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The map label for this gene is msbB [H]

Identifier: 218549254

GI number: 218549254

Start: 1968944

End: 1969885

Strand: Reverse

Name: msbB [H]

Synonym: EFER_1914

Alternate gene names: 218549254

Gene position: 1969885-1968944 (Counterclockwise)

Preceding gene: 218549255

Following gene: 218549252

Centisome position: 42.93

GC content: 42.04

Gene sequence:

>942_bases
ATGAGAAAAAAACAGTTTGAGTTTATTCCTGAATTTAAGAAATCTTTCTTACTTCCGCGTTATTGGAGTACATGGTGTGG
CCTGATAATCATAGTAATCTTTGCGTTCATTCCTCCATCAATTCGTGATCCTGCTCTGGGTAAACTTGGGCGTGTGGTCG
GACGGTTGGGGAAAAATGCTCGTCGGCGGGCATTGATTAATCTGGCTCTTTGCTTCCCGGAACGTAGTAAGAAAGAATGC
GAGAGCATTGTTGACGCTATGTTTGCCACAGCATTGCAATCTATCGTGATGCTGGCAGAGTTAGTTATTCGCGGGCCGGA
AAAAATCCTTCCGCGCGTACACTGGAAAAATAAAAATATCCTGGAAGAAATAAAATATAACAACGACAAAGTAATTTTTC
TTGTTCCGCATGGTTGGTGTGTAGATCTTCCGGCGATGTTGATGGCTTTTCAGAACCAAAAAATGGCAGCTATGTTCCAT
AACCAGCGTAATGCGGTGTTGGATTATACCTGGAATGTTGTACGCCGTATTTTCGGTGGAAGGCTACATGCCCGAAATGA
CGGGATAAAACCATTTATTCAGTCTGTACGTCAGGGATATTGGGGATATTACTTACCTGATCAGGATCATGGTCCAGAGC
ATAGTGAGTTTGTTGATTTTTTCGCAACTTATAAAGCGACATTACCTGTACCAGGACGGTTGGCTAAAATAACTCAGGCG
AAGATTATCCCCCTCTTTCCTACCTACGATGCAAAAAAACATATCCTGACCATTGAAATTCACACGCCAATGGTCGATTT
ATCGACAGCAGATGATAAAACGATAGCCCGACGGATAAATGAAGAGATAGAAAGTTTTATCAGACCAAATCCAGAACAAT
ATACCTGGATGTTGAAATTATTGAAAACACGCAAGCCTAATGAGGTTGACCCGTATCCATGA

Upstream 100 bases:

>100_bases
GAGATTACGGCCCGCCTGAATTAGTCAGAGTCTGGCAGGTAACACTTTTGGCATCTTGCCTCGCCAGTAATGTAATAAAA
CAAGCCAGCTTATATAAAAA

Downstream 100 bases:

>100_bases
CATTACCATGCGTTGGTTAGCAGCTGATGAAATGAGCGTTGCTACCGATCATGCCGGATGAGACGCGTTGCACGTCGCAT
CCGGCAACCTGTTTAACCGA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRKKQFEFIPEFKKSFLLPRYWSTWCGLIIIVIFAFIPPSIRDPALGKLGRVVGRLGKNARRRALINLALCFPERSKKEC
ESIVDAMFATALQSIVMLAELVIRGPEKILPRVHWKNKNILEEIKYNNDKVIFLVPHGWCVDLPAMLMAFQNQKMAAMFH
NQRNAVLDYTWNVVRRIFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPVPGRLAKITQA
KIIPLFPTYDAKKHILTIEIHTPMVDLSTADDKTIARRINEEIESFIRPNPEQYTWMLKLLKTRKPNEVDPYP

Sequences:

>Translated_313_residues
MRKKQFEFIPEFKKSFLLPRYWSTWCGLIIIVIFAFIPPSIRDPALGKLGRVVGRLGKNARRRALINLALCFPERSKKEC
ESIVDAMFATALQSIVMLAELVIRGPEKILPRVHWKNKNILEEIKYNNDKVIFLVPHGWCVDLPAMLMAFQNQKMAAMFH
NQRNAVLDYTWNVVRRIFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPVPGRLAKITQA
KIIPLFPTYDAKKHILTIEIHTPMVDLSTADDKTIARRINEEIESFIRPNPEQYTWMLKLLKTRKPNEVDPYP
>Mature_313_residues
MRKKQFEFIPEFKKSFLLPRYWSTWCGLIIIVIFAFIPPSIRDPALGKLGRVVGRLGKNARRRALINLALCFPERSKKEC
ESIVDAMFATALQSIVMLAELVIRGPEKILPRVHWKNKNILEEIKYNNDKVIFLVPHGWCVDLPAMLMAFQNQKMAAMFH
NQRNAVLDYTWNVVRRIFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPVPGRLAKITQA
KIIPLFPTYDAKKHILTIEIHTPMVDLSTADDKTIARRINEEIESFIRPNPEQYTWMLKLLKTRKPNEVDPYP

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=311, Percent_Identity=70.096463022508, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=28.4313725490196, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=25.4071661237785, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36393; Mature: 36393

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKQFEFIPEFKKSFLLPRYWSTWCGLIIIVIFAFIPPSIRDPALGKLGRVVGRLGKNA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHH
RRRALINLALCFPERSKKECESIVDAMFATALQSIVMLAELVIRGPEKILPRVHWKNKNI
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
LEEIKYNNDKVIFLVPHGWCVDLPAMLMAFQNQKMAAMFHNQRNAVLDYTWNVVRRIFGG
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCC
RLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPVPGRLAKITQA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHC
KIIPLFPTYDAKKHILTIEIHTPMVDLSTADDKTIARRINEEIESFIRPNPEQYTWMLKL
EEEEECCCCCCCCEEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LKTRKPNEVDPYP
HHCCCCCCCCCCC
>Mature Secondary Structure
MRKKQFEFIPEFKKSFLLPRYWSTWCGLIIIVIFAFIPPSIRDPALGKLGRVVGRLGKNA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHH
RRRALINLALCFPERSKKECESIVDAMFATALQSIVMLAELVIRGPEKILPRVHWKNKNI
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
LEEIKYNNDKVIFLVPHGWCVDLPAMLMAFQNQKMAAMFHNQRNAVLDYTWNVVRRIFGG
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCC
RLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPVPGRLAKITQA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHC
KIIPLFPTYDAKKHILTIEIHTPMVDLSTADDKTIARRINEEIESFIRPNPEQYTWMLKL
EEEEECCCCCCCCEEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LKTRKPNEVDPYP
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]