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The map label for this gene is mdtG [H]

Identifier: 218549217

GI number: 218549217

Start: 1922565

End: 1923791

Strand: Direct

Name: mdtG [H]

Synonym: EFER_1875

Alternate gene names: 218549217

Gene position: 1922565-1923791 (Clockwise)

Preceding gene: 218549216

Following gene: 218549218

Centisome position: 41.9

GC content: 50.53

Gene sequence:

>1227_bases
ATGTCTCCTTCTGAATCTGACGACGCTATAAACTGGAAACGAAATCTTATTGTTGCCTGGCTGGGCTGTTTTCTCACCGG
CGCTGCGTTCAGTCTGGTCATGCCTTTCTTACCTCTCTATGTCGAAGAACTCGGTGTCACTGGTCACAGTGCCCTGAACT
TATGGTCCGGGATCGTCTTCAGCATTACCTTCCTTTTCTCCGCGATCGCATCACCGTTTTGGGGCGGACTGGCTGACCGT
AAAGGTCGAAAAATCATGCTATTGCGCTCAGCCCTGGGAATGGGGATCGTCATGATTTTAATGGGGATGGCACAAAATAT
CTGGCAGTTTTTGATCCTGCGTGCACTGCTGGGATTGCTGGGTGGCTTTGTCCCTAATGCGAATGCGTTGATCGCAACAC
AAGTGCCCCGCAACAAAAGTGGTTGGGCGCTGGGCACACTCTCAACCGGTGGTGTAAGTGGCGCACTACTTGGTCCCTTA
GCTGGCGGTTTACTGGCTGATCACTACGGTTTACGCCCGGTGTTTTTTATAACAGCCAGCGTGCTAATGCTCTGCTTCGT
GGTAACGCTCTTGTGTATCAAAGAGAAGTTTCAGCCCGTCAGTAAGAAAGAAATGCTGCATGTGCGCGAGGTCGTGTTCT
CGCTGAAGAATCCCAGGCTGGTGTTGAGTCTTTTTGTTACCACGATGATTATTCAGATAGCTTCCGGCTCCATCGCCCCC
ATTCTTACGCTCTATGTCCGTGAACTGGCCGGTAACGTCGGCAATATCGCTTTTATCAGCGGCATGATCGCCTCTGTACC
TGGTGTTGCTGCTCTTCTTAGCGCCCCACGTCTTGGTAAACTTGGAGATCGCATCGGGCCGGATAAGATCCTGATTGCTG
CGCTGATCTTCTCTGTTCTGCTGTTGATCCCGATGTCGTTTGTTCAGACACCGTGGCAACTCGGCATCCTTCGTTTTTTA
CTGGGAGCTGCTGACGGTGCATTACTTCCCGCTGTTCAAACATTGCTGGTGTATAACTCCTCTAACCAAATTGCCGGGCG
TATTTTCAGTTATAACCAGTCATTTCGCGACATCGGTAACGTTACCGGCCCGTTAATGGGGGCCGCGATATCGGCTAACT
ATGGTTTCCGCGCAGTTTTTCTGGTCACTGCATGTGTCGTGCTGTTTAACATAATTTATTCCTGGAATAGTTTACGTCGC
CGCCGCGAACCTCAAGTATTAGGTTGA

Upstream 100 bases:

>100_bases
TCTCCACATTGCAGCATCTATATGTCAGGCGCATAATTAGTGTGCTTACCTTTTTCTCCTGTAAATACAAGTGCCCCCTG
CTTACAAACTGTGGATTACT

Downstream 100 bases:

>100_bases
TTTTTACCCATTCAGACTTGCAAAACTGGAGAATCAGCTATTCTTTTCCCTGAAACCTCATCAAATTAAAGGGAGAAACG
TGATGACCATGTACGCAACA

Product: drug efflux system protein MdtG

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSPSESDDAINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEELGVTGHSALNLWSGIVFSITFLFSAIASPFWGGLADR
KGRKIMLLRSALGMGIVMILMGMAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPL
AGGLLADHYGLRPVFFITASVLMLCFVVTLLCIKEKFQPVSKKEMLHVREVVFSLKNPRLVLSLFVTTMIIQIASGSIAP
ILTLYVRELAGNVGNIAFISGMIASVPGVAALLSAPRLGKLGDRIGPDKILIAALIFSVLLLIPMSFVQTPWQLGILRFL
LGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTACVVLFNIIYSWNSLRR
RREPQVLG

Sequences:

>Translated_408_residues
MSPSESDDAINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEELGVTGHSALNLWSGIVFSITFLFSAIASPFWGGLADR
KGRKIMLLRSALGMGIVMILMGMAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPL
AGGLLADHYGLRPVFFITASVLMLCFVVTLLCIKEKFQPVSKKEMLHVREVVFSLKNPRLVLSLFVTTMIIQIASGSIAP
ILTLYVRELAGNVGNIAFISGMIASVPGVAALLSAPRLGKLGDRIGPDKILIAALIFSVLLLIPMSFVQTPWQLGILRFL
LGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTACVVLFNIIYSWNSLRR
RREPQVLG
>Mature_407_residues
SPSESDDAINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEELGVTGHSALNLWSGIVFSITFLFSAIASPFWGGLADRK
GRKIMLLRSALGMGIVMILMGMAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPLA
GGLLADHYGLRPVFFITASVLMLCFVVTLLCIKEKFQPVSKKEMLHVREVVFSLKNPRLVLSLFVTTMIIQIASGSIAPI
LTLYVRELAGNVGNIAFISGMIASVPGVAALLSAPRLGKLGDRIGPDKILIAALIFSVLLLIPMSFVQTPWQLGILRFLL
GAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTACVVLFNIIYSWNSLRRR
REPQVLG

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=406, Percent_Identity=92.3645320197044, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI87082404, Length=304, Percent_Identity=25.3289473684211, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319871, Length=164, Percent_Identity=29.2682926829268, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43912; Mature: 43781

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSESDDAINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEELGVTGHSALNLWSGIVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SITFLFSAIASPFWGGLADRKGRKIMLLRSALGMGIVMILMGMAQNIWQFLILRALLGLL
HHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPLAGGLLADHYGLRPVFFITAS
CCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
VLMLCFVVTLLCIKEKFQPVSKKEMLHVREVVFSLKNPRLVLSLFVTTMIIQIASGSIAP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHH
ILTLYVRELAGNVGNIAFISGMIASVPGVAALLSAPRLGKLGDRIGPDKILIAALIFSVL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHH
LLIPMSFVQTPWQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGN
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHH
VTGPLMGAAISANYGFRAVFLVTACVVLFNIIYSWNSLRRRREPQVLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPSESDDAINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEELGVTGHSALNLWSGIVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SITFLFSAIASPFWGGLADRKGRKIMLLRSALGMGIVMILMGMAQNIWQFLILRALLGLL
HHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPLAGGLLADHYGLRPVFFITAS
CCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
VLMLCFVVTLLCIKEKFQPVSKKEMLHVREVVFSLKNPRLVLSLFVTTMIIQIASGSIAP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHH
ILTLYVRELAGNVGNIAFISGMIASVPGVAALLSAPRLGKLGDRIGPDKILIAALIFSVL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHH
LLIPMSFVQTPWQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGN
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHH
VTGPLMGAAISANYGFRAVFLVTACVVLFNIIYSWNSLRRRREPQVLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA