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The map label for this gene is ynfK [H]

Identifier: 218548804

GI number: 218548804

Start: 1485663

End: 1486358

Strand: Direct

Name: ynfK [H]

Synonym: EFER_1443

Alternate gene names: 218548804

Gene position: 1485663-1486358 (Clockwise)

Preceding gene: 218548803

Following gene: 218548810

Centisome position: 32.38

GC content: 49.71

Gene sequence:

>696_bases
ATGCTAAAGCGTTTCTTTATTACCGGTACAGACACTTCTGTTGGAAAAACGGTAGTTTCCCGCGCATTGCTACAGGCGTT
AGCATCGGAGGGTAAAGTAGTCGCGGGTTATAAACCCGTGGCCAAGGGGAGCAAAGAGACAGCGGAAGGCCTACGCAACA
AAGATGCGTTGGTATTACAAAGCGTCTCAACCATTTCATTGCCTTATGAGGCAGTAAATCCCATTGCGCTAAGTGAAGAA
GAAAGTAGCGTGGCGCACAGCTGCCCGATCAACTACACCTTGATCTCCAACGGTCTTGCTAAGCTGACGGAAAAGGTGGA
ACACGTCGTGGTTGAAGGCACGGGGGGATGGCGTAGTTTGATGAATGATCTACGCCCTTTGTCAGAATGGGTAGTGCAAG
AACAGTTACCCGTGGTGATGGTCGTCGGTATCCAGGAAGGTTGTATAAACCACGCCTTACTGACGGCGCAGGCCATTGCC
AATGACGGCCTGCCGCTAATTGGCTGGGTAGCAAATCGCATTAACCCAGGTCTGGCGCACTATGCCGAAATTATTGATGT
GCTGAGCAAAAAAATTCCTGCGCCATTGATTGGTGAATTGCCTTATCTGCCTCGTGCTGAGCAACGCGAACTGGGGCAAT
ATATTCGTTTATCTATGCTCGACCGGGTGTTGTCGGTAAATAGAATCGGGATGTAA

Upstream 100 bases:

>100_bases
TTTGCGCTAACTCAATCTGAATCTCATCGCCTTAACTTAGACTTTTCTATCGTTTTATTAATCTGGCTTATATTTTCGAA
GCATAACGGTGGAGTTAGTG

Downstream 100 bases:

>100_bases
CGATCGCGAAATCACCGTCGCTAATACACATGCCAGCAATAAACCGGGGAGTAGTTGATACTCTCCGGTCATTTCGCAAA
TCATTAATGTCGACATAATT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASEGKVVAGYKPVAKGSKETAEGLRNKDALVLQSVSTISLPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLAKLTEKVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVVMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKIPAPLIGELPYLPRAEQRELGQYIRLSMLDRVLSVNRIGM

Sequences:

>Translated_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASEGKVVAGYKPVAKGSKETAEGLRNKDALVLQSVSTISLPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLAKLTEKVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVVMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKIPAPLIGELPYLPRAEQRELGQYIRLSMLDRVLSVNRIGM
>Mature_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASEGKVVAGYKPVAKGSKETAEGLRNKDALVLQSVSTISLPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLAKLTEKVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVVMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKIPAPLIGELPYLPRAEQRELGQYIRLSMLDRVLSVNRIGM

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=229, Percent_Identity=93.4497816593886, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=50.2222222222222, Blast_Score=220, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25017; Mature: 25017

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASEGKVVAGYKPVAKGSKETAEGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCHHHHH
SVSTISLPYEAVNPIALSEEESSVAHSCPINYTLISNGLAKLTEKVEHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVVMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLSKKIPAPLIGELPYLPRAEQRELGQYIRLSMLDRVLSVNRIGM
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASEGKVVAGYKPVAKGSKETAEGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCHHHHH
SVSTISLPYEAVNPIALSEEESSVAHSCPINYTLISNGLAKLTEKVEHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVVMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLSKKIPAPLIGELPYLPRAEQRELGQYIRLSMLDRVLSVNRIGM
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]