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The map label for this gene is thiK [H]

Identifier: 218549168

GI number: 218549168

Start: 1876935

End: 1877747

Strand: Reverse

Name: thiK [H]

Synonym: EFER_1822

Alternate gene names: 218549168

Gene position: 1877747-1876935 (Counterclockwise)

Preceding gene: 218549169

Following gene: 218549167

Centisome position: 40.92

GC content: 50.92

Gene sequence:

>813_bases
GTGCAGTACAGCAGCAATAATTCGCTAACGCGCGACGAGATACTGTCGCGCTATTTCCCGCAATATTATCTTGTGGAGTC
CTGCCAGTGTGGGCTTAGCGGCAGTAGTGTTGTTATTGAAAATGATGAGCAGCGTTTAGTTTTGCGCCGTCCGCATGATC
CCTCATCAGCTGAATCAATATTTCTGCGCCAGTATCATGTGCTTTCCCGTCTGCCAGAAACGCTGGCGCCAAGGCCTCGG
TTGTATCGCCCTGGATGGATGGCAGTCGATTATCTCGTGGGAGAAGTGCCTTCCTGCCTGCCAGATACCAACGAACTTGT
GTGTTTACTGTATCATCTGCATCAACAACCGTGTTTTGGCTGGCGGATATTGCTGTTACCCCTTCTGGAACAATACTGGC
AGGGAAGTGATCCTGCACGTCGCACGCCGTTGTGGTTACGTTGGCTTAAACGTTTACGTCATGTTCGTGAACCGCAACCA
TTACGATTAAGCCCATTGCATATGGATGTTCATGCCGCAAATCTGGTAATTACTGCAAAAGGACTGCGGTTGATTGACTG
GGAGTATGCTGGTGATGGTGATGTGGCGTTGGAGCTGGCTGCGGTTTGGGTTGAAAACGAACAACAGCAACGGGCACTAG
TTAACGATTATGCGCACCTTGCCAGGATAGACGCCGGGCAGTTGTGGCGTCAGGTTAGACGCTGGCAACCATGGCTACAG
ATGCTGAAAGCAGGGTGGTTTGAGTACCGCTGGCAACAGACCGGCGAAGAACACTTTATCAGGCTGGCCGATGAAACCTG
GCATCAGTTTTAA

Upstream 100 bases:

>100_bases
TATGTGCTTTACTCTAGTGCGTCTGGCAATGTCAACGCACCAACCTTACAAATGCAACTGATGCTGGTACAGACCGGCGA
GATCATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
TAGAAGAATAAGGAGAGCAGTGTGGGTCCAGTAATGTTGGATGTCGAAGGTTATGAACTGGACGCGGAAGAGAGAGAAAT
ATTGGCACATCCACTGGTTG

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MQYSSNNSLTRDEILSRYFPQYYLVESCQCGLSGSSVVIENDEQRLVLRRPHDPSSAESIFLRQYHVLSRLPETLAPRPR
LYRPGWMAVDYLVGEVPSCLPDTNELVCLLYHLHQQPCFGWRILLLPLLEQYWQGSDPARRTPLWLRWLKRLRHVREPQP
LRLSPLHMDVHAANLVITAKGLRLIDWEYAGDGDVALELAAVWVENEQQQRALVNDYAHLARIDAGQLWRQVRRWQPWLQ
MLKAGWFEYRWQQTGEEHFIRLADETWHQF

Sequences:

>Translated_270_residues
MQYSSNNSLTRDEILSRYFPQYYLVESCQCGLSGSSVVIENDEQRLVLRRPHDPSSAESIFLRQYHVLSRLPETLAPRPR
LYRPGWMAVDYLVGEVPSCLPDTNELVCLLYHLHQQPCFGWRILLLPLLEQYWQGSDPARRTPLWLRWLKRLRHVREPQP
LRLSPLHMDVHAANLVITAKGLRLIDWEYAGDGDVALELAAVWVENEQQQRALVNDYAHLARIDAGQLWRQVRRWQPWLQ
MLKAGWFEYRWQQTGEEHFIRLADETWHQF
>Mature_270_residues
MQYSSNNSLTRDEILSRYFPQYYLVESCQCGLSGSSVVIENDEQRLVLRRPHDPSSAESIFLRQYHVLSRLPETLAPRPR
LYRPGWMAVDYLVGEVPSCLPDTNELVCLLYHLHQQPCFGWRILLLPLLEQYWQGSDPARRTPLWLRWLKRLRHVREPQP
LRLSPLHMDVHAANLVITAKGLRLIDWEYAGDGDVALELAAVWVENEQQQRALVNDYAHLARIDAGQLWRQVRRWQPWLQ
MLKAGWFEYRWQQTGEEHFIRLADETWHQF

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=270, Percent_Identity=69.2592592592593, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 31994; Mature: 31994

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSSNNSLTRDEILSRYFPQYYLVESCQCGLSGSSVVIENDEQRLVLRRPHDPSSAESI
CCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCHHHHH
FLRQYHVLSRLPETLAPRPRLYRPGWMAVDYLVGEVPSCLPDTNELVCLLYHLHQQPCFG
HHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCH
WRILLLPLLEQYWQGSDPARRTPLWLRWLKRLRHVREPQPLRLSPLHMDVHAANLVITAK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCEEEEEEC
GLRLIDWEYAGDGDVALELAAVWVENEQQQRALVNDYAHLARIDAGQLWRQVRRWQPWLQ
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MLKAGWFEYRWQQTGEEHFIRLADETWHQF
HHHHHHHHHHHHCCCHHHHEEHHHHHHCCC
>Mature Secondary Structure
MQYSSNNSLTRDEILSRYFPQYYLVESCQCGLSGSSVVIENDEQRLVLRRPHDPSSAESI
CCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCHHHHH
FLRQYHVLSRLPETLAPRPRLYRPGWMAVDYLVGEVPSCLPDTNELVCLLYHLHQQPCFG
HHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCH
WRILLLPLLEQYWQGSDPARRTPLWLRWLKRLRHVREPQPLRLSPLHMDVHAANLVITAK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCEEEEEEC
GLRLIDWEYAGDGDVALELAAVWVENEQQQRALVNDYAHLARIDAGQLWRQVRRWQPWLQ
CCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MLKAGWFEYRWQQTGEEHFIRLADETWHQF
HHHHHHHHHHHHCCCHHHHEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA