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The map label for this gene is btuC [H]

Identifier: 218548717

GI number: 218548717

Start: 1388889

End: 1389869

Strand: Direct

Name: btuC [H]

Synonym: EFER_1354

Alternate gene names: 218548717

Gene position: 1388889-1389869 (Clockwise)

Preceding gene: 218548716

Following gene: 218548718

Centisome position: 30.27

GC content: 50.66

Gene sequence:

>981_bases
ATGCTGACATTTGCCCGCCAACAACAGCGAAAAAACATTCGCTGGCTCTTTTGTCTGTCAGCATTGATGCTGCTGACGCT
TGTCTTAAGTCTTTGCGCAGGTGAACAATGGATTTTACCCGCTGACTGGTTTTCTCCTCAGGGTGAGCTTTTCGTCTGGC
AAATTCGTCTGCCACGTACGCTGGCAGTACTACTGGTCGGCGCGGCCCTGGCGTTGTCCGGTGCCGTAATGCAAGCACTT
TTCGAAAATCCACTGGCAGAGCCTGGCTTACTCGGCGTCTCTAATGGTGCAGGAGTAGGTCTTATTGCTGCTGTATTCTT
AGGGCAGGGGCAATTGCCCGGTTGGGCATTAGGATTGTGTGCGATTGCGGGAGCTTTAATAATTACTTTGATTCTTCTGC
GTTTTGCCCGCCGTCATCTCTCCACAAGTCGCCTTTTATTAGCAGGCGTAGCTTTGGGAATCATATGCAGCGCTATGATG
ACCTGGGCAATCTATTTCTCAACGTCATTCGATTTACGCCAGCTAATGTACTGGATGATGGGCGGGTTTGGCGGCGTAAA
CTGGCAACAAGCCTGGTTAATGGTAGCGCTTATACCGGTGTTACTGTGGATCTGTTGCCAGTCACAGGCAATGAATATGC
TGGCACTGGGGGAAACTTCTGCACGTCAATTGGGATTACCTTTGTGGTTCTGGCGTAACGTGCTGGTGGTAGCTACGGGA
TGGATGGTAGGTGTGAGTGTAGCACTGGCGGGGGCAATTGGTTTTATCGGCCTTGTGATCCCTCATATTTTACGTTTATG
CGGGTTAACAGATCATCGGATGTTATTACCTGGCTGTGCGCTGGCAGGAGCAATTGCATTATTGCTGGCAGACGTTGTCG
CACGAGTTGCGCTTGTCGCCGCAGAATTACCGATTGGTGTAGTGACGGCTACGCTGGGCGCTCCAGTTTTCATTTGGTTA
TTGCTGAAAAGTCGGGCGTAA

Upstream 100 bases:

>100_bases
TCTGATCTAACTAAAAAGGCCGCTTTGCGGCCTTTTTTCTTTTCACTCTTGAAGAGTCACCGTAAAATCAACGCCATCAC
TCTTCAGCTGAATGGATACT

Downstream 100 bases:

>100_bases
GATATGACGCAATTAATGCTATTAGAAGATGTTGCCGAGTCTCACCGCCTGGGGCCGCTTTCTGGTGAAATTCGTGCAGG
TGAAATCCTCCATCTTGTTG

Product: vtamin B12-transporter permease

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLTFARQQQRKNIRWLFCLSALMLLTLVLSLCAGEQWILPADWFSPQGELFVWQIRLPRTLAVLLVGAALALSGAVMQAL
FENPLAEPGLLGVSNGAGVGLIAAVFLGQGQLPGWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSAMM
TWAIYFSTSFDLRQLMYWMMGGFGGVNWQQAWLMVALIPVLLWICCQSQAMNMLALGETSARQLGLPLWFWRNVLVVATG
WMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRMLLPGCALAGAIALLLADVVARVALVAAELPIGVVTATLGAPVFIWL
LLKSRA

Sequences:

>Translated_326_residues
MLTFARQQQRKNIRWLFCLSALMLLTLVLSLCAGEQWILPADWFSPQGELFVWQIRLPRTLAVLLVGAALALSGAVMQAL
FENPLAEPGLLGVSNGAGVGLIAAVFLGQGQLPGWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSAMM
TWAIYFSTSFDLRQLMYWMMGGFGGVNWQQAWLMVALIPVLLWICCQSQAMNMLALGETSARQLGLPLWFWRNVLVVATG
WMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRMLLPGCALAGAIALLLADVVARVALVAAELPIGVVTATLGAPVFIWL
LLKSRA
>Mature_326_residues
MLTFARQQQRKNIRWLFCLSALMLLTLVLSLCAGEQWILPADWFSPQGELFVWQIRLPRTLAVLLVGAALALSGAVMQAL
FENPLAEPGLLGVSNGAGVGLIAAVFLGQGQLPGWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSAMM
TWAIYFSTSFDLRQLMYWMMGGFGGVNWQQAWLMVALIPVLLWICCQSQAMNMLALGETSARQLGLPLWFWRNVLVVATG
WMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRMLLPGCALAGAIALLLADVVARVALVAAELPIGVVTATLGAPVFIWL
LLKSRA

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane [H]

COG id: COG4139

COG function: function code H; ABC-type cobalamin transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788004, Length=324, Percent_Identity=91.0493827160494, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1790740, Length=292, Percent_Identity=38.3561643835616, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790741, Length=315, Percent_Identity=32.3809523809524, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786805, Length=287, Percent_Identity=40.418118466899, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786347, Length=280, Percent_Identity=31.7857142857143, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786804, Length=280, Percent_Identity=33.5714285714286, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 35038; Mature: 35038

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFARQQQRKNIRWLFCLSALMLLTLVLSLCAGEQWILPADWFSPQGELFVWQIRLPRT
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEEEECHHH
LAVLLVGAALALSGAVMQALFENPLAEPGLLGVSNGAGVGLIAAVFLGQGQLPGWALGLC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCHHHHHHH
AIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSAMMTWAIYFSTSFDLRQLMYWMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GGFGGVNWQQAWLMVALIPVLLWICCQSQAMNMLALGETSARQLGLPLWFWRNVLVVATG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCHHHHHHHHHHHHHH
WMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRMLLPGCALAGAIALLLADVVARVALVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELPIGVVTATLGAPVFIWLLLKSRA
HHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTFARQQQRKNIRWLFCLSALMLLTLVLSLCAGEQWILPADWFSPQGELFVWQIRLPRT
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEEEECHHH
LAVLLVGAALALSGAVMQALFENPLAEPGLLGVSNGAGVGLIAAVFLGQGQLPGWALGLC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCHHHHHHH
AIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSAMMTWAIYFSTSFDLRQLMYWMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GGFGGVNWQQAWLMVALIPVLLWICCQSQAMNMLALGETSARQLGLPLWFWRNVLVVATG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCHHHHHHHHHHHHHH
WMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRMLLPGCALAGAIALLLADVVARVALVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELPIGVVTATLGAPVFIWLLLKSRA
HHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA