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The map label for this gene is btuD [H]

Identifier: 218548718

GI number: 218548718

Start: 1389873

End: 1390622

Strand: Direct

Name: btuD [H]

Synonym: EFER_1355

Alternate gene names: 218548718

Gene position: 1389873-1390622 (Clockwise)

Preceding gene: 218548717

Following gene: 218548719

Centisome position: 30.29

GC content: 49.87

Gene sequence:

>750_bases
ATGACGCAATTAATGCTATTAGAAGATGTTGCCGAGTCTCACCGCCTGGGGCCGCTTTCTGGTGAAATTCGTGCAGGTGA
AATCCTCCATCTTGTTGGACCAAATGGTGCCGGGAAAAGTACTTTACTGGCACGGATGGCAGGTTTGACTACGGGTCCTG
GTCGTGTTTATTTCGCCGGTTCTGTACTGGAATCCTGGTCAGCTACAAAGCTGGCACTGCATCGGGCTTACCTTGCCCAG
CAACAAAATCCACCTTTTGCAATGCCTGTCTGGCATTTTTTATCTCTACATCAGCCAGATAAGACACGCACTGACTTACT
CACCAAAATGGCTGAATCGTTAGGATTAGCGGATAAACTTGAACGTCCGGCTAATCAACTCTCGGGCGGCGAGTGGCAAC
GCGTGCGCCTGGCCGCGGTTGTGCTACAAATTGACCCGAAATCGAATCCACACGGTCAGTTGTTATTGCTAGACGAACCG
ATGAATAGTCTCGATGTCGCACAACAAAACGCGCTGGATCGCATATTGAGTCATCTTTGTCAGCAGGGTATCGCTGTGGT
GATGAGTAGTCACGATCTCAACCACACGCTACACCATGCTCACAGGGCATGGCTGCTAAAACAAGGCAAACTTCTGGCTG
GTGGCCCACGGGAAGATGTGTTGACGCCACCTAATCTGGCATTAGCGTATGGCTTAAATTTCCGTCGCCTTGATATAGAA
GGGCATCGAATGCTCATCTCTACCAGTTAA

Upstream 100 bases:

>100_bases
AGTTGCGCTTGTCGCCGCAGAATTACCGATTGGTGTAGTGACGGCTACGCTGGGCGCTCCAGTTTTCATTTGGTTATTGC
TGAAAAGTCGGGCGTAAGAT

Downstream 100 bases:

>100_bases
TTGAGCCATGACCTTGAAAAACGGCTAAAAACGACGCTAAATTATCTGAAGAATAAAAAAACAGAGGATTGTTCTGAATG
CGATTATGCCTTCTTTTTAT

Product: vitamin B12-transporter ATPase

Products: NA

Alternate protein names: Vitamin B12-transporting ATPase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTQLMLLEDVAESHRLGPLSGEIRAGEILHLVGPNGAGKSTLLARMAGLTTGPGRVYFAGSVLESWSATKLALHRAYLAQ
QQNPPFAMPVWHFLSLHQPDKTRTDLLTKMAESLGLADKLERPANQLSGGEWQRVRLAAVVLQIDPKSNPHGQLLLLDEP
MNSLDVAQQNALDRILSHLCQQGIAVVMSSHDLNHTLHHAHRAWLLKQGKLLAGGPREDVLTPPNLALAYGLNFRRLDIE
GHRMLISTS

Sequences:

>Translated_249_residues
MTQLMLLEDVAESHRLGPLSGEIRAGEILHLVGPNGAGKSTLLARMAGLTTGPGRVYFAGSVLESWSATKLALHRAYLAQ
QQNPPFAMPVWHFLSLHQPDKTRTDLLTKMAESLGLADKLERPANQLSGGEWQRVRLAAVVLQIDPKSNPHGQLLLLDEP
MNSLDVAQQNALDRILSHLCQQGIAVVMSSHDLNHTLHHAHRAWLLKQGKLLAGGPREDVLTPPNLALAYGLNFRRLDIE
GHRMLISTS
>Mature_248_residues
TQLMLLEDVAESHRLGPLSGEIRAGEILHLVGPNGAGKSTLLARMAGLTTGPGRVYFAGSVLESWSATKLALHRAYLAQQ
QNPPFAMPVWHFLSLHQPDKTRTDLLTKMAESLGLADKLERPANQLSGGEWQRVRLAAVVLQIDPKSNPHGQLLLLDEPM
NSLDVAQQNALDRILSHLCQQGIAVVMSSHDLNHTLHHAHRAWLLKQGKLLAGGPREDVLTPPNLALAYGLNFRRLDIEG
HRMLISTS

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Responsible for energy coupling to the transport system [H]

COG id: COG4138

COG function: function code H; ABC-type cobalamin transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI46592956, Length=211, Percent_Identity=28.9099526066351, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI46592915, Length=211, Percent_Identity=28.9099526066351, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI46592971, Length=211, Percent_Identity=28.9099526066351, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI46592978, Length=208, Percent_Identity=28.8461538461538, Blast_Score=64, Evalue=9e-11,
Organism=Homo sapiens, GI46592964, Length=211, Percent_Identity=28.9099526066351, Blast_Score=64, Evalue=1e-10,
Organism=Homo sapiens, GI46592898, Length=211, Percent_Identity=28.9099526066351, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1788002, Length=248, Percent_Identity=79.8387096774193, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1786803, Length=231, Percent_Identity=30.3030303030303, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1786345, Length=230, Percent_Identity=32.1739130434783, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790739, Length=221, Percent_Identity=31.2217194570136, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788165, Length=234, Percent_Identity=32.4786324786325, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789593, Length=226, Percent_Identity=28.7610619469027, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081791, Length=234, Percent_Identity=28.6324786324786, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081782, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787370, Length=214, Percent_Identity=29.4392523364486, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788506, Length=221, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787105, Length=241, Percent_Identity=29.4605809128631, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786563, Length=194, Percent_Identity=29.8969072164948, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789891, Length=213, Percent_Identity=30.0469483568075, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788761, Length=236, Percent_Identity=30.0847457627119, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790544, Length=227, Percent_Identity=27.7533039647577, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789963, Length=218, Percent_Identity=28.4403669724771, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87081834, Length=220, Percent_Identity=29.5454545454545, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786253, Length=208, Percent_Identity=27.4038461538462, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787759, Length=247, Percent_Identity=28.7449392712551, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786872, Length=204, Percent_Identity=26.9607843137255, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787758, Length=219, Percent_Identity=26.9406392694064, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787792, Length=224, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788450, Length=213, Percent_Identity=29.5774647887324, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081709, Length=235, Percent_Identity=24.6808510638298, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790162, Length=229, Percent_Identity=25.3275109170306, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786703, Length=197, Percent_Identity=32.48730964467, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788225, Length=219, Percent_Identity=25.5707762557078, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=233, Percent_Identity=24.8927038626609, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693107, Length=228, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786398, Length=225, Percent_Identity=26.6666666666667, Blast_Score=61, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28574744, Length=213, Percent_Identity=25.8215962441315, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: =3.6.3.33 [H]

Molecular weight: Translated: 27325; Mature: 27194

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLMLLEDVAESHRLGPLSGEIRAGEILHLVGPNGAGKSTLLARMAGLTTGPGRVYFAG
CCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEH
SVLESWSATKLALHRAYLAQQQNPPFAMPVWHFLSLHQPDKTRTDLLTKMAESLGLADKL
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
ERPANQLSGGEWQRVRLAAVVLQIDPKSNPHGQLLLLDEPMNSLDVAQQNALDRILSHLC
HHHHHHCCCCCHHHEEEEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QQGIAVVMSSHDLNHTLHHAHRAWLLKQGKLLAGGPREDVLTPPNLALAYGLNFRRLDIE
HHHHEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCEEEEEEC
GHRMLISTS
CCEEEEECC
>Mature Secondary Structure 
TQLMLLEDVAESHRLGPLSGEIRAGEILHLVGPNGAGKSTLLARMAGLTTGPGRVYFAG
CHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEH
SVLESWSATKLALHRAYLAQQQNPPFAMPVWHFLSLHQPDKTRTDLLTKMAESLGLADKL
HHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
ERPANQLSGGEWQRVRLAAVVLQIDPKSNPHGQLLLLDEPMNSLDVAQQNALDRILSHLC
HHHHHHCCCCCHHHEEEEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QQGIAVVMSSHDLNHTLHHAHRAWLLKQGKLLAGGPREDVLTPPNLALAYGLNFRRLDIE
HHHHEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCEEEEEEC
GHRMLISTS
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA