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The map label for this gene is rstB [H]

Identifier: 218548789

GI number: 218548789

Start: 1468478

End: 1469764

Strand: Reverse

Name: rstB [H]

Synonym: EFER_1428

Alternate gene names: 218548789

Gene position: 1469764-1468478 (Counterclockwise)

Preceding gene: 218548790

Following gene: 218548788

Centisome position: 32.03

GC content: 49.11

Gene sequence:

>1287_bases
ATGAAAAAACTTTTTGTTCAGTTTTATCTGCTTCTCTTCACCTGTTTTCTGGTAATGACCTTGTTAGTGGGGCTGGTATA
TAAATTCACCGCTGAACGCGCCGGTAAGCAGTCCCTGGATGATTTAATGAAAAGCTCCCTGTATCTGATGCGTAGCGAGC
TGCGGGAAATTCCCCCTCGCGAATGGGGAAAAACCTTAAAAGAGATGGACCTGAATCTCTCTTTCGACCTTCGTGTAGAA
CCATTGCAATATTACCAGCTTGAGCCCCGTGCAATGCAGCGTTTACGCAGTGGTGAAATTGTGGCGCTTGAGGACCAATA
TACATTTATCCAGCGTATTCCCCGCAGCCACTACGTTCTGGCTGTCGGCCCTGTGCCCTATCTTTATTTCCTGCACCAGA
TGCGCTTACTTGATGTGGCGCTAATTGCTTTTATCGCCTTCTCTCTCGCCTTTCCGGTGTTTATCTGGATGCGCCCACAC
TGGCAGGATATGTTGAAGCTGGAAGCGGCAGCGCAACGATTTGGCGAAGGCCATCTGACCGAACGATTGCATTTTGACAG
TAACTCCAGTTTTGCCCGCCTTGGCGTGGCCTTTAACCAAATGGCTGACAACATTAACGCTCTGATTGTCAGCAAAAAGC
AGCTAATCGATGGTATTGCCCACGAGCTGCGTACCCCTCTGGTACGATTACGCTACAGGCTGGAAATGAGCGATAACCTG
AGTGAAGCAGAATCGCTGGCGCTAAATCGCGATATTGGTCAACTGGAGTCATTGATTGAAGAGCTGCTCACTTATGCCCG
GCTGGACAGACCGCAAAATGAGCTAAGCCTCAGTGAAGTGGATCTCCCCCACTGGTTAACGACGCATTTGGATGATGTGC
AAACCTTGAATCCAGACCATAACATTCGGGTGAAAACGCTGAATACGGCAAATTATGGCGTTCTGGATATGCGGTTAATG
GAAAGAGTACTGGATAATTTAGTTAATAATGCCCTGCGTTACTGCTGTTCGACAGTGGAAGTTAGTCTGATACATCAGGG
TAACTTCGCAACACTCATTGTTGAAGATGATGGGCCGGGTATCGCCCAGGACGCCCGTGAACATATCTTCGACCCCTTTG
TTCGCCTTGATCCCAGCCGTGACCGGAGCACGGGGGGATGTGGTCTGGGATTGGCGATTGTACGCTCCATTGCCCAGGCA
ATGGGCGGAACGGTCATCTGTGAAGAAAGCGAATCAGGTGGTGCGAAATTTAGTTTCAACTGGCAAGTGTTATCTCTATC
AGCCTGA

Upstream 100 bases:

>100_bases
GAAATTACTCGATAACGCCGCCGAACCTTACCGGATCAAAACTATTCGTAATAAAGGTTATTTGTTCGCGCCACACGCCT
GGGAATAAACGGGTTAAGAT

Downstream 100 bases:

>100_bases
AATAATTCGAATTGCCGGGCAAATTACCTATAGCATGTTGAACACTGCCTGGAAATAAATGCATAAATCCCTGCCAGACC
ATGACCGCACGGTGGTGATG

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKKLFVQFYLLLFTCFLVMTLLVGLVYKFTAERAGKQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLQYYQLEPRAMQRLRSGEIVALEDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDVALIAFIAFSLAFPVFIWMRPH
WQDMLKLEAAAQRFGEGHLTERLHFDSNSSFARLGVAFNQMADNINALIVSKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SEAESLALNRDIGQLESLIEELLTYARLDRPQNELSLSEVDLPHWLTTHLDDVQTLNPDHNIRVKTLNTANYGVLDMRLM
ERVLDNLVNNALRYCCSTVEVSLIHQGNFATLIVEDDGPGIAQDAREHIFDPFVRLDPSRDRSTGGCGLGLAIVRSIAQA
MGGTVICEESESGGAKFSFNWQVLSLSA

Sequences:

>Translated_428_residues
MKKLFVQFYLLLFTCFLVMTLLVGLVYKFTAERAGKQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLQYYQLEPRAMQRLRSGEIVALEDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDVALIAFIAFSLAFPVFIWMRPH
WQDMLKLEAAAQRFGEGHLTERLHFDSNSSFARLGVAFNQMADNINALIVSKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SEAESLALNRDIGQLESLIEELLTYARLDRPQNELSLSEVDLPHWLTTHLDDVQTLNPDHNIRVKTLNTANYGVLDMRLM
ERVLDNLVNNALRYCCSTVEVSLIHQGNFATLIVEDDGPGIAQDAREHIFDPFVRLDPSRDRSTGGCGLGLAIVRSIAQA
MGGTVICEESESGGAKFSFNWQVLSLSA
>Mature_428_residues
MKKLFVQFYLLLFTCFLVMTLLVGLVYKFTAERAGKQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRVE
PLQYYQLEPRAMQRLRSGEIVALEDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDVALIAFIAFSLAFPVFIWMRPH
WQDMLKLEAAAQRFGEGHLTERLHFDSNSSFARLGVAFNQMADNINALIVSKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SEAESLALNRDIGQLESLIEELLTYARLDRPQNELSLSEVDLPHWLTTHLDDVQTLNPDHNIRVKTLNTANYGVLDMRLM
ERVLDNLVNNALRYCCSTVEVSLIHQGNFATLIVEDDGPGIAQDAREHIFDPFVRLDPSRDRSTGGCGLGLAIVRSIAQA
MGGTVICEESESGGAKFSFNWQVLSLSA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=421, Percent_Identity=83.6104513064133, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=261, Percent_Identity=34.4827586206897, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789808, Length=266, Percent_Identity=32.3308270676692, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788393, Length=262, Percent_Identity=28.2442748091603, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786783, Length=235, Percent_Identity=29.3617021276596, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=27.6497695852535, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789403, Length=235, Percent_Identity=31.9148936170213, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787374, Length=95, Percent_Identity=40, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=29.6442687747036, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790551, Length=232, Percent_Identity=28.448275862069, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=30.3571428571429, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=283, Percent_Identity=28.6219081272085, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082128, Length=255, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=26.2222222222222, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=27.3127753303965, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=26.2008733624454, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=22.9838709677419, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48889; Mature: 48889

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFVQFYLLLFTCFLVMTLLVGLVYKFTAERAGKQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
EWGKTLKEMDLNLSFDLRVEPLQYYQLEPRAMQRLRSGEIVALEDQYTFIQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCHHHEECCHHHHHHHCCCCEEEEECCHHHHHHCCCCCEEE
AVGPVPYLYFLHQMRLLDVALIAFIAFSLAFPVFIWMRPHWQDMLKLEAAAQRFGEGHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCH
ERLHFDSNSSFARLGVAFNQMADNINALIVSKKQLIDGIAHELRTPLVRLRYRLEMSDNL
HEEECCCCCCHHHHHHHHHHHHCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SEAESLALNRDIGQLESLIEELLTYARLDRPQNELSLSEVDLPHWLTTHLDDVQTLNPDH
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHCCCCHHHHHHHHHHHHCCCCC
NIRVKTLNTANYGVLDMRLMERVLDNLVNNALRYCCSTVEVSLIHQGNFATLIVEDDGPG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCC
IAQDAREHIFDPFVRLDPSRDRSTGGCGLGLAIVRSIAQAMGGTVICEESESGGAKFSFN
HHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
WQVLSLSA
EEEEEECC
>Mature Secondary Structure
MKKLFVQFYLLLFTCFLVMTLLVGLVYKFTAERAGKQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
EWGKTLKEMDLNLSFDLRVEPLQYYQLEPRAMQRLRSGEIVALEDQYTFIQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCHHHEECCHHHHHHHCCCCEEEEECCHHHHHHCCCCCEEE
AVGPVPYLYFLHQMRLLDVALIAFIAFSLAFPVFIWMRPHWQDMLKLEAAAQRFGEGHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCH
ERLHFDSNSSFARLGVAFNQMADNINALIVSKKQLIDGIAHELRTPLVRLRYRLEMSDNL
HEEECCCCCCHHHHHHHHHHHHCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SEAESLALNRDIGQLESLIEELLTYARLDRPQNELSLSEVDLPHWLTTHLDDVQTLNPDH
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHCCCCHHHHHHHHHHHHCCCCC
NIRVKTLNTANYGVLDMRLMERVLDNLVNNALRYCCSTVEVSLIHQGNFATLIVEDDGPG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCC
IAQDAREHIFDPFVRLDPSRDRSTGGCGLGLAIVRSIAQAMGGTVICEESESGGAKFSFN
HHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
WQVLSLSA
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]