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The map label for this gene is catB [H]

Identifier: 218548836

GI number: 218548836

Start: 1516005

End: 1517207

Strand: Direct

Name: catB [H]

Synonym: EFER_1479

Alternate gene names: 218548836

Gene position: 1516005-1517207 (Clockwise)

Preceding gene: 218548834

Following gene: 218548837

Centisome position: 33.04

GC content: 52.2

Gene sequence:

>1203_bases
TTGGTTATCGCTGGCGGTAGAAAAAGTTATGTATTTCATACACAGGAAGAGACGATGAGCGCGACCGTAGAACGCATCGA
ATGTTGGCTGGTAGATTTGCCAACTATTCGCCCCCACAAGCTTTCCATGACCACCATGGGGTGTCAGACACTGGTCATTG
TGCGTCTTACCCGTTCTGACGGTATTACAGGGATAGGGGAAGCGACCACCATCGGTGGCTTAAGTTACGGCGTGGAAAGC
CCGGAGGCCATCGCCTGTGCAATTACAGATTACCTGACTCCCTTGTTAAAAGGGCTGCCAGCCGACAACCTTAATGCGAT
CACCGCGCGAATGAATGCGGCGATCAAAGGGAATACGTTCGCCAAATCAGCGATTGAAACCGCATTGCTTGATGCTCAGG
GTAAAGCACAGGGATTACCTGTTTCGGCACTTCTTGGCGGTGGCTTGTCAACAACCTTGCCTGTCCTCTGGACCCTGGCG
AGTGGTGACACAACAAAAGATATTGCTGAAGGTGAGAATCTGTTGGATCAGGGGCGTCACCAGGCTTTTAAACTCAAAAT
TGGTGCGCGGGAGTTGCAGGCTGATCTCCGGCATACCAGAGCAATTGTTGAGGCACTGGGAGATCGCGCCAGTATTCGCG
TCGATGTTAACCAGGCGTGGAATGCCACCACAGCAGCCAAAGGATGTCGTGAGCTGGCGGCTATGGGCGTGGATCTGATT
GAACAGCCTGTCAGTGCTCAGGATAACGCCGCACTGGTACGTCTGAGCCATCAGGTAGAAACGGCAATTCTTGCTGATGA
AGCGGTGGCGAATCTTTACGACGGTTATCAACTGGCTCAACAAGGATTTAGTGGTGCGTATGCGCTGAAAATTGCCAAAG
CAGGTGGTCCAAATAGTGTGCTGGCACTGGCTCGCGTGGCGCAAGCGGCAGGTATTGGGCTGTATGGCGGTACCATGCTG
GAAGGCACTGTTGGCACCGTCGCCTCTTTGCACGCCTGGTCAACACTACCGTTGCAGTGGGGCACAGAAATGTTCGGTCC
GCTGCTGTTAAAAGATGACATCGTTAGTGTACCGCTCACTTTTGCTGATGGTCAGGTAACACTCCCCCAAACACCAGGAC
TGGGTGTGGAGCTGGATGAAGACAAATTGCGTTTCTATTCCCGTAAAAGAAACGCGGTAACAGGAGAGAACCATGTTATT
TAA

Upstream 100 bases:

>100_bases
TAACCTCAAGTTAACATTTGTGCCGATTATCGCACAACATTTCGATTATCGCCGTTGACCATCTTGCCTGCTCGCTCCTA
TTGTCAAAGCACAACCACCG

Downstream 100 bases:

>100_bases
AGTCGATATGACTGTGAATATTCCACGTGATATTCCTTTTGCTGAAATCGAAGATATAAAAAAACGTGAAAAAGATTATT
CACAACAATTACAACGTGAA

Product: Muconate cycloisomerase I

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MVIAGGRKSYVFHTQEETMSATVERIECWLVDLPTIRPHKLSMTTMGCQTLVIVRLTRSDGITGIGEATTIGGLSYGVES
PEAIACAITDYLTPLLKGLPADNLNAITARMNAAIKGNTFAKSAIETALLDAQGKAQGLPVSALLGGGLSTTLPVLWTLA
SGDTTKDIAEGENLLDQGRHQAFKLKIGARELQADLRHTRAIVEALGDRASIRVDVNQAWNATTAAKGCRELAAMGVDLI
EQPVSAQDNAALVRLSHQVETAILADEAVANLYDGYQLAQQGFSGAYALKIAKAGGPNSVLALARVAQAAGIGLYGGTML
EGTVGTVASLHAWSTLPLQWGTEMFGPLLLKDDIVSVPLTFADGQVTLPQTPGLGVELDEDKLRFYSRKRNAVTGENHVI

Sequences:

>Translated_400_residues
MVIAGGRKSYVFHTQEETMSATVERIECWLVDLPTIRPHKLSMTTMGCQTLVIVRLTRSDGITGIGEATTIGGLSYGVES
PEAIACAITDYLTPLLKGLPADNLNAITARMNAAIKGNTFAKSAIETALLDAQGKAQGLPVSALLGGGLSTTLPVLWTLA
SGDTTKDIAEGENLLDQGRHQAFKLKIGARELQADLRHTRAIVEALGDRASIRVDVNQAWNATTAAKGCRELAAMGVDLI
EQPVSAQDNAALVRLSHQVETAILADEAVANLYDGYQLAQQGFSGAYALKIAKAGGPNSVLALARVAQAAGIGLYGGTML
EGTVGTVASLHAWSTLPLQWGTEMFGPLLLKDDIVSVPLTFADGQVTLPQTPGLGVELDEDKLRFYSRKRNAVTGENHVI
>Mature_400_residues
MVIAGGRKSYVFHTQEETMSATVERIECWLVDLPTIRPHKLSMTTMGCQTLVIVRLTRSDGITGIGEATTIGGLSYGVES
PEAIACAITDYLTPLLKGLPADNLNAITARMNAAIKGNTFAKSAIETALLDAQGKAQGLPVSALLGGGLSTTLPVLWTLA
SGDTTKDIAEGENLLDQGRHQAFKLKIGARELQADLRHTRAIVEALGDRASIRVDVNQAWNATTAAKGCRELAAMGVDLI
EQPVSAQDNAALVRLSHQVETAILADEAVANLYDGYQLAQQGFSGAYALKIAKAGGPNSVLALARVAQAAGIGLYGGTML
EGTVGTVASLHAWSTLPLQWGTEMFGPLLLKDDIVSVPLTFADGQVTLPQTPGLGVELDEDKLRFYSRKRNAVTGENHVI

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=332, Percent_Identity=30.421686746988, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994953, Length=363, Percent_Identity=24.5179063360882, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 42271; Mature: 42271

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIAGGRKSYVFHTQEETMSATVERIECWLVDLPTIRPHKLSMTTMGCQTLVIVRLTRSD
CEEECCCCCEEEECHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCEEEEEEEEECCC
GITGIGEATTIGGLSYGVESPEAIACAITDYLTPLLKGLPADNLNAITARMNAAIKGNTF
CCCCCCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCEECCCHH
AKSAIETALLDAQGKAQGLPVSALLGGGLSTTLPVLWTLASGDTTKDIAEGENLLDQGRH
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHCCC
QAFKLKIGARELQADLRHTRAIVEALGDRASIRVDVNQAWNATTAAKGCRELAAMGVDLI
EEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCHHHH
EQPVSAQDNAALVRLSHQVETAILADEAVANLYDGYQLAQQGFSGAYALKIAKAGGPNSV
HCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LALARVAQAAGIGLYGGTMLEGTVGTVASLHAWSTLPLQWGTEMFGPLLLKDDIVSVPLT
HHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHCEECCHHCCHHHHCCEEECCCCEEEEEE
FADGQVTLPQTPGLGVELDEDKLRFYSRKRNAVTGENHVI
ECCCEEECCCCCCCCCEECHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVIAGGRKSYVFHTQEETMSATVERIECWLVDLPTIRPHKLSMTTMGCQTLVIVRLTRSD
CEEECCCCCEEEECHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCEEEEEEEEECCC
GITGIGEATTIGGLSYGVESPEAIACAITDYLTPLLKGLPADNLNAITARMNAAIKGNTF
CCCCCCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCEECCCHH
AKSAIETALLDAQGKAQGLPVSALLGGGLSTTLPVLWTLASGDTTKDIAEGENLLDQGRH
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHCCC
QAFKLKIGARELQADLRHTRAIVEALGDRASIRVDVNQAWNATTAAKGCRELAAMGVDLI
EEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCHHHH
EQPVSAQDNAALVRLSHQVETAILADEAVANLYDGYQLAQQGFSGAYALKIAKAGGPNSV
HCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LALARVAQAAGIGLYGGTMLEGTVGTVASLHAWSTLPLQWGTEMFGPLLLKDDIVSVPLT
HHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHCEECCHHCCHHHHCCEEECCCCEEEEEE
FADGQVTLPQTPGLGVELDEDKLRFYSRKRNAVTGENHVI
ECCCEEECCCCCCCCCEECHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]