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The map label for this gene is pntA [H]

Identifier: 218548795

GI number: 218548795

Start: 1474735

End: 1476279

Strand: Direct

Name: pntA [H]

Synonym: EFER_1434

Alternate gene names: 218548795

Gene position: 1474735-1476279 (Clockwise)

Preceding gene: 218548791

Following gene: 218548796

Centisome position: 32.14

GC content: 50.49

Gene sequence:

>1545_bases
ATGGAAGGGAATATTATGCGAATTGGCATACCAAAAGAGCGGTTAACCAATGAAACCAGAGTCGCTGCAACACCAAAAAC
GGTTGAGCAGCTACTGAAACTAGGTTTCACCGTCGCAGTAGAGAGCGGCGCGGGTCAACTGGCAAGTTTTGACGATAAGG
CATTTGAACACGCAGGGGCGGAAATAGTCGATGGCAACGCCATTTGGCAGTCAGAGATTATTCTCAAAGTAAACGCACCT
GAAGATGAAGAAATTCCGCTACTCAAAGCGGGAACAACACTGGTTAGCTTCATCTGGCCTGCACAAAATCCTGAACTGAT
GCAAAAACTGGCGGAGCGCGACGTGACCGTCATGGCAATGGACTCTGTGCCACGTATCTCACGTGCGCAGTCGCTGGATG
CATTAAGCTCAATGGCGAATATCGCGGGTTACCGCGCGATTGTCGAAGCCGCACATGAATTTGGCCGCTTCTTTACCGGG
CAAATTACGGCTGCTGGGAAAGTGCCTCCGGCCAAAGTTATGGTCATTGGCGCTGGGGTTGCGGGCCTTGCGGCTATCGG
CGCGGCTAACAGTTTGGGGGCGATTGTTCGCGCCTTTGACACCCGCCCGGAAGTAAAAGAACAAGTACAGAGTATGGGCG
CCGAGTTCCTCGAGCTTGATTTCAAAGAGGAAGCAGGCAGTGGCGATGGTTATGCCAAAGTAATGTCTGAGGCGTTTATC
AAAGCCGAAATGGAACTCTTTGCTGCTCAGGCAAAAGATGTCGATATCATCGTTACTACCGCACTTATTCCTGGCAAGCC
TGCACCAAAACTTATCACCCGTGAAATGGTTGACTCCATGAAATCGGGCAGTGTGATTGTCGATCTGGCGGCCCAGAACG
GGGGTAACTGCGAATACACCGTACCAGGCGAAATTTATACTACTGAAAACGGCGTAAAAGTGATTGGCTACACCGATCTG
CCAGGTCGCTTGCCGACCCAATCTTCACAGCTTTACGGTACTAACCTGGTCAACCTGCTGAAACTGCTTTGCAAAGAGAA
AGACGGCAACATTACTGTTGATTTCGATGATGTCGTGATTCGTGGTGTGACGGTGATCCGCGATGGAGAAATTACCTGGC
CGGCACCACCGATTCAGGTTTCCGCTCAGCCACAGACAGCACCGAAAGCCGCGCCCCAACCAACGGAACCGGAAAAACCG
GCTTCGCCATGGCGGAAATATGCCTTGATGGCTTTGGCCATTATTCTCTTTGGCTGGATGGCAGATGTCGCGCCTAAAGA
GTTCCTTGGTCACTTCACCGTTTTTGCCCTTGCATGTGTCGTTGGTTATTACGTGGTCTGGAACGTTTCTCACGCGTTAC
ATACACCGCTGATGTCTGTCACTAACGCAATTTCGGGGATTATTGTTGTCGGAGCATTGTTGCAAATAGGCCAGGGTGGC
TGGGTTAGCTTCCTGAGCTTTATTGCGGTGCTTATCGCCAGCATTAATATTTTCGGTGGTTTCACTGTAACTCAGCGTAT
GCTGAAAATGTTCCGCAAAAATTAA

Upstream 100 bases:

>100_bases
ATAGCAAGTTTGTTACAGTTTTTTTCCGGGGTAATACATACCTCTAACGTAAGAGCGCTTTGGCCTTTTACGATCACTGA
CAAACCATCATCCAAAACCG

Downstream 100 bases:

>100_bases
GGGGTAACATATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCAATCCTGTTTATTTTTAGTCTGGCGGGGC
TTTCAAAACACGAAACTTCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MEGNIMRIGIPKERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFEHAGAEIVDGNAIWQSEIILKVNAP
EDEEIPLLKAGTTLVSFIWPAQNPELMQKLAERDVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
KAEMELFAAQAKDVDIIVTTALIPGKPAPKLITREMVDSMKSGSVIVDLAAQNGGNCEYTVPGEIYTTENGVKVIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRDGEITWPAPPIQVSAQPQTAPKAAPQPTEPEKP
ASPWRKYALMALAIILFGWMADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGG
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_514_residues
MEGNIMRIGIPKERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFEHAGAEIVDGNAIWQSEIILKVNAP
EDEEIPLLKAGTTLVSFIWPAQNPELMQKLAERDVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
KAEMELFAAQAKDVDIIVTTALIPGKPAPKLITREMVDSMKSGSVIVDLAAQNGGNCEYTVPGEIYTTENGVKVIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRDGEITWPAPPIQVSAQPQTAPKAAPQPTEPEKP
ASPWRKYALMALAIILFGWMADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGG
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_514_residues
MEGNIMRIGIPKERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFEHAGAEIVDGNAIWQSEIILKVNAP
EDEEIPLLKAGTTLVSFIWPAQNPELMQKLAERDVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
KAEMELFAAQAKDVDIIVTTALIPGKPAPKLITREMVDSMKSGSVIVDLAAQNGGNCEYTVPGEIYTTENGVKVIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRDGEITWPAPPIQVSAQPQTAPKAAPQPTEPEKP
ASPWRKYALMALAIILFGWMADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGG
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=538, Percent_Identity=51.6728624535316, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI122939153, Length=538, Percent_Identity=51.6728624535316, Blast_Score=498, Evalue=1e-141,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=94.5098039215686, Blast_Score=954, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=526, Percent_Identity=51.1406844106464, Blast_Score=493, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 55138; Mature: 55138

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGNIMRIGIPKERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFEHAGA
CCCCEEEECCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCC
EIVDGNAIWQSEIILKVNAPEDEEIPLLKAGTTLVSFIWPAQNPELMQKLAERDVTVMAM
EEECCCEEEEEEEEEEECCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHCCEEEEEE
DSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCH
AGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCHHHHHHHHHHH
KAEMELFAAQAKDVDIIVTTALIPGKPAPKLITREMVDSMKSGSVIVDLAAQNGGNCEYT
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
VPGEIYTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVI
ECCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHEEE
RGVTVIRDGEITWPAPPIQVSAQPQTAPKAAPQPTEPEKPASPWRKYALMALAIILFGWM
ECEEEEECCEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGNIMRIGIPKERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFEHAGA
CCCCEEEECCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCC
EIVDGNAIWQSEIILKVNAPEDEEIPLLKAGTTLVSFIWPAQNPELMQKLAERDVTVMAM
EEECCCEEEEEEEEEEECCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHCCEEEEEE
DSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCH
AGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFI
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCHHHHHHHHHHH
KAEMELFAAQAKDVDIIVTTALIPGKPAPKLITREMVDSMKSGSVIVDLAAQNGGNCEYT
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
VPGEIYTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVI
ECCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHEEE
RGVTVIRDGEITWPAPPIQVSAQPQTAPKAAPQPTEPEKPASPWRKYALMALAIILFGWM
ECEEEEECCEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ADVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]