Click here to switch to the map view.

The map label for this gene is frmA [H]

Identifier: 218548934

GI number: 218548934

Start: 1622329

End: 1623462

Strand: Reverse

Name: frmA [H]

Synonym: EFER_1582

Alternate gene names: 218548934

Gene position: 1623462-1622329 (Counterclockwise)

Preceding gene: 218548935

Following gene: 218548925

Centisome position: 35.38

GC content: 49.65

Gene sequence:

>1134_bases
ATGGGGAAGAGACAAATGAAATCACGTGCCGCAGTTGCATTTGGCCCTGGCCAACCACTTTCTATCGTTGAGATTGATGT
CGCACCACCAAAAAAAGGTGAAGTGCTGGTTAAAATCACCCATACCGGCGTTTGTCATACAGATGCTTTTACTTTGTCCG
GGGATGATCCAGAAGGCGTATTTCCGGCTGTGTTAGGGCATGAAGGCGGGGGAATTGTGGTTGAGGTTGGGGAAGGCGTT
ACCAGTCTTAAACCTGGCGATCATGTTATTCCGCTATATACCGCAGAGTGCGGAGAATGCAAATTCTGTAAATCAGGTAA
GACCAATCTGTGTCAGGCGGTTCGCGCTACTCAGGGAAAAGGGTTGATGCCAGATGGTACGACGCGTTTTTCTTACAATG
GTCAGCCGATTTACCACTATATGGGCACCAGTACTTTCAGTGAATATACCGTTTGCGCTGAAATCTCGTTGGCTAAAGTT
AATCCGCAAGCCCCTCTGGATAAAGTCTGTTTGCTGGGCTGCGGCGTTACCACAGGCATCGGTGCTGTACATAACACAGC
CAAAGTGAAAGCAGGCGATACCGTTGCGGTGTTTGGCCTGGGGGGGATTGGTCTGGCGGTTATCCAGGGGGCCGTGCAGG
CAAAGGCCGGGCGGATACTGGCAGTAGATACTAATCCTGAGAAGTTCAAACTGGCAGCTGAAATGGGCGCAACAGATTTC
ATTAATCCTGGTGATTACGATAAACCGATTCAGGATGTGATTGTTGAACTGACAGATGGTGGCGTCGATTTCAGTTTTGA
ATGTATTGGCAATGTGAATGTCATGCGCGCGGCGCTGGAGTGCTGCCATAAAGGATGGGGTGAGAGCATTATTATTGGCG
TGGCTGGCGCGGGTCAGGAAATCAAAACACGACCATTCCAGTTGGTGACGGGGCGTGTATGGCGTGGCTCAGCTTTTGGT
GGGGTCAAAGGTCGTACGCAACTTCCCGGCATGGTTGAAGACGCGATGGCAGGTAAAATTCAACTTGATCCATTTATCAC
GCATCGCCTGCCGCTGGAAAATATCAACGAAGCCTTTGACCTCATGCATCAAGGAAAATCGATTCGTACGGTGATCCATT
TCGGTGATAAATAA

Upstream 100 bases:

>100_bases
CTGGTTGGCGGTGAAACAACACCCGATCAACGCGCGGTGAAGATGGCTGAAATCAGCCACTTGCTACGCACTTATCTAAA
ATAAAATATCACCTTGCATA

Downstream 100 bases:

>100_bases
GAACCCGCTAGCTTATTTGATCTTCGATATCTTGCCCACCTTTGTGGGCAAGGTGTACTAAGAGTCGATACGCAGAGGAA
GCGCCGAAGTCATCCAGCCA

Product: alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MGKRQMKSRAAVAFGPGQPLSIVEIDVAPPKKGEVLVKITHTGVCHTDAFTLSGDDPEGVFPAVLGHEGGGIVVEVGEGV
TSLKPGDHVIPLYTAECGECKFCKSGKTNLCQAVRATQGKGLMPDGTTRFSYNGQPIYHYMGTSTFSEYTVCAEISLAKV
NPQAPLDKVCLLGCGVTTGIGAVHNTAKVKAGDTVAVFGLGGIGLAVIQGAVQAKAGRILAVDTNPEKFKLAAEMGATDF
INPGDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRAALECCHKGWGESIIIGVAGAGQEIKTRPFQLVTGRVWRGSAFG
GVKGRTQLPGMVEDAMAGKIQLDPFITHRLPLENINEAFDLMHQGKSIRTVIHFGDK

Sequences:

>Translated_377_residues
MGKRQMKSRAAVAFGPGQPLSIVEIDVAPPKKGEVLVKITHTGVCHTDAFTLSGDDPEGVFPAVLGHEGGGIVVEVGEGV
TSLKPGDHVIPLYTAECGECKFCKSGKTNLCQAVRATQGKGLMPDGTTRFSYNGQPIYHYMGTSTFSEYTVCAEISLAKV
NPQAPLDKVCLLGCGVTTGIGAVHNTAKVKAGDTVAVFGLGGIGLAVIQGAVQAKAGRILAVDTNPEKFKLAAEMGATDF
INPGDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRAALECCHKGWGESIIIGVAGAGQEIKTRPFQLVTGRVWRGSAFG
GVKGRTQLPGMVEDAMAGKIQLDPFITHRLPLENINEAFDLMHQGKSIRTVIHFGDK
>Mature_376_residues
GKRQMKSRAAVAFGPGQPLSIVEIDVAPPKKGEVLVKITHTGVCHTDAFTLSGDDPEGVFPAVLGHEGGGIVVEVGEGVT
SLKPGDHVIPLYTAECGECKFCKSGKTNLCQAVRATQGKGLMPDGTTRFSYNGQPIYHYMGTSTFSEYTVCAEISLAKVN
PQAPLDKVCLLGCGVTTGIGAVHNTAKVKAGDTVAVFGLGGIGLAVIQGAVQAKAGRILAVDTNPEKFKLAAEMGATDFI
NPGDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRAALECCHKGWGESIIIGVAGAGQEIKTRPFQLVTGRVWRGSAFGG
VKGRTQLPGMVEDAMAGKIQLDPFITHRLPLENINEAFDLMHQGKSIRTVIHFGDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=372, Percent_Identity=65.3225806451613, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI34577061, Length=373, Percent_Identity=51.7426273458445, Blast_Score=400, Evalue=1e-112,
Organism=Homo sapiens, GI4501933, Length=373, Percent_Identity=50.6702412868633, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=50.6738544474393, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=49.0666666666667, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI156523966, Length=367, Percent_Identity=49.0463215258856, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=49.3074792243767, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=47.9674796747967, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI71743840, Length=371, Percent_Identity=47.7088948787062, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI156627571, Length=372, Percent_Identity=25.5376344086022, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=80.4878048780488, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=25.6544502617801, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87081918, Length=348, Percent_Identity=32.183908045977, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790045, Length=360, Percent_Identity=27.5, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788073, Length=381, Percent_Identity=26.246719160105, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=26.123595505618, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786825, Length=247, Percent_Identity=30.3643724696356, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI226510992, Length=369, Percent_Identity=25.4742547425474, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788407, Length=230, Percent_Identity=28.2608695652174, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790718, Length=359, Percent_Identity=24.5125348189415, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786518, Length=372, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=59.7855227882037, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=59.4666666666667, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=59.1836734693878, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17562584, Length=364, Percent_Identity=27.7472527472527, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=26.9541778975741, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=373, Percent_Identity=26.5415549597855, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=373, Percent_Identity=60.857908847185, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6323729, Length=355, Percent_Identity=29.2957746478873, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=277, Percent_Identity=31.0469314079422, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=275, Percent_Identity=30.1818181818182, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323099, Length=377, Percent_Identity=28.3819628647215, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=264, Percent_Identity=30.6818181818182, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=304, Percent_Identity=27.3026315789474, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=63.8069705093834, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17137530, Length=347, Percent_Identity=25.9365994236311, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17737897, Length=351, Percent_Identity=24.2165242165242, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 39755; Mature: 39624

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRQMKSRAAVAFGPGQPLSIVEIDVAPPKKGEVLVKITHTGVCHTDAFTLSGDDPEGV
CCHHHHHCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCEEECCEEEECCCCCCCC
FPAVLGHEGGGIVVEVGEGVTSLKPGDHVIPLYTAECGECKFCKSGKTNLCQAVRATQGK
CCCEECCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHCCCC
GLMPDGTTRFSYNGQPIYHYMGTSTFSEYTVCAEISLAKVNPQAPLDKVCLLGCGVTTGI
CCCCCCCEEEEECCCEEEEECCCCCCCCEEEEEEEEEEECCCCCCHHHHHEEECCHHHCC
GAVHNTAKVKAGDTVAVFGLGGIGLAVIQGAVQAKAGRILAVDTNPEKFKLAAEMGATDF
CHHCCCEEEECCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCC
INPGDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRAALECCHKGWGESIIIGVAGAGQE
CCCCCCCCCHHHHHEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
IKTRPFQLVTGRVWRGSAFGGVKGRTQLPGMVEDAMAGKIQLDPFITHRLPLENINEAFD
CCCCCEEEEECEEEECCCCCCCCCCCCCCCHHHHHCCCEEEECCCEECCCCHHHHHHHHH
LMHQGKSIRTVIHFGDK
HHHCCCCEEEEEEECCC
>Mature Secondary Structure 
GKRQMKSRAAVAFGPGQPLSIVEIDVAPPKKGEVLVKITHTGVCHTDAFTLSGDDPEGV
CHHHHHCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCEEECCEEEECCCCCCCC
FPAVLGHEGGGIVVEVGEGVTSLKPGDHVIPLYTAECGECKFCKSGKTNLCQAVRATQGK
CCCEECCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHCCCC
GLMPDGTTRFSYNGQPIYHYMGTSTFSEYTVCAEISLAKVNPQAPLDKVCLLGCGVTTGI
CCCCCCCEEEEECCCEEEEECCCCCCCCEEEEEEEEEEECCCCCCHHHHHEEECCHHHCC
GAVHNTAKVKAGDTVAVFGLGGIGLAVIQGAVQAKAGRILAVDTNPEKFKLAAEMGATDF
CHHCCCEEEECCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCC
INPGDYDKPIQDVIVELTDGGVDFSFECIGNVNVMRAALECCHKGWGESIIIGVAGAGQE
CCCCCCCCCHHHHHEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
IKTRPFQLVTGRVWRGSAFGGVKGRTQLPGMVEDAMAGKIQLDPFITHRLPLENINEAFD
CCCCCEEEEECEEEECCCCCCCCCCCCCCCHHHHHCCCEEEECCCEECCCCHHHHHHHHH
LMHQGKSIRTVIHFGDK
HHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]