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The map label for this gene is ymfC [H]

Identifier: 218549142

GI number: 218549142

Start: 1850390

End: 1851043

Strand: Direct

Name: ymfC [H]

Synonym: EFER_1795

Alternate gene names: 218549142

Gene position: 1850390-1851043 (Clockwise)

Preceding gene: 218549139

Following gene: 218549143

Centisome position: 40.32

GC content: 50.61

Gene sequence:

>654_bases
ATGCGGCAATTCATAATCTCTGAAAATACCATGCAAAAAACTTCTTTTAGAAATCACCAGGTTAAGCGATTCAGCTCGCA
ACGTTCTACCAGGCGAAAACCTGAAAACCAGCCCACGCGTGTGATCCTGTTCAATAAACCCTACGATGTTCTTCCGCAGT
TCACCGATGAAGCCGGACGCAAAACATTAAAAGAATTCATCCCGATTCAGGGGGTGTATGCGGCAGGTCGTCTCGACCGC
GACAGCGAAGGGTTACTGGTGCTGACCAATAACGGCGCTTTGCAGGCGCGTTTAACCCAGCCGGGTAAACGTACCGGGAA
AATCTATTATGTACAGGTAGAAGGTATTCCCACGGAAGACGCCCTTGAAGCGTTGCGCAATGGCGTAACCTTAAATGATG
GCCCTACCCTGCCCGCAGGTGCGGAGATGGTTGAGGAGCCGGAATGGTTATGGCCCCGTAATCCGCCAATTCGTGAACGA
AAAAGTATTCCCACCAGCTGGCTTAAGATCACCTTATATGAAGGACGTAATCGCCAGGTGCGCCGCATGACCGCCCATGT
TGGTTTCCCCACGCTGCGACTGATTCGCTATGCGATGGGTGATTACTCTTTGGATAATCTTGCCAATGGCGAATGGCGAG
ACGTGACAGATTAA

Upstream 100 bases:

>100_bases
ACGGATGCGTTAGAGCAATTTTTTGTTAATGATTTGTAATTGGCTTGTCAATACTACCCTATTGTTTGCCGTCATGAAAG
ATGCGCGTTATTAGGCTATA

Downstream 100 bases:

>100_bases
GGATGCAAAATGTTTAAACCGCACGTTACCGTTGCTTGCGTGGTGCACGCAGAAGGCAAATTTTTAGTCGTTGAAGAGAC
GATTAATGGTAAAGCGTTAT

Product: 23S rRNA pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPIQGVYAAGRLDR
DSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDVTD

Sequences:

>Translated_217_residues
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPIQGVYAAGRLDR
DSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDVTD
>Mature_217_residues
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPIQGVYAAGRLDR
DSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDVTD

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=217, Percent_Identity=97.2350230414747, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1790453, Length=184, Percent_Identity=30.9782608695652, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1787524, Length=193, Percent_Identity=33.160621761658, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1788510, Length=184, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24972; Mature: 24972

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGR
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHH
KTLKEFIPIQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTED
HHHHHHCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHH
ALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRERKSIPTSWLKITLYEGRNRQV
HHHHHHCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCHH
RRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDVTD
HHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGR
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHH
KTLKEFIPIQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTED
HHHHHHCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHH
ALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRERKSIPTSWLKITLYEGRNRQV
HHHHHHCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCHH
RRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDVTD
HHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]