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The map label for this gene is fliM [H]

Identifier: 218549270

GI number: 218549270

Start: 1982572

End: 1983576

Strand: Direct

Name: fliM [H]

Synonym: EFER_1930

Alternate gene names: 218549270

Gene position: 1982572-1983576 (Clockwise)

Preceding gene: 218549269

Following gene: 218549271

Centisome position: 43.21

GC content: 50.05

Gene sequence:

>1005_bases
ATGGGTAATAGTATTCTTTCTCAGGCTGAAATCGATGCGCTGCTCAACGGAGACAGCGAGACCAAAGACGAACCTATAAG
CGGTATCACTAATGAGAGTGATATTCGCCCGTATGATCCCAATACCCAGCGGCGAGTTGTGCGTGAGCGCTTGCAGGCAC
TGGAAATCATTAACGAGCGTTTTGCCCGTCATTTCCGTATGGGATTGTTCAACCTGCTACGCCGTAGCCCGGATATTACC
GTCGGTGCGATTCGTATTCAGCCATATCACGAATTTGCCCGCAATTTGCCGGTGCCCACTAACCTTAACCTGATCCACCT
TAAACCGTTACGTGGTACAGGGCTGGTGGTGTTCTCCCCTGCTTTGGTGTTTATCGCTGTCGATAACCTGTTTGGTGGTG
ACGGACGTTTCCCAACGAAGGTTGAAGGCCGGGAGTTTACTCACACCGAACAGCGTGTCATTAACCGCATGTTGAAGCTG
GCCCTGGAAGGTTACCGCGACGCATGGAAAGCCATTAACCCGCTGGAAGTGGAGTATGTGCGTTCGGAAATGCAGGTGAA
GTTCACCAATATCACCACCTCACCAAACGATATTGTGGTTAACACGCCATTTCATGTGGAGATTGGCAACCTGACTGGTG
AATTTAATATCTGCCTACCTTTCAGCATGATTGAGCCGTTGCGGGAGCTGCTGGTTAACCCGCCGTTGGAAAACTCGCGT
CATGAAGATCAGAACTGGCGCAATAACCTGGTACGCCAGGTGCAGCACTCGCAACTGGAACTGGTGGCGAACTTTGCCGA
AATTTCGCTGCGACTTTCGCAGATATTGAAGCTAAAACCTGGCGACGTTCTGCCAATAGAAAAACCTGACCGCATAATTG
CCCATGTCGATGGCGTTCCGGTGCTGACCAGTCAGTACGGTACTCTCAACGGCCAATATGCGTTACGAATTGAACATTTG
ATTAACCCGATTTTGAATTCTCTGAACGAGGAACAGCCCAAATGA

Upstream 100 bases:

>100_bases
AAGGCAATCAAAGAGACTCTGGCCGTTCCCCTTGTTGCCGGGCAACCTAAGCAGGACGTTACCGACGTTTTGTATACAGC
CTTTATTCTGCGGTAGAGAC

Downstream 100 bases:

>100_bases
GTGACATGAATAATCCGGCCGATGAGAACAACGGCGCACTGGACGATCTGTGGGCTGAGGCATTAAGCGAGCAACAAACA
ACCAGTAGCAAAAGCGCTAC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGNSILSQAEIDALLNGDSETKDEPISGITNESDIRPYDPNTQRRVVRERLQALEIINERFARHFRMGLFNLLRRSPDIT
VGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPALVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
ALEGYRDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HEDQNWRNNLVRQVQHSQLELVANFAEISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHL
INPILNSLNEEQPK

Sequences:

>Translated_334_residues
MGNSILSQAEIDALLNGDSETKDEPISGITNESDIRPYDPNTQRRVVRERLQALEIINERFARHFRMGLFNLLRRSPDIT
VGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPALVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
ALEGYRDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HEDQNWRNNLVRQVQHSQLELVANFAEISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHL
INPILNSLNEEQPK
>Mature_333_residues
GNSILSQAEIDALLNGDSETKDEPISGITNESDIRPYDPNTQRRVVRERLQALEIINERFARHFRMGLFNLLRRSPDITV
GAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPALVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLA
LEGYRDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSRH
EDQNWRNNLVRQVQHSQLELVANFAEISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLI
NPILNSLNEEQPK

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=334, Percent_Identity=95.8083832335329, Blast_Score=653, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 38037; Mature: 37906

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSILSQAEIDALLNGDSETKDEPISGITNESDIRPYDPNTQRRVVRERLQALEIINER
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSP
HHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEECCCCCCEEEECC
ALVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYRDAWKAINPLEVEYV
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HHCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCC
HEDQNWRNNLVRQVQHSQLELVANFAEISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
EEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GNSILSQAEIDALLNGDSETKDEPISGITNESDIRPYDPNTQRRVVRERLQALEIINER
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSP
HHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEECCCCCCEEEECC
ALVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYRDAWKAINPLEVEYV
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HHCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCC
HEDQNWRNNLVRQVQHSQLELVANFAEISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
EEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]