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The map label for this gene is putP [H]

Identifier: 218549252

GI number: 218549252

Start: 1962958

End: 1964466

Strand: Reverse

Name: putP [H]

Synonym: EFER_1912

Alternate gene names: 218549252

Gene position: 1964466-1962958 (Counterclockwise)

Preceding gene: 218549254

Following gene: 218549251

Centisome position: 42.81

GC content: 52.95

Gene sequence:

>1509_bases
ATGGCTATTAGCACACCGATGTTGGTGACATTCTGTGTCTATATTTTTGGCATGATATTGATTGGGTTCATCGCCTGGCG
ATCAACCAAAAACTTCGACGACTATATTCTTGGCGGTCGCAGCCTTGGGCCATTCGTGACGGCATTATCGGCAGGTGCGT
CGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCGGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATT
GGCCTGACATTAGGCGCATGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGC
CTTAACATTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGTATTTTGCGTATTATCTCCGCGCTGGTGATTT
TGCTGTTCTTCACCATTTATTGCGCTTCGGGCATCGTGGCAGGCGCGCGTCTGTTTGAAAGTACCTTTGGCATGAGCTAC
GAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGA
CACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTGATCATTAGCGTCGGTGGGTTTGGCG
ACTCGCTGGAAGTGATCAAACAAAAGAGCATTGAAAACGTGGATATGCTCAAAGGATTGAATTTTGTCGCCATTATCTCG
CTGATGGGCTGGGGGCTGGGTTACTTCGGTCAGCCGCATATTCTGGCGCGTTTTATGGCGGCGGATTCTCACTATAGTAT
TGTCCATGCGCGTCGTATCAGCATGACCTGGATGATCCTCTGTCTGGCAGGCGCGGTGGCGGTCGGTTTCTTCGGCATTG
CATACTTTAACAATAATCCAGCGGTTGCCGGTGCGGTAAACCAGAACTCTGAGCGCGTGTTTATCGAACTGGCACAAATA
TTGTTTAACCCGTGGATTGCCGGGATTCTGTTGTCGGCGATTCTGGCGGCGGTGATGTCGACGTTAAGTTGCCAGCTGCT
GGTGTGCTCCAGTGCGATTACCGAAGATCTATACAAAGCATTTCTGCGTAAACATGCCAGCCAGAAAGAACTGGTGTGGG
TAGGGCGTGTGATGGTGTTGGTGGTGGCGCTGGTGGCAATTGCGCTGGCGGCGAATCCGGAAAACCGTGTGCTGGGCTTA
GTAAGCTACGCGTGGGCAGGCTTCGGTGCGGCGTTTGGTCCGGTGGTGCTGTTCTCAGTGATGTGGTCACGCATGACGCG
TAACGGTGCACTGGCGGGGATGATCATTGGCGCGCTGACGGTTATTGTCTGGAAACAGTTCGGCTGGCTGGGTCTGTACG
AAATTATTCCGGGCTTTATCTTCGGTAGCATTGGGATTGTGGTGTTTAGTTTACTGGGTAAAGCACCGTCAGCGGCAATG
CAAAAACGCTTTGCTGACGCCGATGCGCACTATCATTCAGCGCCGCCGTCACGGTTGCAGGAAGAGTAA

Upstream 100 bases:

>100_bases
ACGGTCGCCCTGGGAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAA
TAATTTTTTGGAGACTTTAG

Downstream 100 bases:

>100_bases
GGGAAGGCGTTTTCCCGAAAATCCTACGCCATAATCAGAAGCAGGCAGAGGGCAATCAGTGATTGCCCTCTGCGTTTTTC
CTCAAAATATTTTTACATTT

Product: proline:sodium symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAI
GLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHYSIVHARRISMTWMILCLAGAVAVGFFGIAYFNNNPAVAGAVNQNSERVFIELAQI
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
QKRFADADAHYHSAPPSRLQEE

Sequences:

>Translated_502_residues
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAI
GLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHYSIVHARRISMTWMILCLAGAVAVGFFGIAYFNNNPAVAGAVNQNSERVFIELAQI
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
QKRFADADAHYHSAPPSRLQEE
>Mature_501_residues
AISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIG
LTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYE
TALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIISL
MGWGLGYFGQPHILARFMAADSHYSIVHARRISMTWMILCLAGAVAVGFFGIAYFNNNPAVAGAVNQNSERVFIELAQIL
FNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLV
SYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQ
KRFADADAHYHSAPPSRLQEE

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=490, Percent_Identity=45.9183673469388, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI110835708, Length=487, Percent_Identity=23.6139630390144, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4507035, Length=374, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1787251, Length=502, Percent_Identity=98.605577689243, Blast_Score=992, Evalue=0.0,
Organism=Escherichia coli, GI1790503, Length=451, Percent_Identity=26.8292682926829, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87082237, Length=460, Percent_Identity=27.3913043478261, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115533094, Length=425, Percent_Identity=26.1176470588235, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI221459584, Length=389, Percent_Identity=22.879177377892, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645928, Length=357, Percent_Identity=25.7703081232493, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221459588, Length=427, Percent_Identity=24.1217798594848, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24651741, Length=371, Percent_Identity=22.911051212938, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221459582, Length=401, Percent_Identity=24.6882793017456, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24650192, Length=402, Percent_Identity=21.6417910447761, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221459586, Length=376, Percent_Identity=22.3404255319149, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24651739, Length=385, Percent_Identity=22.0779220779221, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24640370, Length=356, Percent_Identity=24.438202247191, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 54377; Mature: 54246

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCEE
LMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFED
EECCCHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEEEEEEECCCEEECCCHHCCCCCC
KSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
HEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHH
LMGWGLGYFGQPHILARFMAADSHYSIVHARRISMTWMILCLAGAVAVGFFGIAYFNNNP
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
AVAGAVNQNSERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHH
MWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QKRFADADAHYHSAPPSRLQEE
HHHHCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
AISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCEE
LMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFED
EECCCHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEEEEEEECCCEEECCCHHCCCCCC
KSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
HEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHH
LMGWGLGYFGQPHILARFMAADSHYSIVHARRISMTWMILCLAGAVAVGFFGIAYFNNNP
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
AVAGAVNQNSERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHH
MWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QKRFADADAHYHSAPPSRLQEE
HHHHCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]