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The map label for this gene is lpxL [H]

Identifier: 218549216

GI number: 218549216

Start: 1921462

End: 1922382

Strand: Direct

Name: lpxL [H]

Synonym: EFER_1874

Alternate gene names: 218549216

Gene position: 1921462-1922382 (Clockwise)

Preceding gene: 218549214

Following gene: 218549217

Centisome position: 41.87

GC content: 51.57

Gene sequence:

>921_bases
ATGACGAATATACCCAAGTTCTCCTCAGCTCTACTTCATCCACGTTACTGGTTAACCTGGTTGGGTATTGGCGCACTTTG
GTTGGTCGTGCAGTTACCCTACCCGATTATCTATCGTCTGGGCTGCGCGATGGGGCGTCTGGCGTTACATGTTATGAAAC
GACGCGCCCAAATTGCTTATCGCAATCTGGAGCTTTGCTTTCCAGAAATGAGTGAGCAAGAGCGCCATGAGATGGTCGTG
AAAAATTTCGAATCCGTAGGCATGGGGCTGATGGAAACCGGAATGGCATGGTTCTGGCCAGACCGTCGTATTGCGCGTTG
GACAGATGTGATTGGTATGGAGCATATCCGTGAAGTTCAGGCGCAAAAGCGCGGTATCTTACTGGTCGGCATTCATTTTT
TAACACTTGAACTTGGTGCTCGCCAGTTTGGTATGCAGGAACCAGGGATTGGTGTTTATCGCCCGAATGATAATCCGCTG
ATCGACTGGCTACAAACCTGGGGTCGCTTGCGCTCCAATAAATCGATGCTTGATCGTAAGGATCTGAAAGGCATGATCAA
GGCGCTCAAAAAGGGGGAAGTCGTGTGGTATGCCCCCGATCATGATTACGGTCCACGGGCCAGTGTGTTTGTACCGCTGT
TTGCTGTAGAACAAGCCGCCACCACCTCTGGCACCTGGATGCTGGCGAAAATGTCAGGTGCCTGCCTGGTACCGTTCGTT
CCCCGCCGCAAACCTGATGGTAAGGGGTACGAACTGATTATGCTGCCACCAGAATGCTCACCACCGCTGGATAATGCCGA
AACGACCGCTGCGTGGATGAACAAAGTCGTCGAGAAATGCATCATGATGGCCCCTGAGCAATATATGTGGCTGCATCGTC
GCTTTAAAACGCGCCCGCAAGGTGTTCCTTCGCGTTATTAA

Upstream 100 bases:

>100_bases
TGGTAATTTCACCCATTCTGATAGCGTATATTTTAGTTACGGACGAAAAAGTGCAACAATACATAGAATTGCCAGTTATA
GGTTCAAACACAGGATTGAC

Downstream 100 bases:

>100_bases
TTCATTTCCTCGTAAATCGGATAAGATGCAACCCGCATCTTATCCGGCAACAACCGCTCTGCCGTAATTTAGCGATAAAA
GCTCTCCACATTGCAGCATC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTNIPKFSSALLHPRYWLTWLGIGALWLVVQLPYPIIYRLGCAMGRLALHVMKRRAQIAYRNLELCFPEMSEQERHEMVV
KNFESVGMGLMETGMAWFWPDRRIARWTDVIGMEHIREVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRASVFVPLFAVEQAATTSGTWMLAKMSGACLVPFV
PRRKPDGKGYELIMLPPECSPPLDNAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPQGVPSRY

Sequences:

>Translated_306_residues
MTNIPKFSSALLHPRYWLTWLGIGALWLVVQLPYPIIYRLGCAMGRLALHVMKRRAQIAYRNLELCFPEMSEQERHEMVV
KNFESVGMGLMETGMAWFWPDRRIARWTDVIGMEHIREVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRASVFVPLFAVEQAATTSGTWMLAKMSGACLVPFV
PRRKPDGKGYELIMLPPECSPPLDNAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPQGVPSRY
>Mature_305_residues
TNIPKFSSALLHPRYWLTWLGIGALWLVVQLPYPIIYRLGCAMGRLALHVMKRRAQIAYRNLELCFPEMSEQERHEMVVK
NFESVGMGLMETGMAWFWPDRRIARWTDVIGMEHIREVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLI
DWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRASVFVPLFAVEQAATTSGTWMLAKMSGACLVPFVP
RRKPDGKGYELIMLPPECSPPLDNAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPQGVPSRY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=92.8104575163399, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=53.8205980066445, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=32.5806451612903, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35293; Mature: 35162

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIPKFSSALLHPRYWLTWLGIGALWLVVQLPYPIIYRLGCAMGRLALHVMKRRAQIAY
CCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPEMSEQERHEMVVKNFESVGMGLMETGMAWFWPDRRIARWTDVIGMEHIREVQ
HHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
AQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRK
HHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHH
DLKGMIKALKKGEVVWYAPDHDYGPRASVFVPLFAVEQAATTSGTWMLAKMSGACLVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCEEEHHHHHHHHCCCCCCEEEEECCCCEEECCC
PRRKPDGKGYELIMLPPECSPPLDNAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPQ
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
GVPSRY
CCCCCC
>Mature Secondary Structure 
TNIPKFSSALLHPRYWLTWLGIGALWLVVQLPYPIIYRLGCAMGRLALHVMKRRAQIAY
CCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPEMSEQERHEMVVKNFESVGMGLMETGMAWFWPDRRIARWTDVIGMEHIREVQ
HHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
AQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRK
HHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHH
DLKGMIKALKKGEVVWYAPDHDYGPRASVFVPLFAVEQAATTSGTWMLAKMSGACLVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCEEEHHHHHHHHCCCCCCEEEEECCCCEEECCC
PRRKPDGKGYELIMLPPECSPPLDNAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPQ
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
GVPSRY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]