COG0772

COG function: function code D; Bacterial cell division membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ftsW/rodA/spoVE family [H]

Homologues:

Organism=Escherichia coli, GI1786853, Length=370, Percent_Identity=99.4594594594595, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1786277, Length=317, Percent_Identity=31.8611987381703, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001182
- InterPro:   IPR018365
- InterPro:   IPR011923 [H]

Pfam domain/function: PF01098 FTSW_RODA_SPOVE [H]

EC number: NA

Molecular weight: Translated: 40473; Mature: 40342

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00428 FTSW_RODA_SPOVE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAIGLVIM
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAV
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLGTSILVALSGLFVLFLSGLS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKSV
HHHHHHHCCC
>Mature Secondary Structure 
TDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAIGLVIM
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAV
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLGTSILVALSGLFVLFLSGLS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKSV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]